| Project/Area Number |
25640033
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Osaka University |
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Principal Investigator |
NAKAZAWA Takanobu 大阪大学, 薬学研究科(研究院), 特任准教授 (00447335)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | マイクロRNA / 報酬系 / 情動 / 薬物依存 / モデルマウス / シグナル伝達 / 不妊 / 遺伝子欠損マウス |
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| Outline of Final Research Achievements |
miRNAs are highly expressed in the central nervous system, however, their roles in higher brain functions remain unclear. In this study, using the cocaine-induced microRNA knockout mice, I examined the function of the miRNA in emotional behaviors and its associated molecular mechanisms. In addition to reward system, I found that the knockout mice exhibited abnormal emotional behaviors in the elevated plus maze test and forced swim test. Using microarray methods, I also found that the miRNA-mediated regulation of Rho-kinase was involved in these behavioral abnormalities. Interestingly, Src kinase and integrin-mediated pathway regulated the expression of the miRNA. Taken together, Rho-kinase and Src-kinase/integrin-mediated signaling pathways are important for the cocaine-induced miRNA-mediated regulation of emotional behaviors. In addition, female infertility was found in the miRNA knockout mice.
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