| Project/Area Number |
25630380
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kobe University |
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Principal Investigator |
TATSUO Maruyama 神戸大学, 工学(系)研究科(研究院), 准教授 (30346811)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 自己組織化 / ナノファイバー / 細胞内送達 / ガン細胞 / 選択的殺傷 / ペプチド / 超分子 / ペプチド脂質 |
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| Outline of Final Research Achievements |
We report cancer cell death initiated by the intracellular molecular self-assembly of a peptide lipid, which served as a gelator precursor. The gelator precursor was designed to form nanofibres via molecular self-assembly, after cleavage by a cancer-related enzyme (matrix metalloproteinase-7, MMP-7), leading to hydrogelation. The precursor exhibited remarkable cytotoxicity to 5 different cancer cell lines, while it exhibited low cytotoxicity to normal cells that secreted small amounts of MMP-7. Cancer cells secrete excessive amounts of MMP-7, which converted the precursor into a supramolecular gelator prior to its uptake by the cells. Once inside the cells, the supramolecular gelator formed a gel via molecular self-assembly, exerting stress upon the cancer cells. The present study thus describes a new drug where molecular self-assembly acts as the mechanism of cytotoxicity.
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