Development of methods for isolation of human monoclonal antibodies recognizing cancer stem cells

• Project/Area Number: 25640075
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: University of Toyama
• Principal Investigator: ISOBE Masaharu 富山大学, 大学院理工学研究部(工学), 教授 (70211050)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 大腸がん / がん幹細胞 / ヒトモノクローナル抗体 / 癌幹細胞

Outline of Final Research Achievements

We have conducted a large-scale isolation of human monoclonal antibodies from patients with colon cancer by using our high-throughput isolation system for monoclonal antibodies from single cells. We have selected antibody clones that were capable of binding with colon cancer cell lines. Those clones were further selected based on the reactivity against colon cancer stem cells identified by cancer stem cell positive markers including CD44v. We have identified candidate antigens for those clones by using immunoprecipitation combined with mass spectrum analysis as well as reactivity against protein arrays.

Research Products

• [Journal Article] Cells of origin of squamous epithelium, dysplasia and cancer in the head and neck region after bone marrow transplantation. (2014)
  • Author(s): Kano Y, Ishii H, Konno M, Yamasaki M, Miyata H, Nishikawa S, Hamabe A, Ogawa H, Takahashi H, Ohta K, Hasegawa S, Tanaka K, Fukusumi T, Otsuka M, Kawamoto K, Haraguchi N, Fujimoto R, Isobe M, Tomita Y, Matsuura N, Takiguchi S, Mori M, Doki Y.
  • Journal Title: Int J Oncol Volume: 44 Issue: 2 Pages: 443-50
  • DOI: 10.3892/ijo.2013.2206
  • Peer Reviewed
• [Journal Article] Possible involvement of Hcn1 ion channel in learning and memory dysfunction in SAMP8 mice (2013)
  • Author(s): Akbor, K. Tomobe, T. Yamada, J. Kim, H. Mano, N. Kurosawa, K. Sasaki, Y. Nomura and M. Isobe
  • Journal Title: Biochem Biophys Res Commun Volume: 441 Issue: 1 Pages: 25-30
  • DOI: 10.1016/j.bbrc.2013.09.145
  • Peer Reviewed
• [Journal Article] Reduced Level of the BCL11B Protein Is Associated with Adult T-Cell Leukemia/Lymphoma (2013)
  • Author(s): Kurosawa, N.
  • Journal Title: PLoS One Volume: 8 Issue: 1 Pages: e55147-e55147
  • DOI: 10.1371/journal.pone.0055147
  • Peer Reviewed
• [Journal Article] Fine Bubble Mixing (FBM) Culture of E. coli: A Highly Cost-effective Middle Scale-size Culture System (2013)
  • Author(s): H. Yasumitsu, H. Tajima, M. Isobe, S. Kutsuna, S. M. Kawsar, Y. Fujii, R. A. Kanaly, Y. Ozeki and E. Yokota
  • Journal Title: Protein Pept Lett Volume: 20 Pages: 213-217
  • Peer Reviewed
• [Presentation] 種々の免疫動物に由来するプラズマ細胞からの1細胞抗体遺伝子スクリーニンク (2014)
  • Author(s): 磯部 正治、黒澤 信幸
  • Organizer: 第66回日本生物工学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-09
  • Invited