Contribution of brain-specific miRs in the neuronal and glial function and possible transmission of miRs between cells.
Project/Area Number |
25640019
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | National Center of Neurology and Psychiatry |
Principal Investigator |
NUMAKAWA Tadahiro 独立行政法人国立精神・神経医療研究センター, 神経研究所 疾病研究第三部, 客員研究員 (40425690)
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Co-Investigator(Renkei-kenkyūsha) |
HASHIDO Kazuo 独立行政法人国立精神・神経医療研究センター, 神経研究所 ラジオアイソトープ管理室, 室長 (70270650)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | microRNA / 神経伝達 / 神経栄養因子 / 脳神経疾患 / 神経科学 / マイクロRNA / エクソソーム |
Outline of Final Research Achievements |
It has been suggested that changed blood level of miRs is beneficial biomarker of diseases including brain dysfunction. In this study, we examined function of brain-specific miRs in the neuronal and glial system, and a possibility of miR transmission among cells. We found that upregulation of several brain-specific miRs in exosome released from cultured cortical neurons after application of BDNF, which is essential growth factor for neural function in the central nervous system neurons. On the other hand, bFGF, which is involved in glial survival and differentiation, increased the levels of miR-134. Furthermore, we found that the bFGF/miR-134 system is important for maturation of astroglia, including upregulation of glutamate transporter.
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] Impairments in brain-derived neurotrophic factor-induced glutamate release in cultured cortical neurons derived from rats with intrauterine growth retardation: possible involvement of suppression of TrkB/phospholipase C-γ activation.2014
Author(s)
Numakawa T, Matsumoto T, Ooshima Y, Chiba S, Furuta M, Izumi A, Ninomiya-Baba M, Odaka H, Hashido K, Adachi N, Kunugi H.
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Journal Title
Neurochem Res.
Volume: 39(4)
Issue: 4
Pages: 785-92
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Teneurin-4 promotes cellular protrusion formation and neurite outgrowth through focal adhesion kinase signaling.2014
Author(s)
Suzuki N, Numakawa T, Chou J, de Vega S, Mizuniwa C, Sekimoto K, Adachi N, Kunugi H, Arikawa-Hirasawa E, Yamada Y, Akazawa C.
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Journal Title
FASEB J.
Volume: 28(3)
Issue: 3
Pages: 1386-97
DOI
Related Report
Peer Reviewed
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[Presentation] The mechanisms behind deficits in BDNF function after phencyclidine exposure2014
Author(s)
Adachi N, Numakawa T, Kumamaru E, Itami C, Chiba S, Iijima Y, Richards M, Katoh-Semba R, Kunugi H.
Organizer
CSHA/NGF2014 Joint Conference on Nerve Growth Factor and Related Neurotrophic Factors: Emerging Concepts, New Mechanism, Novel Technologies
Place of Presentation
Suzhou, PR China
Year and Date
2014-06-23 – 2014-06-27
Related Report
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[Presentation] MicroRNA induction by basic fibroblast growth factor in pure astroglial cultures.2014
Author(s)
Nakajima S, Numakawa T, Yamamoto N, Yoshiko O, Odaka H, Hashido K, Adachi N, Kunugi H.
Organizer
CSHA/NGF2014 Joint Conference on Nerve Growth Factor and Related Neurotrophic Factors: Emerging Concepts, New Mechanism, Novel Technologies
Place of Presentation
Suzhou, PR China
Year and Date
2014-06-23 – 2014-06-27
Related Report
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[Presentation] Teneurin-4 Positively Regulates Neural Cell Process Formation and Synaptogenesis2013
Author(s)
Nobuharu Suzuki, Tadahiro Numakawa, Joshua Chou, Susana de Vega, Chihiro Mizuniwa, Kaori Sekimoto, Naoki Adachi, Hiroshi Kunugi, Eri Arikawa-Hirasawa, Yoshihiko Yamada, and Chihiro Akazawa.
Organizer
Neuro2013
Place of Presentation
京都府 京都市 国立京都国際会館
Related Report
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