| Budget Amount *help |
¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
PCR-based genetic testing has found its applications in many fields such as detection of infectious diseases, food poisoning, etc. Especially, on-site rapid DNA detection is required. In this study, the centrifugation assisted thermal convection PCR was proposed. The PCR solution could be driven by thermal convection and continuously exchang high/low temperatures in a ring structured micro-channel without the use of typical syringe pump. After optimization of other conditions (chip coating, bubble prevention, temparature, flow rate, etc.), the on-chip DNA amplification was successfully achieved from 120 pg of initial DNA within only 15 min. From this result, the methods and conditions for the centrifugation assisted thermal convection PCR could be established. I hope this result will be step toward the practical use of the easy and rapid PCR device for all users.
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