Multiple functions of histone demethylases
| Project/Area Number |
25640098
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Inagaki Takeshi 東京大学, 先端科学技術研究センター, 特任准教授 (10507825)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ヒストン修飾酵素 / ヒストン脱メチル化酵素 / ＪＭＪＤ１Ａ / 複合体形成 / ＨＤＡＣ / 転写 / ＦＢＸＬ１０ / クロマチン構造 / ヒストン / JMJD1A / JMJD1B / エピゲノム / 脂肪細胞分化 / SIN3A / ヒストン脱アセチル化 / ＳＩＮ３Ａ |
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| Outline of Final Research Achievements |
JmjC domain is a demethylase signature motif and most of JmjC-containing-proteins work as histone demethylase. However, in this study, we demonstrated that JmjC-containing proteins also play non-enzymatic roles. Shotgun proteomic analysis using HeLa cells showed that JMJD1A binds to SIN3A complex including ARID1A and histone deacetylase (HDAC) 1. HDAC activity was induced by repressing Jmjd1a expression in multiple cell lines. Based on these findings, we speculated that JmjC-containing-proteins play non-enzymatic roles by forming functional complex to regulate gene expression. This notion is supported by our two findings that JMJD1A regulates chromatin dynamics by forming complex with SWI/SNF and PPARγ in brown adipocytes and that FBXL10 regulates white adipocyte differentiation by forming a novel polycomb repressive complex including RING1B, BCOR, and SKP1. In addition, our data suggested that JMJD1A regulates the expression of long non-coding RNA.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] JMJD1A is a signal-sensing scaffold that regulates acute chromatin dynamics via SWI/SNF association for thermogenesis2015
Author(s)
Abe Y, Rozqie R, Matsumura Y, Kawamura T, Nakaki R, Tsurutani Y, Tanimura-Inagaki K, Shiono A, Magoori K, Nakamura K, Ogi S, Kajimura S, Kimura H, Tanaka T, Fukami K, Osborne TF, Kodama T, Aburatani H, Inagaki T, Sakai J.
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Journal Title
Nat Commun
Volume: 6
Issue: 1
Pages: 1-14
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] H3K4/H3K9me3 Bivalent Chromatin Domains Targeted by Lineage-Specific DNA Methylation Pauses Adipocyte Differentiation.2015
Author(s)
Matsumura, Y., Nakaki, R., Inagaki, T., Yoshida, A., Kano, Y., Kimura, H., Tanaka, T., Tsutsumi, S., Nakao, M., Doi, T., Fukami, K., Osborne, TF., Kodama, T., Aburatani, H., Sakai, J.
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Journal Title
Molecular Cell
Volume: 60
Issue: 4
Pages: 584-596
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The FBXL10/KDM2B Scaffolding Protein Associates with Novel Polycomb Repressive Complex-1 to Regulate Adipogenesis.2015
Author(s)
Inagaki T, Iwasaki S, Matsumura Y, Kawamura T, Tanaka T, Abe Y, Yamasaki A, Tsurutani Y, Yoshida A, Chikaoka Y, Nakamura K, Magoori K, Nakaki R, Osborne TF, Fukami K, Aburatani H, Kodama T, Sakai J
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Journal Title
J Biol Chem
Volume: 290
Issue: 7
Pages: 4163-77
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] PPARβ/δ activation of CD300a controls intestinal immunity.2014
Author(s)
Tanaka T, Tahara-Hanaoka S, Nabekura T, Ikeda K, Jiang S, Tsutsumi S, Inagaki T, Magoori K, Higurashi T, Takahashi H, Tachibana K, Tsurutani Y, Raza S, Anai M, Minami T, Wada Y, Yokote K, Doi T, Hamakubo T, Auwerx J, Gonzalez FJ, Nakajima A, Aburatani H, Naito M, Shibuya A, Kodama T, Sakai J.
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Journal Title
Sci Rep
Volume: 4
Issue: 1
Pages: 5412-5412
DOI
Related Report
Peer Reviewed / Open Access
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