Development of novel molecular diagnosis methods of lung cancer by proteomic signature

• Project/Area Number: 25640076
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: Nagoya University
• Principal Investigator: Yanagisawa Kiyoshi 名古屋大学, 医学系研究科, 講師 (20372112)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 肺癌 / プロテオミクス / 分子診断 / 非小細胞肺癌 / バイオマーカー / 悪性胸膜中皮腫 / バイオインフォマティクス / 翻訳語修飾

Outline of Final Research Achievements

We obtained protein expression profiles from both lung cancer and normal lung specimens. Based upon the information as well as clinical information, we conducted bioinformatic analyses. The resulting proteomic signature could accurately predict both disease-free and overall survival. Accordingly, we have discovered a proteomic signature that can distinguish NSCLC patients with a high risk from those with a favorable prognosis.

Research Products

• [Journal Article] TTF-1/NKX2-1 binds to DDB1 and confers replication stress resistance to lung adenocarcinomas. (2016)
  • Author(s): Liu Z, Yanagisawa K, Griesing S, Iwai M, Kano K, Hotta N, Kajino T, Suzuki M, and Takahashi T.
  • Journal Title: Oncogene Volume: 524 Issue: 26 Pages: 1-9
  • DOI: 10.1038/onc.2016.524
  • Peer Reviewed / Open Access
• [Journal Article] Blood-borne miRNA profile-based diagnostic classifier for lung adenocarcinoma. (2016)
  • Author(s): Tai MC, Yanagisawa K, Nakatochi M, Hotta N, Hosono Y, Kawaguchi K, Naito M, Taniguchi H, Wakai K, Yokoi K, Takahashi T.
  • Journal Title: Scientific Report Volume: 6 Issue: 1 Pages: 1-9
  • DOI: 10.1038/srep31389
  • Peer Reviewed / Open Access
• [Journal Article] miR-342-3p regulates MYC transcriptional activity via direct repression of E2F1 in human lung cancer. (2015)
  • Author(s): Tai MC, Kajino T, Nakatochi M, Arima C, Shimada Y, Suzuki M, Miyoshi H, Yatabe Y, Yanagisawa K, Takahashi T.
  • Journal Title: Carcinogenesis Volume: 36 Pages: 1464-1473
  • DOI: 10.1093/carcin/bgv152
  • Peer Reviewed
• [Journal Article] Lung adenocarcinoma subtypes definable by lung development-related miRNA expression profiles in association with clinicopathologic features (2014)
  • Author(s): Arima C, Kajino T, Tamada Y, Imoto S, ShimadaY, Nakatochi, M, Suzuki M, Isomura H, Yatabe Y, Yamaguchi T, Yanagisawa K, Miyano S, Takahashi T
  • Journal Title: Carcinogenesis Volume: 35 Issue: 10 Pages: 2224-2231
  • DOI: 10.1093/carcin/bgu127
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Quantitative proteomic profiling identifies DPYSL3 as pancreatic ductal adenocarcinoma-associated molecule that regulates cell adhesion and migration by stabilization of focal adhesion complex (2013)
  • Author(s): Kawahara T, Hotta N, Ozawa Y, Kato S, Kano K, Yokoyama Y, Nagino M, Takahashi T, Yanagisawa K
  • Journal Title: PLoS ONE Volume: 8 Issue: 12 Pages: e79654-e79654
  • DOI: 10.1371/journal.pone.0079654
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] NKX2-1/TTF-1: an enigmatic oncogene that functions as a double-edged sword for cancer cell survival and progression (2013)
  • Author(s): Yamaguchi T, Hosono Y, Yanagisawa K, Takahashi T
  • Journal Title: Cancer Cell Volume: 23 Issue: 6 Pages: 718-723
  • DOI: 10.1016/j.ccr.2013.04.002
  • Peer Reviewed
• [Presentation] Proteomic identification of potential biomarkers of thoracic tumors (2017)
  • Author(s): Kiyoshi Yanagisawa
  • Organizer: Fourth Annual US Japan Workshop on Cancer Biomarkers in Collaboration with NCI Early Detection Research Network
  • Place of Presentation: Tempe Mission Palms Hotel and Conference Center Tempe, Arizona, USA
  • Int'l Joint Research / Invited
• [Presentation] プロテオミクス解析のがん研究への応用 (2016)
  • Author(s): 柳澤 聖
  • Organizer: 第7回Japan Mesothelioma Interest Group研究会
  • Place of Presentation: KITTE名古屋、愛知県、名古屋市
  • Invited
• [Presentation] Proteomic identification of potential biomarkers of human lung malignancies (2013)
  • Author(s): Kiyoshi Yanagisawa and Takashi Takahashi
  • Organizer: 第７２回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Invited