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Functional analysis of chromosome specific clustered trap region (CSCT).

Research Project

Project/Area Number 25640052
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionKumamoto University

Principal Investigator

ARAKI MASATAKE  熊本大学, 生命資源研究・支援センター, 准教授 (80271609)

Co-Investigator(Kenkyū-buntansha) ARAKI Kimi  熊本大学, 生命資源研究・支援センター, 教授 (90211705)
Research Collaborator YOSHINOBU Kumiko  熊本大学, 生命資源研究・支援センター, 助教 (20274730)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords遺伝子トラップ / CSCT / EGTC / ゲノム編集 / 染色体相同組換え / 減数分裂 / クラスター遺伝子 / 染色体特異的 / マウス
Outline of Final Research Achievements

We found new genome element, CSCT (Chromosome Specific Clustered Trap region).
Using CRISPR/Cas9 system, CSCT13 region (1.6 Mbp) was deleted in the mouse ES cells (CSCT13 KO). We could establish CSCT13 KO mouse line. Mating between homozygotes gave relatively small number of pups. Moreover, CSCT13 KO mice showed comparatively low rate of homologous recombination during meiosis for the region corresponding to CSCT13. On the other hand, outside of this region showed up-regulation of homologous recombination during meiosis.
This study suggest that CSCT13 might be related with the early embryogenesis and homologous recombination during meiosis.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (42 results)

All 2016 2015 2014 2013 Other

All Journal Article (19 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 19 results,  Open Access: 7 results,  Acknowledgement Compliant: 2 results) Presentation (21 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (2 results)

  • [Journal Article] GNAS and Kras cooperate to promote murine pancreatic tumorigenesis recapitulating human intraductal papillary mucinous neoplasm.2016

    • Author(s)
      Taki, K., Ohmuraya, M., Tanji, E., Komatsu, H., Hashimoto, D., Semba, K., Araki, K., Kawaguchi, Y., Baba, H. and Furukawa, T.
    • Journal Title

      Oncogene

      Volume: in press Issue: 18 Pages: 1-6

    • DOI

      10.1038/onc.2015.294

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The importance of basonuclin 2 in adult mice and its relation to basonuclin 1.2016

    • Author(s)
      Vanhoutteghem A, Delhomme B, Hervé F, Nondier I, Petit JM, Araki M, Araki K, Djian P.
    • Journal Title

      Mechanisms of Development

      Volume: Feb 29 Pages: 1-21

    • DOI

      10.1016/j.mod.2016.02.002

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Severe ocular phenotypes in Rbp4-deficient mice in the C57BL/6 genetic background.2016

    • Author(s)
      Shen, J., Shi, D., Suzuki, T., Xia, Z., Zhang, H., Araki, K., Wakana, S., Takeda, N., Yamamura, K., Jin, S. and Li. Z.
    • Journal Title

      Lab. Invest.

      Volume: in press Issue: 6 Pages: 1-12

    • DOI

      10.1038/labinvest.2016.39

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Rad18 confers hematopoietic progenitor cell DNA damage tolerance independently of the Fanconi Anemia pathway in vivo.2016

    • Author(s)
      Yang, Y., Poe, J. C., Yang, L., Fedoriw, A., Desai, S., Magnuson, T., Li, Z., Fedoriw, Y., Araki, K., Gao, Y., Tateishi, S., Sarantopoulos, S. and Vaziri, C.
    • Journal Title

      Nucleic Acids Res.

      Volume: February 15 Issue: 9 Pages: 1-15

    • DOI

      10.1093/nar/gkw072

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pathophysiological significance of the two-pore domain K(+) channel K2P5.1 in splenic CD4(+)CD25(-) T cell subset from a chemically-induced murine inflammatory bowel disease model.2015

    • Author(s)
      Nakakura S, Matsui M, Sato A, Ishii M, Endo K, Muragishi S, Murase M, Kito H, Niguma H, Kurokawa N, Fujii M, Araki M, Araki K, Ohya S.
    • Journal Title

      Frontiers in Physiology

      Volume: 6 Pages: 299-299

    • DOI

      10.3389/fphys.2015.00299

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] SPINK1 Status in Colorectal Cancer, Impact on Proliferation, and Role in Colitis-associated Cancer.2015

    • Author(s)
      Ida S, Ozaki N, Araki K, Hirashima K, Zaitsu Y, Taki K, Sakamoto Y, Miyamoto Y, Oki E, Morita M, Watanabe M, Maehara Y, Yamamura KI, Baba H Professor, Ohmuraya M.
    • Journal Title

      Molecular Cancer Research

      Volume: - Issue: 7 Pages: 1130-8

    • DOI

      10.1158/1541-7786.mcr-14-0581

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Conformational change in transfer RNA is an early indicator of acute cellular damage2014

    • Author(s)
      Mishima E, Inoue C, Saigusa D 他33名中13番目
    • Journal Title

      J Am Soc Nephrol.

