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Fluorescent live-imaging of the switching of pyruvate kinase isoforms during tumorigenesis

Research Project

Project/Area Number 25640072
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Tumor biology
Research InstitutionMiyagi Prefectural Hospital Organization Miyagi Cancer Center

Principal Investigator

ITO SHIGEMI  地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 特任研究員 (80600006)

Co-Investigator(Kenkyū-buntansha) TANUMA Nobuhiro  地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 主任研究員 (40333645)
SATO Ikuro  地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), ティッシュバンクセンター, センター長 (50225918)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords代謝 / 解糖系 / ワールブルグ効果 / PKM / スプライシング
Outline of Final Research Achievements

Pyruvate kinase M (PKM) exists as two isoforms, M1 and M2, generated by alternative splicing. Expression of these isoforms switches from M1- to M2-type during tumorigenesis so that normal differentiated and proliferating/tumor cells express M1 and M2, respectively. In this study, a reporter-gene system, enabling us to visualize PKM-switch by cell-autonomous fluorescence, was developed. Using the reporter-gene, we generated transgenic mice, and examined those for lung tumor model. Unfortunately, any fluorescent signals except for auto-fluorescence were detected in tissues examined including tumor of the Tg-mice. More improvement(s) of the Tg-construct and/or alternative methods for introducing it into the mouse genome might be needed.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (9 results)

All 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (4 results)

  • [Journal Article] Abrogation of protein phosphatase 6 promotes skin carcinogenesis induced by DMBA2015

    • Author(s)
      Hayashi K、ほか20名
    • Journal Title

      Oncogene

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sialidase NEU3 contributes neoplastic potential on colon cancer cells as a key modulator of gangliosides by regulating Wnt signaling.2015

    • Author(s)
      Takahashi K、ほか8名
    • Journal Title

      Int J Cancer

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Circulating miR-483-3p and miR-21 is highly expressed in plasma of pancreatic cancer.2015

    • Author(s)
      Abue M、ほか10名
    • Journal Title

      Int J Oncol

      Volume: 46 Pages: 539-47

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gastric-type endocervical glandular neoplasms associated with aberrant p16 expression and K-RAS gene mutation in Peutz-Jeghers syndrome.2014

    • Author(s)
      Ito S、ほか5名
    • Journal Title

      Pathol Int.

      Volume: 64 Pages: 283-8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role of Pyruvate kinase M2 in transcriptional regulation and epithelial-mesenchymal transition2014

    • Author(s)
      Hamabe A、ほか13名
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 111 Pages: 15526-31

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] Regulation of isoform-expression of pyruvate kinase M (PKM) during cellular differentiation and senescence2014

    • Author(s)
      Nomura M、ほか8名
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Characterization of mice expressing a single isoform of pyruvate kinase M2014

    • Author(s)
      Sakamoto Y、ほか8名
    • Organizer
      第73回 日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 単一のピルビン酸キナーゼMアイソフォームを発現するマウスの解析2014

    • Author(s)
      坂本良美、ほか3名
    • Organizer
      第2回 がんと代謝研究会
    • Place of Presentation
      東京
    • Year and Date
      2014-07-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Generation of knock-in mice to dissect isoform-specific roles for each splicing isoform of pyruvate kinase M in vivo2013

    • Author(s)
      松本祥子、坂本良美、野村美有樹、田中遼大、盛田麻美、伊藤しげみ、椎葉健一、野村栄樹、片倉隆一、山下洋二、佐藤郁郎、渡邊利雄、島礼、田沼延公
    • Organizer
      第36回分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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