Development of Therapeutic Bacteria Machine Packaging with the Production System of Anti-Cancer Protein

Research Project

Project/Area Number	25640095
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Tumor therapeutics
Research Institution	Institute of Physical and Chemical Research
Principal Investigator	Hidefumi Mukai 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, ユニットリーダー (60570885)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	がん治療 / 遺伝子改変細菌 / 抗がんタンパク / 癌治療 / 抗癌タンパク / 腫瘍環境応答
Outline of Final Research Achievements	We successfully developed the B. choshinensis highly efficiently secreting murine tumor necrosis factor-alpha (TNF-alpha) and demonstrated its significant antitumor effects against the cultured TNF-sensitive L929 cells and a tendency to inhibit tumor growth in L929 bearing mice by intra-tumor injection. This is the original report showing the antitumor effects by engineered B. choshinensis, indicating that it is one of the potential bio-machines for synthetic biology and applied microbiology focusing on cancer therapy.

Report

(4 results)