Molecular mechanism of miRNA-mediated regulation of emotional behaviors
Project/Area Number |
25640033
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurochemistry/Neuropharmacology
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Research Institution | Osaka University |
Principal Investigator |
NAKAZAWA Takanobu 大阪大学, 薬学研究科(研究院), 特任准教授 (00447335)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | マイクロRNA / 報酬系 / 情動 / 薬物依存 / モデルマウス / シグナル伝達 / 不妊 / 遺伝子欠損マウス |
Outline of Final Research Achievements |
miRNAs are highly expressed in the central nervous system, however, their roles in higher brain functions remain unclear. In this study, using the cocaine-induced microRNA knockout mice, I examined the function of the miRNA in emotional behaviors and its associated molecular mechanisms. In addition to reward system, I found that the knockout mice exhibited abnormal emotional behaviors in the elevated plus maze test and forced swim test. Using microarray methods, I also found that the miRNA-mediated regulation of Rho-kinase was involved in these behavioral abnormalities. Interestingly, Src kinase and integrin-mediated pathway regulated the expression of the miRNA. Taken together, Rho-kinase and Src-kinase/integrin-mediated signaling pathways are important for the cocaine-induced miRNA-mediated regulation of emotional behaviors. In addition, female infertility was found in the miRNA knockout mice.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] Identification of the role of BMP and TGF-β signaling in the trajectory of serotonergic differentiation in a rapid assay in mouse embryonic stem cells in vitro.2015
Author(s)
Yamasaki A, Kasai A, Toi A, Kurita M, Kimoto S, Hayata-Takano A, Nakazawa T, Nagayasu K, Shintani N, Hashimoto R, Ito A, Meltzer HY, Ago Y, Waschek JA, Onaka Y, matsuda T, Baba A, Hashimoto H.
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Journal Title
Journal of Neurochemistry,
Volume: 132(4)
Issue: 4
Pages: 418-428
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] PACAP Enhances Axon Outgrowth in Cultured Hippocampal Neurons to a Comparable Extent as BDNF.2015
Author(s)
Ogata K, Shintani N, Hayata-takano A, Kamo T, Higashi S, Seiriki K, Momosaki H, Vaudy D, Vaudy H, Galas L, Kasai A, Nagayasu K, Nakazawa T, Hashimoto R, Ago Y, Matsuda T, Baba A, Hashimoto H.
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Journal Title
PLoS One
Volume: 10
Issue: 3
Pages: e0120526-e0120526
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] CRTH2, a prostaglandin D2 receptor, mediates depression-related behavior in mice2015
Author(s)
Yusuke Onaka, Norihito Shintani, Takanobu Nakazawa, Ryota Haba, Yukio Ago, Hyper Wang, Takuya Kanoh, Atsuko Hayata-Takano, Hiroyuki Hirai, Kin-ya Nagata, Masataka Nakamura, Ryota Hashimoto, Toshio Matsuda, James A. Waschek, Atsushi Kasai, Kazuki Nagayasu, Akemichi Baba, Hitoshi Hashimoto
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Journal Title
Behavioural Brain Research
Volume: 284
Pages: 131-137
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Simultaneous neuron- and astrocyte-specific fluorescent marking.2015
Author(s)
Schulze W, Hayata-Takano A, Kamo T, Nakazawa T, Nagayasu K, Kasai A, Seiriki K, Shintani N, Ago Y, Farfan C, Hashimoto R, Baba A, Hashimoto H.
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 459
Issue: 1
Pages: 81-86
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Dopamine-induced tyrosine phosphorylation of NR2B (Tyr1472) is essential for ERK1/2 activation and processing of novel taste information.2014
Author(s)
David, O., Barrera, I., Chinnakkaruppan, A., Kaphzan, H., Nakazawa, T., Yamamoto, T. & Rosenblum, K.
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Journal Title
Frontiers in molecular neuroscience
Volume: 7
Pages: 66-66
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] NR2B Phosphorylation at Tyrosine 1472 Contributes to Brain Injury in a Rodent Model of Neonatal Hypoxia-Ischemia.2014
Author(s)
Knox, R., Brennan-Minnella, A. M., Lu, F. X., Yang, D. N., Nakazawa, T., Yamamoto, T., Swanson, R. A., Ferriero, D. M. & Jiang, X. N.
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Journal Title
Stroke
Volume: 45
Issue: 10
Pages: 3040-3047
DOI
Related Report
Peer Reviewed
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[Journal Article] Behavioral characterization of mice overexpressing human dysbindin-12014
Author(s)
Shintani N, Onaka Y, Hashimoto R, Takamura H, Nagata T, Umeda-Yano S, Mouri A, Mamiya T, Haba R, Matsuzaki S, Katayama T, Yamamori H, Nakazawa T, Nagayasu K, Ago Y, Yagasaki Y, Nabeshima T, Takeda M, Hashimoto H
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Journal Title
Molecular Brain
Volume: 7(1)
Issue: 1
Pages: 74-74
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Central CRTH2, a second prostaglandin D2 receptor, mediates emotional impairment in the lipopolysaccharide and tumor-induced sickness behavior model.2014
Author(s)
Haba R, Shintani N, Onaka Y, Kanoh T, Wang H, Takenaga R, Hayata A, Hirai H, Nagata KY, Nakamura M, Kasai A, Haghi moto R, Nagayasu K, Nakazawa T, Hashimoto H, Baba A.
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Journal Title
J Neurosci.
Volume: 34
Issue: 7
Pages: 2514-2523
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Protocadherin 17 regulates presynaptic assembly in topographic corticobasal Ganglia circuits2013
Author(s)
Hoshina N, Tanimura A, Yamasaki M, Inoue T, Fukabori R, Kuroda T, Yokoyama K, Tezuka T, Sagara H, Hirano S, Kiyonari H, Takada M, Kobayashi K, Watanabe M, Kano M, Nakazawa T, Yamamoto T
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Journal Title
Neuron
Volume: 78(5)
Issue: 5
Pages: 839-854
DOI
Related Report
Peer Reviewed
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