| Project/Area Number |
25640055
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Central Institute for Experimental Animals |
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Principal Investigator |
SUEMIZU Hiroshi 公益財団法人実験動物中央研究所, その他部局等, 研究員 (40332209)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 肝再生 / 肝幹細胞 / ヒト化マウス / FucciTgマウス / Tg-Fucci-S/G2/M-Greenマウス |
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| Outline of Final Research Achievements |
The purpose of this study is to identify the genuine liver stem cells that have the ability to reconstitute a liver, and is to create artificial cell with equivalent capabilities to liver stem cells. We established a simple method for inducing direct hyperplasia and compensatory regeneration of hepatocytes by administration of thioacetamide and ganciclovir to HSVtk transgenic mice, respectively. The future challenge is to establish a method for collecting proliferative liver cells, which appeared in both regenerative patterns. We identified 22 genes involved in growth promotion, and 181 genes involved in hepatic maturation by analyzing a cryopreserved human hepatocytes that are well engrafted in injured liver of TK-NOG mice. On the basis of these findings, we will advance in the exploration of “genuine liver stem cells” and the development of “humanized-liver mice model”.
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