Differentiation of EpCAM positive exosome by using the ferritin particles displaying EpCAM-targeting peptide.
| Project/Area Number |
25640082
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnostics
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| Research Institution | Japanese Foundation for Cancer Research |
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Principal Investigator |
SHIBA Kiyotaka 公益財団法人がん研究会, その他部局等, その他 (40196415)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | エクソソーム / エキソソーム / 細胞外分泌小胞 / ナノ粒子 / フェリチン / EpCAM / 悪性腫瘍 / がん診断 / EVs / ペプチドアプタマー |
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| Outline of Final Research Achievements |
The peptide aptamer for EpCAM was fused at the N-terminal end of horse L-chain ferritin, to construct the modified ferritin that has the affinity for EpCAM protein. The ability of recognition for EpCAM of this engineered ferritin was confirmed by ELISA, and the conditions for the preparation of inorganic nano-dots containing ferritin were established.
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Report
(3 results)
Research Products
(2 results)
