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Chromothripsis-like pattern in cancer-cell genome after irradiation by a focused vertical micro-beam system, SPICE

Research Project

Project/Area Number 25640062
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Tumor biology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

INAZAWA Johji  東京医科歯科大学, 難治疾患研究所, 教授 (30193551)

Co-Investigator(Renkei-kenkyūsha) INOUE Jun  東京医科歯科大学, 難治疾患研究所, 講師 (50568326)
TANIMOTO Kosuke  東京医科歯科大学, 難治疾患研究所, 助教 (60611613)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsChromothripsis / 染色体粉砕 / ゲノム再構成 / ゲノム不安定性 / マイクロビーム照射装置 / DNA二重鎖切断 / 口腔がん / 次世代シークエンサー / HOC313 / 次世代型シークエンサー / がん / 全ゲノムシークエンス / chromothripsis
Outline of Final Research Achievements

Chromothripsis is a catastrophic cellular event in cancer, in which chromosomes undergo localized massive copy number alterations and rearrangements. However the causes and consequences of chromothripsis are almost unclear. We investigated whether chromothripsis was artificially induced by irradiation with the Single Particle Irradiation system to Cell (SPICE), a focused vertical micro-beam system designed to irradiate the nuclei of adhesive cells. Multiple chromosomal alterations involved in chromosome 7 with a complex translocation, suggesting chromothripsis to be induced in part by irradiation.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (39 results)

All 2015 2014 2013 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (24 results) Remarks (3 results)

  • [Journal Article] Integrated genomic and functional analyses reveal glyoxalase I as a novel metabolic oncogene in human gastric cancer.2014

    • Author(s)
      Hosoda F, Inazawa J, et al.
    • Journal Title

      Oncogene.

      Volume: in press Issue: 9 Pages: 1196-206

    • DOI

      10.1038/onc.2014.57

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] NF-κB Inducing Kinase, a Central Signaling Component of the Non-Canonical Pathway of NF-κB, Contributes to Ovarian Cancer Progression.2014

    • Author(s)
      Uno M, Saitoh Y, Mochida K, Tsuruyama E, Kiyono T, Imoto I, Inazawa J, Yuasa Y, Kubota T, Yamaoka S.
    • Journal Title

      PLoS One

      Volume: 9(2) Issue: 2 Pages: e88347-e88347

    • DOI

      10.1371/journal.pone.0088347

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genetic variants in C5 and poor response to eculizumab.2014

    • Author(s)
      Nishimura J, Inazawa J, et al.
    • Journal Title

      N Engl J Med.

      Volume: 370 Issue: 7 Pages: 632-9

    • DOI

      10.1056/nejmoa1311084

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] γ-Glutamylcyclotransferase as a novel immunohistochemical biomarker for the malignancy of esophageal squamous tumors.2014

    • Author(s)
      Takemura K, Inazawa J, et al.
    • Journal Title

      Hum Pathol.

      Volume: 45 Issue: 2 Pages: 331-41

    • DOI

      10.1016/j.humpath.2013.09.009

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Significance of Akt activation and AKT gene increases in soft tissue tumors.2014

    • Author(s)
      Dobashi Y, Inazawa J, et al.
    • Journal Title

      Hum Pathol.

      Volume: 45 Issue: 1 Pages: 127-36

    • DOI

      10.1016/j.humpath.2013.06.024

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The impact of miRNA-based molecular diagnostics and treatment of NRF2-stabilized tumors.2014

    • Author(s)
      Yamamoto S, Inazawa J, et al.
    • Journal Title

      Mol Cancer Res.

      Volume: 12 Issue: 1 Pages: 58-68

    • DOI

      10.1158/1541-7786.mcr-13-0246-t

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genome-wide association study of breast cancer in the Japanese population.2013

    • Author(s)
      Low SK, Inazawa J, et al.
    • Journal Title

      PLoS One.

      Volume: 8 Issue: 10 Pages: e76463-e76463

    • DOI

      10.1371/journal.pone.0076463

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Significance of GSTP1 for predicting the prognosis and chemotherapeutic efficacy in esophageal squamous cell carcinoma.2013

    • Author(s)
      Yamamoto Y, Inazawa J, et al.
    • Journal Title

      Oncol Rep.

