| Project/Area Number |
25640046
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKASHI Kuramoto 京都大学, 医学(系)研究科(研究院), 准教授 (20311409)
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| Co-Investigator(Renkei-kenkyūsha) |
三浦 岳 九州大学, 医学研究科, 教授 (10324617)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ラット / 変異体 / 毛色 / Kit / Zeb2 / メラノサイト / ノックアウトラット / パターン形成 / ドパクロームトートメラーゼ |
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| Outline of Final Research Achievements |
Rreaction-diffusion (RD) model is one of the best-known theoretical models used to explain self-regulated pattern formation. In this study, we tried to establish two rat coat pattern mutations (hooded and Downunder) as an in vivo model to evaluate the RD model in mammals. The hooded rats have a pattern in which the entire ventral surface is white. Dorsally pigmentation is limited to the head and shoulders and a mid-dorsal stripe. The downunder pattern manifests when rats are homozygous for the hooded mutation. The downunder rats have pigmentation in ventral part of the trunk in addition to the hooded pattern. We created a F344-Tg(Dct-LacZ) transgenic rat line to monitor the development of the melanocytes. We mapped the Downunder locus finely to about 1 Mb genomic region on rat chromosome 3 and found the Zeb2 gene as a good candidate for the Downunder. Using the transgenic rats, we could monitor the development of the melanocytes in the hooded and Dwonunder embryos in the further study.
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