Functional analysis of chromosome specific clustered trap region (CSCT).

• Project/Area Number: 25640052
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Kumamoto University
• Principal Investigator: ARAKI MASATAKE 熊本大学, 生命資源研究・支援センター, 准教授 (80271609)
• Co-Investigator(Kenkyū-buntansha): ARAKI Kimi 熊本大学, 生命資源研究・支援センター, 教授 (90211705)
• Research Collaborator: YOSHINOBU Kumiko 熊本大学, 生命資源研究・支援センター, 助教 (20274730)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 遺伝子トラップ / CSCT / EGTC / ゲノム編集 / 染色体相同組換え / 減数分裂 / クラスター遺伝子 / 染色体特異的 / マウス

Outline of Final Research Achievements

We found new genome element, CSCT (Chromosome Specific Clustered Trap region).
Using CRISPR/Cas9 system, CSCT13 region (1.6 Mbp) was deleted in the mouse ES cells (CSCT13 KO). We could establish CSCT13 KO mouse line. Mating between homozygotes gave relatively small number of pups. Moreover, CSCT13 KO mice showed comparatively low rate of homologous recombination during meiosis for the region corresponding to CSCT13. On the other hand, outside of this region showed up-regulation of homologous recombination during meiosis.
This study suggest that CSCT13 might be related with the early embryogenesis and homologous recombination during meiosis.

Research Products

• [Journal Article] GNAS and Kras cooperate to promote murine pancreatic tumorigenesis recapitulating human intraductal papillary mucinous neoplasm. (2016)
  • Author(s): Taki, K., Ohmuraya, M., Tanji, E., Komatsu, H., Hashimoto, D., Semba, K., Araki, K., Kawaguchi, Y., Baba, H. and Furukawa, T.
  • Journal Title: Oncogene Volume: in press Issue: 18 Pages: 1-6
  • DOI: 10.1038/onc.2015.294
  • Peer Reviewed / Open Access
• [Journal Article] The importance of basonuclin 2 in adult mice and its relation to basonuclin 1. (2016)
  • Author(s): Vanhoutteghem A, Delhomme B, Hervé F, Nondier I, Petit JM, Araki M, Araki K, Djian P.
  • Journal Title: Mechanisms of Development Volume: Feb 29 Pages: 1-21
  • DOI: 10.1016/j.mod.2016.02.002
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Severe ocular phenotypes in Rbp4-deficient mice in the C57BL/6 genetic background. (2016)
  • Author(s): Shen, J., Shi, D., Suzuki, T., Xia, Z., Zhang, H., Araki, K., Wakana, S., Takeda, N., Yamamura, K., Jin, S. and Li. Z.
  • Journal Title: Lab. Invest. Volume: in press Issue: 6 Pages: 1-12
  • DOI: 10.1038/labinvest.2016.39
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Rad18 confers hematopoietic progenitor cell DNA damage tolerance independently of the Fanconi Anemia pathway in vivo. (2016)
  • Author(s): Yang, Y., Poe, J. C., Yang, L., Fedoriw, A., Desai, S., Magnuson, T., Li, Z., Fedoriw, Y., Araki, K., Gao, Y., Tateishi, S., Sarantopoulos, S. and Vaziri, C.
  • Journal Title: Nucleic Acids Res. Volume: February 15 Issue: 9 Pages: 1-15
  • DOI: 10.1093/nar/gkw072
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pathophysiological significance of the two-pore domain K(+) channel K2P5.1 in splenic CD4(+)CD25(-) T cell subset from a chemically-induced murine inflammatory bowel disease model. (2015)
  • Author(s): Nakakura S, Matsui M, Sato A, Ishii M, Endo K, Muragishi S, Murase M, Kito H, Niguma H, Kurokawa N, Fujii M, Araki M, Araki K, Ohya S.
  • Journal Title: Frontiers in Physiology Volume: 6 Pages: 299-299
  • DOI: 10.3389/fphys.2015.00299
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] SPINK1 Status in Colorectal Cancer, Impact on Proliferation, and Role in Colitis-associated Cancer. (2015)
  • Author(s): Ida S, Ozaki N, Araki K, Hirashima K, Zaitsu Y, Taki K, Sakamoto Y, Miyamoto Y, Oki E, Morita M, Watanabe M, Maehara Y, Yamamura KI, Baba H Professor, Ohmuraya M.
