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Genomic/Epigenomic analyses and investigation for pancreatic CSCs with visualizing system

Research Project

Project/Area Number 25640060
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Tumor biology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Ito Hiromitsu  東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (80645474)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords癌幹細胞 / 浸潤・転移 / エピゲノム / 膵臓癌 / 転移 / DCLK-1
Outline of Final Research Achievements

Patients with pancreatic cancer typically develop tumor invasion and metastasis in the early stage. We previously examined the proteasome activity of CSCs and constructed a real-time visualization system for human pancreatic CSCs. In the present study, we found that CSCs were highly metastatic and dominantly localized at the invading tumor margins in a liver metastasis model. Microarray and siRNA screening assays showed that DCLK1 was predominantly expressed with histone modification in pancreatic CSCs with invasive and metastatic potential. Overexpression of DCLK1 led to amoeboid morphology, which promotes the migration of pancreatic cancer cells. Knockdown of DCLK1 profoundly suppressed in vivo liver metastasis of pancreatic CSCs. With clinical samples, our studies revealed that DCLK1 may be a promising epigenetic and therapeutic target in human pancreatic cancer.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (2 results)

All 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results)

  • [Journal Article] Dominant Expression of DCLK1 in Human Pancreatic Cancer Stem Cells Accelerates Tumor Invasion and Metastasis.2016

    • Author(s)
      Ito H, Tanaka S, Akiyama Y, Shimada S, Adikrisna R, Matsumura S, Aihara A, Mitsunori Y, Ban D, Ochiai T, Kudo A, Arii S, Yamaoka S, Tanabe M.
    • Journal Title

      Plos One

      Volume: - Issue: 1 Pages: e0146564-e0146564

    • DOI

      10.1371/journal.pone.0146564

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ヒト膵癌幹細胞可視化システムを用いた転移メカニズムの解析と標的遺伝子の同定2016

    • Author(s)
      伊藤浩光
    • Organizer
      第116回日本外科学会定期学術集会
    • Place of Presentation
      大阪国際会議場(大阪府・大阪市)
    • Year and Date
      2016-04-14
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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