Comprehensive identification of tumor-targeting molecules based on the quantitative proteome analysis

• Project/Area Number: 25640080
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: Jikei University School of Medicine
• Principal Investigator: YOSHIDA Kiyotsugu 東京慈恵会医科大学, 医学部, 教授 (70345312)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 癌 / プロテオーム / 分子標的 / 質量分析 / リン酸化 / プロテーム / 腫瘍マーカー

Outline of Final Research Achievements

To identify tumor-targeting molecules, we have carried out 2DICAL as the quantitative proteome analysis. By using 2DICAL, we have uncovered novel cancer-related genes. We also found their post-translational modifications.

Research Products

• [Journal Article] Engagement of DYRK2 in proper control for cell division. (2015)
  • Author(s): Nihira NT, Yoshida K.
  • Journal Title: Cell Cycle Volume: 14 Issue: 6 Pages: 802-7
  • DOI: 10.1080/15384101.2015.1007751
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mps1 phosphorylation of condensin II controls chromosome condensation at the onset of mitosis. (2014)
  • Author(s): Kagami Y, Nihira K, Wada S, Ono M, Honda M, Yoshida K.
  • Journal Title: J Cell Biol Volume: 205 Issue: 6 Pages: 781-90
  • DOI: 10.1083/jcb.201308172
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Tumor suppressor p53 induces miR-1915 processing to inhibit Bcl-2 in the apoptotic response to DNA damage. (2014)
  • Author(s): Nakazawa K, Dashzeveg N, Yoshida K.
  • Journal Title: FEBS J Volume: 281 Issue: 13 Pages: 2937-44
  • DOI: 10.1111/febs.12831
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Induction of amphiregulin by p53 promotes apoptosis via control of microRNA biogenesis in response to DNA damage (2014)
  • Author(s): Taira N, Yamaguchi T, Kimura J, Lu Z-G, Fukuda S, Higashiyama S, Ono M, Yoshida K
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 111 Issue: 2 Pages: 717-722
  • DOI: 10.1073/pnas.1313675111
  • Peer Reviewed / Open Access
• [Journal Article] Palmdelphin, a novel target of p53 with Ser46 phosphorylation, controls cell death in response to DNA damage. (2014)
  • Author(s): Dashzeveg N, Taira N, Lu ZG, Kimura J, Yoshida K.
  • Journal Title: Cell Death Dis Volume: 5 Issue: 5 Pages: e1221-e1221
  • DOI: 10.1038/cddis.2014.176
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] DYRK2 controls epithelial-mesenchymal transition in breast cancer by degrading Snail (2013)
  • Author(s): Mimoto R, Taira N, Takahashi H, Yamaguchi T, Okabe M, Uchida K, Miki Y, Yoshida K
  • Journal Title: Cancer Lett Volume: 339 Issue: 2 Pages: 214-225
  • DOI: 10.1016/j.canlet.2013.06.005
  • Peer Reviewed
• [Presentation] The phosphorylation of p53 at ser46 is essential to induce cell death through palmedlphin in response to DNA damage. (2015)
  • Author(s): Dashzeveg N, Yoshida K.
  • Organizer: AACR Annual Meeting 2015
  • Place of Presentation: Philadelphia, PA
  • Year and Date: 2015-04-18 – 2015-04-22
• [Presentation] 細胞死及びmicroRNA代謝制御に関わる新規p53関連遺伝子の同定 (2013)
  • Author(s): 平直江 他
  • Organizer: 第７２回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] Mps1/TKは分裂期染色体凝集を制御する (2013)
  • Author(s): 加賀美裕也 他
  • Organizer: 第７２回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] P53 targets isoform of paralemmin protein in a serine 46 specific manner to promote apoptosis (2013)
  • Author(s): Nurmaa DASHZEVEG 他
  • Organizer: 第７２回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] Epoxomicin耐性細胞はDUSP6/MKP3発現消失に伴いZEB1発現を誘導しE-cadherin発現消失を促す (2013)
  • Author(s): 朝倉正 他
  • Organizer: 第７２回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Remarks] 東京慈恵会医科大学生化学講座ホームページ
  • URL: http://jikei-biochem.wix.com/yoshidalab