  • Journal Title: Molecular Cancer Research Volume: - Issue: 7 Pages: 1130-8
  • DOI: 10.1158/1541-7786.mcr-14-0581
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Conformational change in transfer RNA is an early indicator of acute cellular damage. (2014)
  • Author(s): Mishima E, Inoue C, Saigusa D 他33名中13番目
  • Journal Title: J Am Soc Nephrol. Volume: 25 Issue: 10 Pages: 2316-2326
  • DOI: 10.1681/asn.2013091001
  • Peer Reviewed / Open Access
• [Journal Article] NRBF2 Regulates Autophagy and Prevents Liver Injury by Modulating Atg14L-Linked Class III Phosphatidylinositol-3 Kinase Activity. (2014)
  • Author(s): Lu, J., He, L., Behrends, C., Araki, M., Araki, K., Jun Wang, Q., Catanzaro, J. M., Friedman, S. L., Zong, W. X., Fiel, M. I., Li, M. and Yue, Z.
  • Journal Title: Nat. Commun. Volume: 5 Issue: 1 Pages: 3920-3920
  • DOI: 10.1038/ncomms4920
  • Peer Reviewed
• [Journal Article] Disruption of Mbd5 in mice causes neuronal functional deficits and neurobehavioral abnormalities consistent with 2q23.1 microdeletion syndrome. (2014)
  • Author(s): Camarena, V., Cao, L., Abad, C., Abrams, A., Toledo, Y., Araki, K., Araki, M., Walz, K. and Young, J. I.
  • Journal Title: EMBO Mol. Med. Volume: 6 Issue: 8 Pages: 1003-1015
  • DOI: 10.15252/emmm.201404044
  • Peer Reviewed / Open Access
• [Journal Article] Autophagy regulation in the pancreatic acinar cells is independent of epidermal growth factor receptor signaling. (2014)
  • Author(s): Ozaki, N., Fukuchi, Y., Tomiyoshi, S., Uehara1, H., Ida, S., Wang, J., Kimi Araki, K., Sibilia, M., Baba, H., and Yamamura, K. and Ohmuraya, M.
  • Journal Title: Biochem. Biophysic. Res. Comm. Volume: 446 Issue: 1 Pages: 224-230
  • DOI: 10.1016/j.bbrc.2014.02.111
  • Peer Reviewed / Open Access
• [Journal Article] The intramembrane proteases Signal-peptide-peptidase-like 2a and b (SPPL2a/b) have distinct functions in vivo. (2014)
  • Author(s): Schneppenheim, J., Hutti, S., Mentrup, T., Lullmann-Rauch, R., Rothaug, M., Engelke, M., Dittmann, K., Dressel, R., Araki, M., Araki, K., Wienands, J., Fluhrer, R., Saftig, P. and Schroder, B.
  • Journal Title: Mol. Cell. Biol. Volume: 34 (8) Pages: 1398-1411
  • Peer Reviewed
• [Journal Article] Database for exchangeable gene trap clones: Pathway and gene ontology analysis of exchangeable gene trap clone mouse lines. (2014)
  • Author(s): Araki, M., Nakahara, M., Muta, M., Itou, M., Yanai, C., Yamazoe, F., Miyake, M., Morita, A., Araki, M., Okamoto, Y., Nakagata, N., Yoshinobu, K., Yamamura, K. and Araki, K.
  • Journal Title: Dev. Growth Diffr. Volume: 56 (2) Pages: 161-174
  • NAID: 40019987325
  • Peer Reviewed
• [Journal Article] VMAT2 identified as a regulator of late-stage beta-cell differentiation. (2014)
  • Author(s): Sakano, D., Shiraki, N., Kikawa, K., Yamazoe, T., Kataoka, M., Umeda, K., Araki, K., Mao, D., Matsumoto, S., Nakagata, N., Anderson, O., Stainier, D., Endo, F., Kume, K., Uesugi, M. and Kume, S.
  • Journal Title: Nat. Chem. Biol. Volume: 10 (2) Pages: 141-148
  • Peer Reviewed
• [Journal Article] Generation and analysis of serine protease inhibitor kazal type 3-cre driver mice. (2014)
  • Author(s): Sakata, K., Ohmuraya, M., Araki, K., Suzuki, C., Ida, S., Hashimoto, D., Wang, J., Uchiyama, Y., Baba, H. and Yamamura, K.
  • Journal Title: Exp. Anim. Volume: 63 (1) Pages: 45-53
  • NAID: 130003391605
  • Peer Reviewed
• [Journal Article] Interaction of CD99 with its paralog CD99L2 positively regulates CD99L2 trafficking to cell surfaces. (2013)
  • Author(s): Nam, G., Lee, YK., Lee, HY., Ma, MJ., Araki, M., Araki, K., Lee, S., Lee, IS. and Choi, EY.
  • Journal Title: J. Immunol. Volume: 191 (11) Pages: 5730-5742
  • Peer Reviewed
• [Journal Article] TASK-2: a K2P K(+) channel with complex regulation and diverse physiological functions. (2013)
  • Author(s): Cid, LP., Roa-Rojs, HA., Niemeyer, MI., Gonzalez, W., Araki, M., Araki, K. and Sepulveda, FV.
