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Contribution of brain-specific miRs in the neuronal and glial function and possible transmission of miRs between cells.

Research Project

Project/Area Number 25640019
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurophysiology / General neuroscience
Research InstitutionNational Center of Neurology and Psychiatry

Principal Investigator

NUMAKAWA Tadahiro  独立行政法人国立精神・神経医療研究センター, 神経研究所 疾病研究第三部, 客員研究員 (40425690)

Co-Investigator(Renkei-kenkyūsha) HASHIDO Kazuo  独立行政法人国立精神・神経医療研究センター, 神経研究所 ラジオアイソトープ管理室, 室長 (70270650)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsmicroRNA / 神経伝達 / 神経栄養因子 / 脳神経疾患 / 神経科学 / マイクロRNA / エクソソーム
Outline of Final Research Achievements

It has been suggested that changed blood level of miRs is beneficial biomarker of diseases including brain dysfunction. In this study, we examined function of brain-specific miRs in the neuronal and glial system, and a possibility of miR transmission among cells. We found that upregulation of several brain-specific miRs in exosome released from cultured cortical neurons after application of BDNF, which is essential growth factor for neural function in the central nervous system neurons. On the other hand, bFGF, which is involved in glial survival and differentiation, increased the levels of miR-134. Furthermore, we found that the bFGF/miR-134 system is important for maturation of astroglia, including upregulation of glutamate transporter.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (37 results)

All 2015 2014 2013 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Open Access: 2 results) Presentation (25 results) (of which Invited: 3 results)

  • [Journal Article] Basic fibroblast growth factor induces miR-134 upregulation in astrocyte for cell maturation2015

    • Author(s)
      Numakawa T, Nakajima S, Yamamoto N, Ooshima Y, Odaka H, Hashido K, Adachi N, Kunugi H.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 456 Issue: 1 Pages: 465

    • DOI

      10.1016/j.bbrc.2014.11.108

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New insight in expression, transport, and secretion of brain-derived neurotrophic factor: Implications in brain-related diseases.2014

    • Author(s)
      Adachi N, Numakawa T, Richards M, Nakajima S, Kunugi H.
    • Journal Title

      World J Biol Chem.

      Volume: 5 Issue: 4 Pages: 409-428

    • DOI

      10.4331/wjbc.v5.i4.409

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible protective action of neurotrophic factors and natural compounds against common neurodegenerative diseases.2014

    • Author(s)
      Numakawa T.
    • Journal Title

      Neural Regen Res.

      Volume: 9 Issue: 16 Pages: 1506-1508

    • DOI

      10.4103/1673-5374.139474

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role of brain-derived neurotrophic factor (BDNF) in comorbid depression: Possible linkage with steroid hormones, cytokines, and nutrition2014

    • Author(s)
      Numakawa T, Richards M, Nakajima S, Adachi N, Furuta M, Odaka H, Kunugi H.
    • Journal Title

      Front Psychiatry.

      Volume: 5 Issue: SEP Pages: 136-136

    • DOI

      10.3389/fpsyt.2014.00136

    • URL

      https://pure.teikyo.jp/en/publications/9dee5d2c-de80-4bc9-97df-26d512f4a1e9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cabergoline, dopamine D2 receptor agonist, prevents neuronal cell death under oxidative stress via reducing excitotoxicity.2014

    • Author(s)
      Odaka H, Numakawa T, Adachi N, Ooshima Y, Nakajima S, Katanuma Y, Inoue T, Kunugi H.
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 6 Pages: e99271-e99271

    • DOI

      10.1371/journal.pone.0099271

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phencyclidine rapidly decreases neuronal mRNA of brain-derived neurotrophic factor2014

    • Author(s)
      Katanuma Y, Numakawa T, Adachi N, Yamamoto N, Ooshima Y, Haruki O, Takafumi I, Kunugi H.
    • Journal Title

      Synapse.

