| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In this study, we planed to prepare individuals of double haploid / haploid using the techniques of gynogenesis in fish, and then we examined the usability of it for the genome sequencing, and construction of the linkage map produced by the SNP typing.
Genome sequencing results show that the N50 contig and scaffold from the double haploid individuals are much longer than those from normal hetero diploid individuals. These results indicate the effectiveness of the use of double-haploid/haploid individuals for de novo sequencing of diploid organisms.
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