      Volume: 30 Issue: 4 Pages: 1687-94

    • DOI

      10.3892/or.2013.2606

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] miR-655 is an EMT-suppressive microRNA targeting ZEB1 and TGFBR2.2013

    • Author(s)
      Harazono Y, Inazawa J, et al.
    • Journal Title

      PLoS One.

      Volume: 8 Issue: 5 Pages: e62757-e62757

    • DOI

      10.1371/journal.pone.0062757

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Tumor-suppressive mir-497-195 cluster targets multiple cell-cycle regulators in hepatocellular carcinoma.2013

    • Author(s)
      Furuta M
    • Journal Title

      PLoS One

      Volume: 8 Issue: 3 Pages: e60155-e60155

    • DOI

      10.1371/journal.pone.0060155

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Potential of tumor-suppressive miR-596 targeting LGALS3BP as a therapeutic agent in oral cancer.2013

    • Author(s)
      Endo H, Inazawa J, et al.
    • Journal Title

      Carcinogenesis.

      Volume: 34 Issue: 3 Pages: 560-9

    • DOI

      10.1093/carcin/bgs376

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Esophageal Squamous Cell Carcinoma Developed 11 Years After Allogeneic Bone Marrow Transplantation for Acute Lymphatic Leukemia.2013

    • Author(s)
      Miyawaki Y, Inazawa J, et al.
    • Journal Title

      Jpn J Clin Oncol.

      Volume: 43 Issue: 1 Pages: 69-73

    • DOI

      10.1093/jjco/hys184

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Exploration of mechanisms for chromothripsis by irradiation2015

    • Author(s)
      18.Morishita M. Muramatsu T. Hayashi S. Hirai M. Suto Y. Konishi T. Moriyama K. Inazawa J
    • Organizer
      AACR Annual Meeting
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2015-04-18 – 2015-04-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Pathogenic CNVs and causative gene analysis by SNP arrays as the third screening for 646 patients with intellectual disability and multiple congenital anomalies of unknown etiology.2013

    • Author(s)
      D.T.Uehara、稲澤譲治 他
    • Organizer
      American Society of Human Genetics 63th annual meeting.
    • Place of Presentation
      Boston, USA.
    • Related Report
      2013 Research-status Report
  • [Presentation] Genetic and epigenetic aberrations of autophagy-related genes in human cancers.

    • Author(s)
      稲澤譲治
    • Organizer
      2013 SNUCRI & SNUCH Cancer Symposium.
    • Place of Presentation
      Phooenixisland,Jeju, Korea.
    • Related Report
      2013 Research-status Report
  • [Presentation] Investigation of CASK gene aberrations in 38 patients with severe intellectual disability, microcephaly and disproportionate pontine and cerebellar hypoplasia (MICPCH).

    • Author(s)
      林深、稲澤譲治 他
    • Organizer
      American Society of Human Genetics 63th annual meeting.
    • Place of Presentation
      Boston, USA.
    • Related Report
      2013 Research-status Report
  • [Presentation] 癌治療におけるオートファジー活性の評価.

    • Author(s)
      井上純、稲澤譲治
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜.神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] Eカドヘリン・プロモーター活性を指標としたEMT抑制性microRNAの機能的探索.

    • Author(s)
      小崎健一、稲澤譲治
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜.神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] がん抑制型マイクロRNAの探索.

    • Author(s)
      稲澤譲治
    • Organizer
      日本人類遺伝学会第58回大会
    • Place of Presentation
      江陽グランドホテル.宮城
    • Related Report
      2013 Research-status Report
  • [Presentation] Chromosome 19 amplicon in oral cancer cell line have the potential to promote metastasis via Rho signaling pathway.

    • Author(s)
      村松智輝、稲澤譲治 他
    • Organizer
      平成25年度文部科学省科学研究費補助金・新学術領域研究「がん研究分野の特性等を踏まえた支援活動」. がん若手研究者ワークショップ
    • Place of Presentation
      蓼科グランドホテル滝の湯.長野
    • Related Report
      2013 Research-status Report
  • [Presentation] がん抑制型マイクロRNAの探索.