      Volume: 68 Issue: 6 Pages: 257

    • DOI

      10.1002/syn.21735

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Impairments in brain-derived neurotrophic factor-induced glutamate release in cultured cortical neurons derived from rats with intrauterine growth retardation: Possible involvement of suppression of TrkB/phospholipase C-γ activation2014

    • Author(s)
      Numakawa T, Matsumoto T, Ooshima Y, Chiba S, Furuta M, Izumi A, Ninomiya-Baba M, Odaka H, Hashido K, Adachi N, Kunugi H.
    • Journal Title

      Neurochem Res.

      Volume: 39 Issue: 4 Pages: 785

    • DOI

      10.1007/s11064-014-1270-x

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phosphatase-mediated intracellular signaling contributes to neuroprotection by flavonoids of iris tenuifolia2014

    • Author(s)
      Jalsrai A, Numakawa T, Ooshima Y, Adachi N, Kunugi H.
    • Journal Title

      Am J Chin Med.

      Volume: 42 Issue: 1 Pages: 119

    • DOI

      10.1142/s0192415x14500086

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Teneurin-4 promotes cellular protrusion formation and neurite outgrowth through focal adhesion kinase signaling2014

    • Author(s)
      Suzuki N, Numakawa T, Chou J, de Vega S, Mizuniwa C, Sekimoto K, Adachi N, Kunugi H, Arikawa-Hirasawa E, Yamada Y, Akazawa C.
    • Journal Title

      FASEB J.

      Volume: 28 Issue: 3 Pages: 1386

    • DOI

      10.1096/fj.13-241034

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Estrogen, predominantly via estrogen receptor α, attenuates postpartum-induced anxiety- and depression-like behaviors in female rats2013

    • Author(s)
      Furuta M, Numakawa T, Chiba S, Ninomiya M, Kajiyama Y, Adachi N, Akema T, Kunugi H.
    • Journal Title

      Endocrinology.

      Volume: 154 Issue: 10 Pages: 3807

    • DOI

      10.1210/en.2012-2136

    • URL

      https://pure.teikyo.jp/en/publications/5dff371f-8b60-46b4-973b-01a9754df6c9

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Neurotrophin BDNF and novel molecular targets in depression pathogenesis.2013

    • Author(s)
      Numakawa T, Nakajima S, Adachi N, Richards M, Kunugi H.
    • Journal Title

      J. Neurol. Transl. Neurosci.

      Volume: 1(3) Pages: 1021-1021

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Response of Glucocorticoids to Stress and Functional Alteration of Brain-derived Neurotrophic Factor.2013

    • Author(s)
      Numakawa T.
    • Journal Title

      J. Neurol. Transl. Neurosci.

      Volume: 1 Pages: 1004-1004

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] アストログリア細胞においてbFGF投与で増加したマイクロRNAの機能的役割2014

    • Author(s)
      沼川 忠広、中島 進吾、山本 宣子、大島 淑子、小高 陽樹、橋戸 和夫、安達 直樹、功刀 浩
    • Organizer
      第36回日本生物学的精神医学会 第57回日本神経化学会大会 合同年会
    • Place of Presentation
      奈良県文化会館/奈良県新公会堂(奈良県)
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] 経口グルコース負荷後のERKの変化2014

    • Author(s)
      中島 進吾、沼川 忠広、安達 直樹、功刀浩
    • Organizer
      第36回日本生物学的精神医学会 第57回日本神経化学会大会 合同年会
    • Place of Presentation
      奈良県文化会館/奈良県新公会堂(奈良県)
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] Possible of CD63 in synaptic function of rat cortical neurons.2014

    • Author(s)
      吉村 文, 沼川忠広, 中島進吾, 安達直樹, 川又理樹, 落谷孝広, 功刀 浩, 玉井淑貴
    • Organizer
      第36回日本生物学的精神医学会 第57回日本神経化学会大会 合同年会
    • Place of Presentation
      奈良県文化会館/奈良県新公会堂(奈良県)
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] グルココルチコイドによる脳由来神経栄養因子(BDNF)の細胞内輸送制御2014