      Volume: 25 Issue: 10 Pages: 2316

    • DOI

      10.1681/asn.2013091001

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] NRBF2 Regulates Autophagy and Prevents Liver Injury by Modulating Atg14L-Linked Class III Phosphatidylinositol-3 Kinase Activity.2014

    • Author(s)
      Lu, J., He, L., Behrends, C., Araki, M., Araki, K., Jun Wang, Q., Catanzaro, J. M., Friedman, S. L., Zong, W. X., Fiel, M. I., Li, M. and Yue, Z.
    • Journal Title

      Nat. Commun.

      Volume: 5 Issue: 1 Pages: 3920-3920

    • DOI

      10.1038/ncomms4920

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Disruption of Mbd5 in mice causes neuronal functional deficits and neurobehavioral abnormalities consistent with 2q23.1 microdeletion syndrome.2014

    • Author(s)
      Camarena, V., Cao, L., Abad, C., Abrams, A., Toledo, Y., Araki, K., Araki, M., Walz, K. and Young, J. I.
    • Journal Title

      EMBO Mol. Med.

      Volume: 6 Issue: 8 Pages: 1003-1015

    • DOI

      10.15252/emmm.201404044

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autophagy regulation in the pancreatic acinar cells is independent of epidermal growth factor receptor signaling.2014

    • Author(s)
      Ozaki, N., Fukuchi, Y., Tomiyoshi, S., Uehara1, H., Ida, S., Wang, J., Kimi Araki, K., Sibilia, M., Baba, H., and Yamamura, K. and Ohmuraya, M.
    • Journal Title

      Biochem. Biophysic. Res. Comm.

      Volume: 446 Issue: 1 Pages: 224-230

    • DOI

      10.1016/j.bbrc.2014.02.111

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The intramembrane proteases Signal-peptide-peptidase-like 2a and b (SPPL2a/b) have distinct functions in vivo.2014

    • Author(s)
      Schneppenheim, J., Hutti, S., Mentrup, T., Lullmann-Rauch, R., Rothaug, M., Engelke, M., Dittmann, K., Dressel, R., Araki, M., Araki, K., Wienands, J., Fluhrer, R., Saftig, P. and Schroder, B.
    • Journal Title

      Mol. Cell. Biol.

      Volume: 34 (8) Pages: 1398-1411

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Database for exchangeable gene trap clones: Pathway and gene ontology analysis of exchangeable gene trap clone mouse lines.2014

    • Author(s)
      Araki, M., Nakahara, M., Muta, M., Itou, M., Yanai, C., Yamazoe, F., Miyake, M., Morita, A., Araki, M., Okamoto, Y., Nakagata, N., Yoshinobu, K., Yamamura, K. and Araki, K.
    • Journal Title

      Dev. Growth Diffr.

      Volume: 56 (2) Pages: 161-174

    • NAID

      40019987325

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] VMAT2 identified as a regulator of late-stage beta-cell differentiation.2014

    • Author(s)
      Sakano, D., Shiraki, N., Kikawa, K., Yamazoe, T., Kataoka, M., Umeda, K., Araki, K., Mao, D., Matsumoto, S., Nakagata, N., Anderson, O., Stainier, D., Endo, F., Kume, K., Uesugi, M. and Kume, S.
    • Journal Title

      Nat. Chem. Biol.

      Volume: 10 (2) Pages: 141-148

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Generation and analysis of serine protease inhibitor kazal type 3-cre driver mice.2014

    • Author(s)
      Sakata, K., Ohmuraya, M., Araki, K., Suzuki, C., Ida, S., Hashimoto, D., Wang, J., Uchiyama, Y., Baba, H. and Yamamura, K.
    • Journal Title

      Exp. Anim.

      Volume: 63 (1) Pages: 45-53

    • NAID

      130003391605

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Interaction of CD99 with its paralog CD99L2 positively regulates CD99L2 trafficking to cell surfaces.2013

    • Author(s)
      Nam, G., Lee, YK., Lee, HY., Ma, MJ., Araki, M., Araki, K., Lee, S., Lee, IS. and Choi, EY.
    • Journal Title

      J. Immunol.

      Volume: 191 (11) Pages: 5730-5742

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] TASK-2: a K2P K(+) channel with complex regulation and diverse physiological functions.2013

    • Author(s)
      Cid, LP., Roa-Rojs, HA., Niemeyer, MI., Gonzalez, W., Araki, M., Araki, K. and Sepulveda, FV.
    • Journal Title

      Front Physiol.

