Fluorescent live-imaging of the switching of pyruvate kinase isoforms during tumorigenesis
| Project/Area Number |
25640072
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Miyagi Prefectural Hospital Organization Miyagi Cancer Center |
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Principal Investigator |
ITO SHIGEMI 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 特任研究員 (80600006)
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| Co-Investigator(Kenkyū-buntansha) |
TANUMA Nobuhiro 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 主任研究員 (40333645)
SATO Ikuro 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), ティッシュバンクセンター, センター長 (50225918)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 代謝 / 解糖系 / ワールブルグ効果 / PKM / スプライシング |
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| Outline of Final Research Achievements |
Pyruvate kinase M (PKM) exists as two isoforms, M1 and M2, generated by alternative splicing. Expression of these isoforms switches from M1- to M2-type during tumorigenesis so that normal differentiated and proliferating/tumor cells express M1 and M2, respectively. In this study, a reporter-gene system, enabling us to visualize PKM-switch by cell-autonomous fluorescence, was developed. Using the reporter-gene, we generated transgenic mice, and examined those for lung tumor model. Unfortunately, any fluorescent signals except for auto-fluorescence were detected in tissues examined including tumor of the Tg-mice. More improvement(s) of the Tg-construct and/or alternative methods for introducing it into the mouse genome might be needed.
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Report
(3 results)
Research Products
(9 results)