    • Author(s)
      安達 直樹、沼川 忠広、中島 進吾、大島 淑子、小高 陽樹、片沼 祐介、功刀 浩
    • Organizer
      第36回日本生物学的精神医学会 第57回日本神経化学会大会 合同年会
    • Place of Presentation
      奈良県文化会館/奈良県新公会堂(奈良県)
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] 培養大脳皮質ニューロンに対するカベルゴリン(ドーパミンD2受容体アゴニスト)の神経保護効果の解析2014

    • Author(s)
      小髙 陽樹、沼川 忠広、安達 直樹、大島 淑子、中島 進吾、片沼 佑介、井上 貴文、 功刀浩
    • Organizer
      第36回日本生物学的精神医学会 第57回日本神経化学会大会 合同年会
    • Place of Presentation
      奈良県文化会館/奈良県新公会堂(奈良県)
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] フェンサイクリジン短期曝露は大脳皮質ニューロンのBDNF mRNA発現を急速に低下させる2014

    • Author(s)
      片沼佑介、沼川忠広、安達直樹、山本宣子、大島淑子、小高陽樹、井上貴文、功刀浩
    • Organizer
      第37回日本神経科学大会 Neuroscience 2014.
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Glial miR expression following basic fibroblast growth factor application in astrocytes2014

    • Author(s)
      Yamamoto N, Numakawa T, Nakajima S, Ooshima Y, Odaka H, Hashido K, Adachi N, Kunugi H.
    • Organizer
      第37回日本神経科学大会 Neuroscience 2014.
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] High fat diet-induced deficiency of prepulse inhibition in mice2014

    • Author(s)
      Wakabayashi C, Numakawa T, Ooshima Y, Hattori K, Kunugi H.
    • Organizer
      第37回日本神経科学大会 Neuroscience 2014.
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Decreased release of neurotransmitter induced by BDNF in cultured cortical neurons from IUGR(intrauterine growth retardation) rats2014

    • Author(s)
      Ooshima Y, Numakawa T, Matsumoto T, Chiba S, Furuta M,Izumi A, Ninomiya-Baba M, Odaka H, Hashido K, Adachi N, Kunugi H.
    • Organizer
      第37回日本神経科学大会 Neuroscience 2014.
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Influence of chronic glucocorticoid exposure on proliferation and differentiation of rat neural stem cells in vitro2014

    • Author(s)
      Odaka H, Numakawa T, Yoshimura A, Adachi N, Nakajima S, Era T, Inoue T, Kunugi H.
    • Organizer
      熊本医学・生物科学国際シンポジウム
    • Place of Presentation
      熊本市医師会館(熊本県)
    • Year and Date
      2014-09-04 – 2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] The effect of IL-6 on expression of microRNAs in rat embryonic neural stem cells during their differentiation.2014

    • Author(s)
      Yoshimura A, Numakawa T, Odaka H, Tamai Y, Era T,
    • Organizer
      熊本医学・生物科学国際シンポジウム
    • Place of Presentation
      熊本市医師会館(熊本県)
    • Year and Date
      2014-09-04 – 2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] The mechanisms behind deficits in BDNF function after phencyclidine exposure2014

    • Author(s)
      Adachi N, Numakawa T, Kumamaru E, Itami C, Chiba S, Iijima Y, Richards M, Katoh-Semba R, Kunugi H.
    • Organizer
      CSHA/NGF2014 Joint Conference on Nerve Growth Factor and Related Neurotrophic Factors: Emerging Concepts, New Mechanism, Novel Technologies
    • Place of Presentation
      Suzhou, PR China
    • Year and Date
      2014-06-23 – 2014-06-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] MicroRNA induction by basic fibroblast growth factor in pure astroglial cultures.2014

    • Author(s)
      Nakajima S, Numakawa T, Yamamoto N, Yoshiko O, Odaka H, Hashido K, Adachi N, Kunugi H.
    • Organizer
      CSHA/NGF2014 Joint Conference on Nerve Growth Factor and Related Neurotrophic Factors: Emerging Concepts, New Mechanism, Novel Technologies
    • Place of Presentation
      Suzhou, PR China
    • Year and Date
      2014-06-23 – 2014-06-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Stress regulation of glucocorticoids and role of neurotrophic factor in neurons: Implications for depression2013