  • Journal Title: Front Physiol. Volume: 29 July Pages: 198-198
  • Peer Reviewed
• [Journal Article] HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment. (2013)
  • Author(s): Kappel, D., Butter, F., Benda, C., Schelbe, M., Draskovic, I., Stevense, M., Novo, CL., Basquin, C., Araki, M., Araki, K., Krastev, DB., Kittler, R., Jessberger, R., Londofio-Vallejo, JA., Mann, M. and Buchholz, F.
  • Journal Title: EMBO J. Volume: 32 (12) Pages: 1681-1701
  • Peer Reviewed
• [Journal Article] Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice. (2013)
  • Author(s): Nakahara, M., Tateyama, H., Araki, M., Nakagata, N., Yamamura, K. and Araki, K.
  • Journal Title: Mamm. Genome Volume: 24 Pages: 228-239
  • Peer Reviewed
• [Journal Article] Ectopic Expression of Ptf1a Induces Spinal Defects, Urogenital Defects, and Anorectal Malformations in Danforth's Short Tail Mice. (2013)
  • Author(s): Semba, K., Araki, K., Matsumoto, K., Suda, H., Ando, T. Sei, A., Mizuta, H., Takagi, K., Nakahara, M., Muta, M., Yamada, G., Nakagata, N., Iida, A., Ikegawa, S., Nakamura, Y., Araki, M., Abe, K. and Yamamura, K
  • Journal Title: Plos Genetics Volume: 9
  • Peer Reviewed
• [Presentation] GIRK2コンデイショナルKOマウスの作成とそのうつ様行動の解析：うつ様行動としての無動におけるGIRK2チャネルの役割 (2016)
  • Author(s): 本多 幾多郎、荒木 喜美、本田 宗吉、高橋 大貴、副田 二三夫、三隅 将1、山村 研一、高濱 和夫
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-03-09
• [Presentation] 細胞死亢進マウスを用いた新たな代償性増殖に関与する因子の同定 (2015)
  • Author(s): 鈴木俊哉、大村谷昌樹、駒沢幸子、三宅早苗、小池正人、内山安夫、荒木喜美、中野裕康
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] lincRNAの生体内機能解析～遺伝子トラップマウスからのアプローチ～ (2015)
  • Author(s): 吉信公美子、中原舞、山村研一、荒木喜美、荒木正健
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] CHF5074 (CSP-1103)はTtrおよびRbp遺伝子座のダブルヒトマウスの血中ヒトTTR 4量体を安定化する (2015)
  • Author(s): 李正花、荒木喜美、山村研一
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Gene-trap mutagenesis is useful for analysis of long intergenic non-coding RNA genes. (2015)
  • Author(s): Nakahara, M., Yoshinobu, K., Ito, H., Furuhata, R., Yamamura, K., Araki, M. and Araki, K.
  • Organizer: the 29th Annual Conference of the International Mammalian Genome Society
  • Place of Presentation: 横浜市開港記念会館（神奈川県横浜市）
  • Year and Date: 2015-11-08
  • Int'l Joint Research
• [Presentation] Correlation of LincRNA-p21 and p53 expression in the LincRNA-p21 gene trap mouse (2015)
  • Author(s): Furuhata, R., Nakahara, M., Ito, H., Yamamura, K., Araki, M. and Araki, K.
  • Organizer: the 29th Annual Conference of the International Mammalian Genome Society
  • Place of Presentation: 横浜市開港記念会館（神奈川県横浜市）
  • Year and Date: 2015-11-08
  • Int'l Joint Research
• [Presentation] Danforth's short tail (Sd) 変異はトランスポゾン挿入によって引き起こされたPtf1a 遺伝子の異所性発現が原因である (2015)
  • Author(s): 荒木喜美、仙波 圭、中原舞、阿部訓也、荒木正健、山村研一
  • Organizer: 日本遺伝学会第87回大会
  • Place of Presentation: 東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2015-09-24
  • Invited
• [Presentation] 染色体特異的にクラスターを形成しているトラップ領域(CSCT13)の解析 (2015)
