| Project/Area Number |
25640047
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka University |
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Principal Investigator |
YAGI Takeshi 大阪大学, 生命機能研究科, 教授 (10241241)
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| Co-Investigator(Renkei-kenkyūsha) |
UCHIMURA Arikuni 大阪大学, 生命機能研究科, 助教 (20513063)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 突然変異 / マウス / 疾患モデル / 進化 / 生殖系列 / DNA複製 / 遺伝子 / 量的形質 / 哺乳類 / DNAポリメラーゼ |
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| Outline of Final Research Achievements |
The germline mutation rate is an important parameter that affects genetic variation and the rate of evolution. In this project, we studied genome-wide mutation rates and their long-term effects on phenotype in more than 20 generations of wild-type C57BL/6 mice and mutator mice, which have high DNA replication error rates. The mutation rate in mutator mice was 17 times that in wild-type mice. Abnormal phenotypes were 4-fold more frequent in the mutator lines than in the wild-type lines. After several generations, the mutator mice reproduced at substantially lower rates than the controls, exhibiting low pregnancy rates, lower survival rates, and smaller litter sizes, and many of the breeding lines died out.
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