    • Author(s)
      Tadahiro Numakawa
    • Organizer
      11th World Congress of Biological Psychiatry
    • Place of Presentation
      Kyoto, Japan, Kyoto International Conference Center
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Glucocorticoids affect the intracellular transport of BDNF in cortical neurons2013

    • Author(s)
      Naoki Adachi, Tadahiro Numakawa, Yoshiko Ooshima, Hiroshi Kunugi.
    • Organizer
      Neuro2013
    • Place of Presentation
      京都府 京都市 国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Aldosterone enhanced release of neurotransmitter in cultured neurons2013

    • Author(s)
      Akari Okamoto, Tadahiro Numakawa., Naoki Adachi, Yoshinori Masuo, Hiroshi Kunugi.
    • Organizer
      Neuro2013
    • Place of Presentation
      京都府 京都市 国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Protective effect of cabergoline, a dopamine D2 receptor agonist, on cultured neurons.2013

    • Author(s)
      Haruki Odaka, Tadahiro Numakawa, Naoki Adachi, Yoshiko Ooshima, Takafumi Inoue, and Horoshi Kunugi.
    • Organizer
      Neuro2013
    • Place of Presentation
      京都府 京都市 国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Possible involvement of shp2 in an influence of glucocorticoid on neurotrophin-dependent synapse regulation2013

    • Author(s)
      Tadahiro Numakawa, Naoki Adachi, Emi Kumamaru, Yoshiko Ooshima, and Hiroshi Kunugi.
    • Organizer
      Neuro2013
    • Place of Presentation
      京都府 京都市 国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] The Effect of Phencyclidine on BDNF mRNA Expression in Rat Cultured Cortical Neurons.2013

    • Author(s)
      Yusuke Katanuma, Tadahiro Numakawa, Naoki Adachi, Takafumi Inoue, Hiroshi Kunugi.
    • Organizer
      Neuro2013
    • Place of Presentation
      京都府 京都市 国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Fibroblast growth factor-dependent upregulation of microRNAs in cultured neuronal and glial cells.2013

    • Author(s)
      Noriko Yamamoto, Tadahiro Numakawa, Yoshiko Ooshima, Soichiro Kishi, Kazuo Hashido, Naoki Adachi, and Hiroshi Kunugi.
    • Organizer
      Neuro2013
    • Place of Presentation
      京都府 京都市 国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Possible change in neurotrophin function in decreased weight rats model.2013

    • Author(s)
      Yoshiko Ooshima, Tadahiro Numakawa, Naoki Adachi, and Hiroshi Kunugi.
    • Organizer
      Neuro2013
    • Place of Presentation
      京都府 京都市 国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Teneurin-4 Positively Regulates Neural Cell Process Formation and Synaptogenesis2013

    • Author(s)
      Nobuharu Suzuki, Tadahiro Numakawa, Joshua Chou, Susana de Vega, Chihiro Mizuniwa, Kaori Sekimoto, Naoki Adachi, Hiroshi Kunugi, Eri Arikawa-Hirasawa, Yoshihiko Yamada, and Chihiro Akazawa.
    • Organizer
      Neuro2013
    • Place of Presentation
      京都府 京都市 国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] 大脳皮質ニューロンにおけるGLP-1とBDNFとの相互作用2013

    • Author(s)
      中島進吾、沼川忠広、安達直樹、功刀浩
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      神奈川県 横浜市 パシフィコ横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] 神経障害におけるストレスおよび栄養因子BDNFの役割について2013

    • Author(s)
      沼川忠広
    • Organizer
      大阪大学 蛋白質研究所 蛋白研リトリート講演
    • Place of Presentation
      兵庫県淡路島 夢舞台
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Glucocorticoid stress, neurotrophin, and pathogenesis of psychiatric disorders

    • Author(s)
      Tadahiro Numakawa
    • Organizer
      熊本大学リエゾンラボ研究会/リーディングプログラム:HIGO最先端研究セミナー
    • Place of Presentation
      熊本大学
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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