      Volume: 29 July Pages: 198-198

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment.2013

    • Author(s)
      Kappel, D., Butter, F., Benda, C., Schelbe, M., Draskovic, I., Stevense, M., Novo, CL., Basquin, C., Araki, M., Araki, K., Krastev, DB., Kittler, R., Jessberger, R., Londofio-Vallejo, JA., Mann, M. and Buchholz, F.
    • Journal Title

      EMBO J.

      Volume: 32 (12) Pages: 1681-1701

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice.2013

    • Author(s)
      Nakahara, M., Tateyama, H., Araki, M., Nakagata, N., Yamamura, K. and Araki, K.
    • Journal Title

      Mamm. Genome

      Volume: 24 Pages: 228-239

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ectopic Expression of Ptf1a Induces Spinal Defects, Urogenital Defects, and Anorectal Malformations in Danforth's Short Tail Mice.2013

    • Author(s)
      Semba, K., Araki, K., Matsumoto, K., Suda, H., Ando, T. Sei, A., Mizuta, H., Takagi, K., Nakahara, M., Muta, M., Yamada, G., Nakagata, N., Iida, A., Ikegawa, S., Nakamura, Y., Araki, M., Abe, K. and Yamamura, K
    • Journal Title

      Plos Genetics

      Volume: 9

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] GIRK2コンデイショナルKOマウスの作成とそのうつ様行動の解析:うつ様行動としての無動におけるGIRK2チャネルの役割2016

    • Author(s)
      本多 幾多郎、荒木 喜美、本田 宗吉、高橋 大貴、副田 二三夫、三隅 将1、山村 研一、高濱 和夫
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞死亢進マウスを用いた新たな代償性増殖に関与する因子の同定2015

    • Author(s)
      鈴木俊哉、大村谷昌樹、駒沢幸子、三宅早苗、小池正人、内山安夫、荒木喜美、中野裕康
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] lincRNAの生体内機能解析~遺伝子トラップマウスからのアプローチ~2015

    • Author(s)
      吉信公美子、中原舞、山村研一、荒木喜美、荒木正健
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] CHF5074 (CSP-1103)はTtrおよびRbp遺伝子座のダブルヒトマウスの血中ヒトTTR 4量体を安定化する2015

    • Author(s)
      李正花、荒木喜美、山村研一
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Gene-trap mutagenesis is useful for analysis of long intergenic non-coding RNA genes.2015

    • Author(s)
      Nakahara, M., Yoshinobu, K., Ito, H., Furuhata, R., Yamamura, K., Araki, M. and Araki, K.
    • Organizer
      the 29th Annual Conference of the International Mammalian Genome Society
    • Place of Presentation
      横浜市開港記念会館(神奈川県横浜市)
    • Year and Date
      2015-11-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Correlation of LincRNA-p21 and p53 expression in the LincRNA-p21 gene trap mouse2015

    • Author(s)
      Furuhata, R., Nakahara, M., Ito, H., Yamamura, K., Araki, M. and Araki, K.
    • Organizer
      the 29th Annual Conference of the International Mammalian Genome Society
    • Place of Presentation
      横浜市開港記念会館(神奈川県横浜市)
    • Year and Date
      2015-11-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Danforth's short tail (Sd) 変異はトランスポゾン挿入によって引き起こされたPtf1a 遺伝子の異所性発現が原因である2015

    • Author(s)
      荒木喜美、仙波 圭、中原舞、阿部訓也、荒木正健、山村研一
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      東北大学川内北キャンパス(宮城県仙台市)
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 染色体特異的にクラスターを形成しているトラップ領域(CSCT13)の解析2015

    • Author(s)
      武田伊世、大賀俊範、荒木美幸、岡本頼幸、吉信公美子、荒木喜美、荒木正健
    • Organizer
      第29回モロシヌス研究会
    • Place of Presentation
      かんぽの宿 有馬(兵庫県神戸市)
    • Year and Date
      2015-07-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] Cre-driverマウスラインの樹立と解析2015

    • Author(s)
      荒木美幸、國場訓、森田彩香、岡本頼幸、山村研一、來海葉子、吉信公美子、荒木正健、荒木喜美
    • Organizer
      第29回モロシヌス研究会
    • Place of Presentation
      神戸
    • Year and Date
      2015-07-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 遺伝子トラップマウスを用いたlincRNAの生体内機能解析2015