  • Author(s): 武田伊世、大賀俊範、荒木美幸、岡本頼幸、吉信公美子、荒木喜美、荒木正健
  • Organizer: 第２９回モロシヌス研究会
  • Place of Presentation: かんぽの宿 有馬（兵庫県神戸市）
  • Year and Date: 2015-07-03
• [Presentation] Cre-driverマウスラインの樹立と解析 (2015)
  • Author(s): 荒木美幸、國場訓、森田彩香、岡本頼幸、山村研一、來海葉子、吉信公美子、荒木正健、荒木喜美
  • Organizer: 第２９回モロシヌス研究会
  • Place of Presentation: 神戸
  • Year and Date: 2015-07-03
• [Presentation] 遺伝子トラップマウスを用いたlincRNAの生体内機能解析 (2015)
  • Author(s): 荒木正健、中原舞、柳井千佳、山添史雅、宮家幹子、森田彩香、岡本頼幸、荒木美幸、伊東春香、山村研一、吉信公美子、荒木喜美
  • Organizer: 第62回日本実験動物学会総会
  • Place of Presentation: 京都テルサ（京都府京都市）
  • Year and Date: 2015-05-28
• [Presentation] 遺伝子改変慢性膵炎モデルマウスではオートファジー不全とネクロプトーシスが誘導される (2015)
  • Author(s): 大村谷昌樹、荒木喜美、山村研一
  • Organizer: 第62回日本実験動物学会総会
  • Place of Presentation: 京都テルサ（京都府京都市）
  • Year and Date: 2015-05-28
• [Presentation] CRISPR/Cas9による２本鎖切断・１本鎖節切断を利用した場合のマウスES細胞における相同組換え効率の比較 (2014)
  • Author(s): 荒木喜美、牟田真由美、仙波圭、竹田直樹、仁木大輔、松本健、武田伊世、山村研一、大村谷昌樹、荒木正健
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 可変型遺伝子トラップマウスラインのパスウェイ解析及びジーンオントロジー解析 (2014)
  • Author(s): 荒木正健、中原舞、中潟直己、山村研一、吉信公美子、荒木喜美
  • Organizer: 日本遺伝学会第８６回大会
  • Place of Presentation: 長浜バイオ大学（滋賀県長浜市）
  • Year and Date: 2014-09-17 – 2014-09-19
• [Presentation] 遺伝子トラップマウスを用いたlincRNAの生体内機能解析 (2014)
  • Author(s): 宮家幹子、森田彩香、柳井千佳、山添史雅、中原舞、荒木美幸、岡本頼幸、伊東春香、大西雄一朗、国場訓、山村研一、吉信公美子、荒木正健、荒木喜美
  • Organizer: 第２８回モロシヌス研究会
  • Place of Presentation: 修善寺総合会館（静岡県伊豆市）
  • Year and Date: 2014-06-27 – 2014-06-28
• [Presentation] Establishment and analysis of Cre-driver mouse line (2014)
  • Author(s): 森田彩香、宮家幹子、荒木美幸、岡本頼幸、国場訓、大西雄一朗、山村研一、來海葉子、吉信公美子、荒木正健、荒木喜美
  • Organizer: 第２８回モロシヌス研究会
  • Place of Presentation: 修善寺総合会館（静岡県伊豆市）
  • Year and Date: 2014-06-27 – 2014-06-28
• [Presentation] 可変型遺伝子トラップクローンデータベース[EGTC]の開発と解析 (2014)
  • Author(s): 荒木正健、中原舞、柳井千佳、山添史雅、宮家幹子、森田彩香、荒木美幸、岡本頼幸、中潟直己、吉信公美子、山村研一、荒木喜美
  • Organizer: 第６１回日本実験動物学会総会
  • Place of Presentation: 札幌コンベンションセンター（札幌）
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] 可変型遺伝子トラップクローンを利用したCre-driverマウスの作製 (2013)
  • Author(s): 荒木喜美、森田彩香、宮家幹子、吉信公美子、來海葉子、慶田貴子、古閑成美、中潟直己、村上亜弓、門田雅世、目加田和之、吉木淳、荒木正健
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: つくば国際会議場
• [Presentation] Analysis of gene-trap mouse lines trapping long non-coding RNA genes. (Part 1) (2013)
  • Author(s): 柳井千佳、山添史雅、中原舞、宮家幹子、森田彩香、荒木美幸、岡本頼幸、伊東春香、山村研一、吉信公美子、荒木正健、荒木喜美
  • Organizer: 第27回モロシヌス研究会
  • Place of Presentation: 筑波山 江戸家
• [Presentation] Analysis of gene-trap mouse lines trapping long non-coding RNA genes. (Part 2) (2013)
  • Author(s): 山添史雅、柳井千佳、中原舞、宮家幹子、森田彩香、荒木美幸、岡本頼幸、伊東春香、山村研一、吉信公美子、荒木正健、荒木喜美
  • Organizer: 第27回モロシヌス研究会
  • Place of Presentation: 筑波山 江戸家
• [Presentation] 遺伝子トラップマウスを用いたlincRNAの機能解析 (2013)
  • Author(s): 中原舞、山添史雅、柳井千佳、吉信公美子、山村研一、荒木正健、荒木喜美
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 可変型遺伝子トラップクローンの進展 (2013)
  • Author(s): 吉信公美子、來海葉子、慶田貴子、古閑成美、中原舞、山村研一、荒木喜美、荒木正健
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Remarks] EGTC
  • URL: http://egtc.jp
• [Remarks] EGTC
  • URL: http://egtc.jp