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Development of an in situ visible cell-free assay system for neurotransmitter release

Research Project

Project/Area Number 25640036
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurochemistry/Neuropharmacology
Research InstitutionKobe University

Principal Investigator

SAKISAKA TOSHIAKI  神戸大学, 医学(系)研究科(研究院), 教授 (80359843)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO YASUNORI  神戸大学, 大学院医学研究科, 准教授 (30467659)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords神経伝達物質 / シナプス小胞 / 膜融合
Outline of Final Research Achievements

The main process of synaptic transmission form one neuron to another neuron is mediated by neurotransmitter release. The entry of Ca2+ into nerve terminals initiates the series of molecular events that culminates with fusion of the synaptic vesicle and release of neurotransmitter into the synaptic cleft. The presynaptic membrane curvature change has been shown to be involved in the synaptic vesicle fusion. Here we identify membrane-shaping proteins, Arl6IP1 and TMCC3. We also identify an Arl6IP1-interacting protein TMEM33. TMEM33 suppresses the membrane-shaping activity of Arl6IP1. On the other hand, TMCC3 has membrane-laminating activity through interacting with membrane-shaping proteins, reticulon family proteins.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (14 results)

All 2016 2015 2014 2013 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 4 results,  Open Access: 3 results) Presentation (3 results) Book (2 results) Remarks (3 results)

  • [Journal Article] The Emerging Role of Calcium-modulating Cyclophilin Ligand (CAML) in Posttranslational Insertion of Tail-anchored Proteins into the Endoplasmic Reticulum Membrane.2015

    • Author(s)
      Yamamoto, Y., and Sakisaka, T.
    • Journal Title

      The Journal of Biochemistry

      Volume: 印刷中 Issue: 6 Pages: 419-429

    • DOI

      10.1093/jb/mvv035

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Crystal structure of afadin PDZ domain-nectin-3 complex shows the structural plasticity of the ligand-binding site.2015

    • Author(s)
      Fujiwara, Y., Goda, N., Tamashiro, T., Narita, H., Satomura, K., Tenno, T., Nakagawa, A., Oda, M., Suzuki, M., Sakisaka, T., Takai, Y., and Hiroaki, H.
    • Journal Title

      Protein Science

      Volume: 24 Pages: 376-385

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cytoarchitecture of the olfactory bulb in the laggard mutant mouse.2014

    • Author(s)
      Yunus J, Setsu T, Kikkawa S, Sakisaka T, Terashima T.
    • Journal Title

      Neuroscience

      Volume: 275 Pages: 259-271

    • DOI

      10.1016/j.neuroscience.2014.06.011

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification and Characterization of TMEM33 as a Reticulon-binding Protein.2014

    • Author(s)
      Urade, T., Yamamoto, Y., Zhang, X., Ku, Y., and Sakisaka, T.
    • Journal Title

      Kobe Journal of Medical Sciences

      Volume: 60

    • NAID

      110009842833

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] VAP-B Binds to Rab3GAP1 at the ER: Its Implication in Nuclear Envelope Formation through the ER-Golgi Intermediate Compartment.2014

    • Author(s)
      Hantan, D., Yamamoto, Y., and Sakisaka, T.
    • Journal Title

      Kobe Journal of Medical Sciences

      Volume: 60

    • NAID

      110009831834

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Arl6IP1 has the ability to shape the mammalian ER membrane in a reticulon-like fashion.2014

    • Author(s)
      Yamamoto, Y., Yoshida, A., Miyazaki, N., Iwasaki, K., and Sakisaka, T.
    • Journal Title

      Biochemical Journal

      Volume: 458 Issue: 1 Pages: 69-79

    • DOI

      10.1042/bj20131186

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 髄鞘低形成と皮質形成異常を示す新規突然変異マウスlaggard2016

    • Author(s)
      匂坂 敏朗、寺島 俊雄
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレット福島
    • Year and Date
      2016-03-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 小胞体の膜形態制御と翻訳後膜挿入の分子機構2014

    • Author(s)
      山本 泰憲、匂坂 敏朗
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2014-10-15
    • Related Report
      2014 Research-status Report
  • [Presentation] Reticulon結合タンパク質TMEM33の同定と性状解析2013

    • Author(s)
      張 霞, 山本 泰憲, 浦出 剛史, 匂坂 敏朗
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report
  • [Book] DOJIN BIOSCIENCEシリーズ『メンブレントラフィック』 PartIII:高次生体機能を支えるメンブレントラフィック 10章 神経伝達物質放出を支えるメンブレントラフィック2016

    • Author(s)
      山本 泰憲、匂坂 敏朗
    • Publisher
      化学同人
    • Related Report
      2015 Annual Research Report
  • [Book] Presynaptic Terminals2015

    • Author(s)
      Yamamoto, Y., and Sakisaka, T.
    • Total Pages
      365
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report
  • [Remarks]

    • URL

      http://www.med.kobe-u.ac.jp/membrd/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 神戸大学大学院医学研究科 生理学・細胞生物学講座 膜動態学分野

    • URL

      http://www.med.kobe-u.ac.jp/membrd/

    • Related Report
      2014 Research-status Report
  • [Remarks] 神戸大学大学院医学研究科 生理学・細胞生物学講座 膜動態学分野

    • URL

      http://www.med.kobe-u.ac.jp/membrd/

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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