    • Author(s)
      荒木正健、中原舞、柳井千佳、山添史雅、宮家幹子、森田彩香、岡本頼幸、荒木美幸、伊東春香、山村研一、吉信公美子、荒木喜美
    • Organizer
      第62回日本実験動物学会総会
    • Place of Presentation
      京都テルサ(京都府京都市)
    • Year and Date
      2015-05-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 遺伝子改変慢性膵炎モデルマウスではオートファジー不全とネクロプトーシスが誘導される2015

    • Author(s)
      大村谷昌樹、荒木喜美、山村研一
    • Organizer
      第62回日本実験動物学会総会
    • Place of Presentation
      京都テルサ(京都府京都市)
    • Year and Date
      2015-05-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] CRISPR/Cas9による2本鎖切断・1本鎖節切断を利用した場合のマウスES細胞における相同組換え効率の比較2014

    • Author(s)
      荒木喜美、牟田真由美、仙波圭、竹田直樹、仁木大輔、松本健、武田伊世、山村研一、大村谷昌樹、荒木正健
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 可変型遺伝子トラップマウスラインのパスウェイ解析及びジーンオントロジー解析2014

    • Author(s)
      荒木正健、中原舞、中潟直己、山村研一、吉信公美子、荒木喜美
    • Organizer
      日本遺伝学会第86回大会
    • Place of Presentation
      長浜バイオ大学(滋賀県長浜市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 遺伝子トラップマウスを用いたlincRNAの生体内機能解析2014

    • Author(s)
      宮家幹子、森田彩香、柳井千佳、山添史雅、中原舞、荒木美幸、岡本頼幸、伊東春香、大西雄一朗、国場訓、山村研一、吉信公美子、荒木正健、荒木喜美
    • Organizer
      第28回モロシヌス研究会
    • Place of Presentation
      修善寺総合会館(静岡県伊豆市)
    • Year and Date
      2014-06-27 – 2014-06-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Establishment and analysis of Cre-driver mouse line2014

    • Author(s)
      森田彩香、宮家幹子、荒木美幸、岡本頼幸、国場訓、大西雄一朗、山村研一、來海葉子、吉信公美子、荒木正健、荒木喜美
    • Organizer
      第28回モロシヌス研究会
    • Place of Presentation
      修善寺総合会館(静岡県伊豆市)
    • Year and Date
      2014-06-27 – 2014-06-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 可変型遺伝子トラップクローンデータベース[EGTC]の開発と解析2014

    • Author(s)
      荒木正健、中原舞、柳井千佳、山添史雅、宮家幹子、森田彩香、荒木美幸、岡本頼幸、中潟直己、吉信公美子、山村研一、荒木喜美
    • Organizer
      第61回日本実験動物学会総会
    • Place of Presentation
      札幌コンベンションセンター(札幌)
    • Year and Date
      2014-05-15 – 2014-05-17
    • Related Report
      2014 Research-status Report
  • [Presentation] 可変型遺伝子トラップクローンを利用したCre-driverマウスの作製2013

    • Author(s)
      荒木喜美、森田彩香、宮家幹子、吉信公美子、來海葉子、慶田貴子、古閑成美、中潟直己、村上亜弓、門田雅世、目加田和之、吉木淳、荒木正健
    • Organizer
      第60回日本実験動物学会総会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] Analysis of gene-trap mouse lines trapping long non-coding RNA genes. (Part 1)2013

    • Author(s)
      柳井千佳、山添史雅、中原舞、宮家幹子、森田彩香、荒木美幸、岡本頼幸、伊東春香、山村研一、吉信公美子、荒木正健、荒木喜美
    • Organizer
      第27回モロシヌス研究会
    • Place of Presentation
      筑波山 江戸家
    • Related Report
      2013 Research-status Report
  • [Presentation] Analysis of gene-trap mouse lines trapping long non-coding RNA genes. (Part 2)2013

    • Author(s)
      山添史雅、柳井千佳、中原舞、宮家幹子、森田彩香、荒木美幸、岡本頼幸、伊東春香、山村研一、吉信公美子、荒木正健、荒木喜美
    • Organizer
      第27回モロシヌス研究会
    • Place of Presentation
      筑波山 江戸家
    • Related Report
      2013 Research-status Report
  • [Presentation] 遺伝子トラップマウスを用いたlincRNAの機能解析2013

    • Author(s)
      中原舞、山添史雅、柳井千佳、吉信公美子、山村研一、荒木正健、荒木喜美
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Research-status Report
  • [Presentation] 可変型遺伝子トラップクローンの進展2013

    • Author(s)
      吉信公美子、來海葉子、慶田貴子、古閑成美、中原舞、山村研一、荒木喜美、荒木正健
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Research-status Report
  • [Remarks] EGTC

    • URL

      http://egtc.jp

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
  • [Remarks] EGTC

    • URL

      http://egtc.jp

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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