| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We developed a novel method for comprehensive analysis of structural changes of cancer genome. Our method is based on a mate-pair analysis, which enables us to collect information of both ends of DNA fragments from genome. In conventional mate-pair analysis, there must contain circularized DNAs derived from multiple DNA fragments and those mate-pair can be a cause of false positive data. To solve this problem we developed a novel method, which allows us to extract “right” information of mate-pairs derived exclusively from circularized DNAs of single DNA origin. By this method, we can make a comprehensive analysis of cancer genome in a highly precise manner, and we could obtain several candidate mate-pair information in ATL(adult T-cell leukemia/lymphoma) samples. This method is useful for analysis of structural changes of cancer genome and will provide us information for diagnosis and treatment of cancer.
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