    • Author(s)
      稲澤譲治
    • Organizer
      第9回トランスレーショナルリサーチワークショップ
    • Place of Presentation
      都市センターホテル.東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 癌ゲノム研究と個別化医療.

    • Author(s)
      稲澤譲治
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜.神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] がんと遺伝疾患のゲノム・エピゲノム解析.

    • Author(s)
      稲澤譲治
    • Organizer
      第439回日本皮膚科学会大阪地方会/第20回小林浩記念講演
    • Place of Presentation
      朝日生命ホール.大阪
    • Related Report
      2013 Research-status Report
  • [Presentation] 口腔・食道扁平上皮癌の統合的ゲノム・エピゲノム解析.

    • Author(s)
      稲澤譲治
    • Organizer
      第37回日本頭頸部癌学会
    • Place of Presentation
      京王プラザホテル.東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 食道扁平上皮癌におけるリンパ節転移予測マーカー同定を目指した網羅的DNAメチル化探索.

    • Author(s)
      永田啓明、稲澤譲治 他
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜.神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] オートファジー障害を持つ癌細胞における化合物スクリーニング.

    • Author(s)
      岩舘怜子、稲澤譲治 他
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜.神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] NRF2活性化癌に対するMicroRNAを基盤とした診断・治療.

    • Author(s)
      山本信祐、稲澤譲治 他
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜.神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] 食道癌におけるLAPTM5遺伝子の発現低下.

    • Author(s)
      Michelle Nuylan、稲澤譲治 他
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜.神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] Inherent resistance to eculizumab in PNH.

    • Author(s)
      山本正樹、稲澤譲治 他
    • Organizer
      第75回日本血液学会学術総会
    • Place of Presentation
      さっぽろ芸文館.北海道
    • Related Report
      2013 Research-status Report
  • [Presentation] 口腔扁平上皮癌患者のゲノム構造異常解析による治療抵抗性予測.

    • Author(s)
      森田圭一、稲澤譲治 他
    • Organizer
      第51回日本癌治療学会学術集会
    • Place of Presentation
      国立京都国際会館.京都
    • Related Report
      2013 Research-status Report
  • [Presentation] 口腔扁平上皮癌患者のゲノム構造異常解析による治療抵抗性予測.

    • Author(s)
      森田圭一、稲澤譲治 他
    • Organizer
      第37回日本頭頸部癌学会
    • Place of Presentation
      京王プラザホテル.東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 骨軟部腫瘍におけるAktの活性化と遺伝子増加の意義.

    • Author(s)
      土橋洋、稲澤譲治 他
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜.神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] 口腔がん細胞株に認められた19番染色体の増幅領域に座位する遺伝子は、Rhoシグナル経路を介して転移に寄与している可能性がある.

    • Author(s)
      村松智輝、稲澤譲治 他
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜.神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] 複数癌腫における、MicroRNA-634による細胞死誘導機構.

    • Author(s)
      藤原直人、稲澤譲治 他
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜.神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] 次世代シーケンサーを用いたGorlin症候群患者におけるhedgehog pathwayのゲノム解析.

    • Author(s)
      森田圭一、稲澤譲治 他
    • Organizer
      第58回日本口腔外科学会学術総会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡.福岡
    • Related Report
      2013 Research-status Report
  • [Presentation] 次世代シーケンサーを用いたGorlin症候群患者におけるhedgehog pathwayのゲノム解析.

    • Author(s)
      森田圭一、稲澤譲治 他
    • Organizer
      日本人類遺伝学会第58回大会
    • Place of Presentation
      江陽グランドホテル.宮城
    • Related Report
      2013 Research-status Report
  • [Remarks] CGH Data Base

    • URL

      http://www.cghtmd.jp/CGHDatabase/index_j.jsp

    • Related Report
      2014 Annual Research Report
  • [Remarks] MCG CNV database

    • URL

      http://www.cghtmd.jp/CNVDatabase/top.action

    • Related Report
      2014 Annual Research Report
  • [Remarks] 東京医科歯科大学難治疾患研究所分子細胞遺伝ホームページ

    • URL

      http://www.tmd.ac.jp/mri/cgen/framepage.htm

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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