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Establishment of breast PDX mouse models with high metastatic potential

Research Project

Project/Area Number 25640069
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Tumor biology
Research InstitutionJuntendo University

Principal Investigator

orimo akira  順天堂大学, 医学(系)研究科(研究院), 准教授 (70275866)

Co-Investigator(Kenkyū-buntansha) HORIMOTO Yoshiya  順天堂大学, 医学部, 准教授 (40424246)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords乳癌 / PDXs / 癌微小環境 / CAFs / 患者乳癌 / CAFs / PDXモデル / 転移 / 高度免疫不全マウス / PDX model / 癌転移 / 癌内線維芽細胞
Outline of Final Research Achievements

Human primary breast tumors were dissected from 10 breast cancer patients without any treatment in this study. Small tumor fragments were implanted orthotopically into NOG immunodeficient mice. As the result, one specimen generated an advanced tumor in 1st NOG mice, however, the tumor was not transplantable in 2nd NOG mice. The rest of 9 specimens were never grown in recipient mice for periods of 6 months. The previous report indicated very low rate (2.5%) of implantation of human breast tissues in immunodeficient mice, when 423 specimens of ER-positive human breast cancers were examined. As recent report also suggested the technical benefit of use of the intraductal injection of cancer cells but not the injection into mammary fad pad with estrogen treatment to increase implantation of cancer cells. Further study requires for overcoming the low implantation ratio and for developing the PDX model efficiently.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (24 results)

All 2016 2015 2014 2013 Other

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 4 results) Presentation (8 results) (of which Invited: 6 results) Book (5 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Cartilage oligomeric matrix protein contributes to the development and metastasis of breast cancer.2016

    • Author(s)
      Englund E, Bartoschek M, Reitsma B, Jacobsson L, Escudero-Esparza A, Orimo A, Leandersson K, Hagerling C, Aspberg A, Storm P, Okroj M, Mulder H, Jirström K, Pietras K, Blom AM.
    • Journal Title

      Oncogene

      Volume: 11 Pages: 10-10

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The human complement inhibitor Sushi Domain-Containing Protein 4 (SUSD4) expression in tumor cells and CD8+ T cells is associated with better prognosis of breast cancer patients.2015

    • Author(s)
      Englund E, Reitsma B, King BC, Escudero-Esparza A, Owen S, Orimo A, Okroj M, Anagnostaki L, Jiang WG, Jirström K, Blom AM.
    • Journal Title

      BMC Cancer,

      Volume: 19 Pages: 737-737

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The biology of tumour metastasis2014

    • Author(s)
      Orimo A
    • Journal Title

      Juntendo Medical Journal

      Volume: 60 Pages: 56-62

    • NAID

      130004684317

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] がん細胞の浸潤・転移を促進するがん微小環境:がん内線維芽細胞を例に2014

    • Author(s)
      松村優子,伊藤恭彦,折茂彰
    • Journal Title

      細胞工学

      Volume: 33, 6 Pages: 609-12

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Carcinoma Associated Fibroblasts.2013

    • Author(s)
      Polanska, Y. * and Orimo A
    • Journal Title

      Journal of Cellular Physiology,

      Volume: 228, Pages: 1651-7

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Carcinoma-associated fibroblasts (CAFs) are a promising therapeutic target.2013

    • Author(s)
      Togo, S.*, Polanska, Y., Horimoto, Y and Orimo, A
    • Journal Title

      Cancers,

      Volume: 5, Pages: 149-169

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Carcinoma-associated fibroblasts confer adherent epithelial cell traits in breast cancer cells to promote metastasis2015

    • Author(s)
      Orimo A
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 癌細胞と癌内線維芽細胞の共進化および癌悪性化2015

    • Author(s)
      Orimo A
    • Organizer
      第30回発癌病理研究会
    • Place of Presentation
      小豆島
    • Year and Date
      2015-08-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 肺線維症研究の最前線 線維化と癌悪性化2015

    • Author(s)
      折茂彰
    • Organizer
      第12回肺研究フォーラム21
    • Place of Presentation
      東京
    • Year and Date
      2015-04-18
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Evolution of human breast carcinoma cells with stromal myofibroblasts to propagate.2014

    • Author(s)
      折茂彰
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] The subsequent epithelial-mesenchymal and mesenchymal-epithelial transition model barely depicts colon cancer metastasis2014

    • Author(s)
      Okazawa Y., Mizukoshi K., Komiyama H., Fujii T., Goto M., Habu S., Hino O., Sakamoto K., and Orimo A.
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Carcinoma-associated fibroblasts convert barely metastatic human breast cancer cells to highly metastatic cancer cells.2014

    • Author(s)
      Matsumura Y., Ito Y., Wali N., Mogushi K., Terao Y., Takeda S., Okumura K., Takeda K., Hino O., and Orimo A.,
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 間質性肺炎に合併した肺癌の現状と治療戦略- 炎症や線維化2014

    • Author(s)
      折茂彰
    • Organizer
      第54回日本呼吸器学会
    • Place of Presentation
      大坂
    • Year and Date
      2014-04-25
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Carcinoma-associated fibroblasts confer metastatic abilities into apposed human mammary carcinoma cells2013

    • Author(s)
      Akira Orimo, Yasuhiko Ito, Urszula M Polanska, Kazuyoshi Takeda, Ko Okumura, Okio Hino and Yoshiya Horimoto.
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] 実験医学2016

    • Author(s)
      折茂彰
    • Total Pages
      141
    • Publisher
      羊土社
    • Related Report
      2015 Annual Research Report
  • [Book] 呼吸2016

    • Author(s)
      伊藤匠、松村優子、伊藤恭彦、折茂彰
    • Total Pages
      141
    • Publisher
      呼吸研究
    • Related Report
      2015 Annual Research Report
  • [Book] 実験医学2015

    • Author(s)
      折茂彰, 松村優子,本多一貴, Nadila Wali
    • Total Pages
      210
    • Publisher
      羊土社
    • Related Report
      2015 Annual Research Report
  • [Book] 呼吸器内科2015

    • Author(s)
      折茂彰, 松村優子,伊藤恭彦,十合晋作
    • Total Pages
      87
    • Publisher
      科学評論社
    • Related Report
      2015 Annual Research Report
  • [Book] 肺の損傷と発癌機序「Annual Review呼吸器20152015

    • Author(s)
      谷口源太郎、折茂彰
    • Total Pages
      258
    • Publisher
      中外医学社
    • Related Report
      2015 Annual Research Report
  • [Remarks] 文部科学省科学研究費新学術領域―がん研究分野の特性等を踏まえた支援活動

    • URL

      http://ganshien.umin.jp/research/main/orimo/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 癌支援研究者紹介 折茂彰

    • URL

      http://ganshien.umin.jp/research/main/orimo/index.html

    • Related Report
      2014 Research-status Report
  • [Remarks] 癌支援研究紹介

    • URL

      http://ganshien.umin.jp/research/main/orimo/03.html

    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 強転移性ヒト癌細胞株2013

    • Inventor(s)
      折茂 彰
    • Industrial Property Rights Holder
      折茂 彰
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-08-30
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 早期癌転移検出法および新規癌転移抑制薬のスクリーニング法2013

    • Inventor(s)
      折茂 彰 他3名
    • Industrial Property Rights Holder
      折茂 彰 他3名
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-09-12
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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