| Project/Area Number |
25640017
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Neurophysiology / General neuroscience
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
YOUICHI Iwai 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (40332332)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | グリア光遺伝学 / Gタンパク質共役型受容体（GPCR） / アストロサイト / グリア－ニューロン相互作用 / グリア由来可塑性因子 / 運動学習 / G蛋白質共役型受容体 / グリア-ニューロン相互作用 |
|---|
| Outline of Final Research Achievements |
In order to directly examine astrocyte-dependent signaling and motor learning, we have generated transgenic mice in which OptoXR, an optically activatable Gq-coupled receptor (Airan et al. 2009), is selectively expressed in astrocytes. We successfully confirmed that Ca2+ elevations were triggered in OptoXR-expressing astrocytes by brief illumination of blue light in anesthetized conditions. We found that astrocytic Ca2+ propagation is induced depending on the stimulus condition, as naive astrocytes also exhibited Ca2+ surges with a delay after the activation of the neighboring OptoXR-expressing astrocytes. We further observed that the surrounding neurons could be activated following the illumination. We are currently exploring these astrocyte-dependent signaling mechanisms, and conducting behavioral experiments in which motor cortex of the transgenic mice are illuminated during motor-learning.
|
