Establishment of breast PDX mouse models with high metastatic potential
| Project/Area Number |
25640069
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Juntendo University |
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Principal Investigator |
orimo akira 順天堂大学, 医学(系)研究科(研究院), 准教授 (70275866)
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| Co-Investigator(Kenkyū-buntansha) |
HORIMOTO Yoshiya 順天堂大学, 医学部, 准教授 (40424246)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 乳癌 / PDXｓ / 癌微小環境 / CAFｓ / 患者乳癌 / ＣＡＦｓ / ＰＤＸモデル / 転移 / 高度免疫不全マウス / PDX model / 癌転移 / 癌内線維芽細胞 |
|---|
| Outline of Final Research Achievements |
Human primary breast tumors were dissected from 10 breast cancer patients without any treatment in this study. Small tumor fragments were implanted orthotopically into NOG immunodeficient mice. As the result, one specimen generated an advanced tumor in 1st NOG mice, however, the tumor was not transplantable in 2nd NOG mice. The rest of 9 specimens were never grown in recipient mice for periods of 6 months. The previous report indicated very low rate (2.5%) of implantation of human breast tissues in immunodeficient mice, when 423 specimens of ER-positive human breast cancers were examined. As recent report also suggested the technical benefit of use of the intraductal injection of cancer cells but not the injection into mammary fad pad with estrogen treatment to increase implantation of cancer cells. Further study requires for overcoming the low implantation ratio and for developing the PDX model efficiently.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Cartilage oligomeric matrix protein contributes to the development and metastasis of breast cancer.2016
Author(s)
Englund E, Bartoschek M, Reitsma B, Jacobsson L, Escudero-Esparza A, Orimo A, Leandersson K, Hagerling C, Aspberg A, Storm P, Okroj M, Mulder H, Jirström K, Pietras K, Blom AM.
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Journal Title
Oncogene
Volume: 11
Pages: 10-10
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] The human complement inhibitor Sushi Domain-Containing Protein 4 (SUSD4) expression in tumor cells and CD8+ T cells is associated with better prognosis of breast cancer patients.2015
Author(s)
Englund E, Reitsma B, King BC, Escudero-Esparza A, Owen S, Orimo A, Okroj M, Anagnostaki L, Jiang WG, Jirström K, Blom AM.
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Journal Title
BMC Cancer,
Volume: 19
Pages: 737-737
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Carcinoma-associated fibroblasts convert barely metastatic human breast cancer cells to highly metastatic cancer cells.2014
Author(s)
Matsumura Y., Ito Y., Wali N., Mogushi K., Terao Y., Takeda S., Okumura K., Takeda K., Hino O., and Orimo A.,
Organizer
第73回日本癌学会学術総会
Place of Presentation
横浜
Year and Date
2014-09-26
Related Report
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[Book] 呼吸2016
Author(s)
伊藤匠、松村優子、伊藤恭彦、折茂彰
Total Pages
141
Publisher
呼吸研究
Related Report
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[Book] 呼吸器内科2015
Author(s)
折茂彰, 松村優子，伊藤恭彦，十合晋作
Total Pages
87
Publisher
科学評論社
Related Report
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