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Plant genetic loci controlling rhizosphere microbial community composition

Research Project

Project/Area Number 25640103
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Genome biology
Research InstitutionNational Institute of Genetics

Principal Investigator

IKEO Kazuho  国立遺伝学研究所, 生命情報研究センター, 准教授 (20249949)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsゲノム / 次世代シークエンス / メタゲノム / 16sDNA / 多様性 / バイオインフォマティクス / イネ / 根圏 / 比較ゲノム
Outline of Final Research Achievements

If the root-associated microbial communities are substantially different depending on the plant genotype, it may be possible to breed crop genomes for advantageous rhizosphere composition.  As a first step to addressing these questions we surveyed a series of plant species and cultivars spanning the Oryza genus, growing them in the same nutrient-poor soil and assessing the composition of their rhizosphere and surrounding soil bacteria and archaea using 16s rDNA sequencing. We found that differences between microbes associated with differing plant genotypes were not correlated with phylogenetic distance of the Oryza species or cultivars.
Microbial populations associated with the rhizosphere of wild rice species were less diverse than those associated with cultivars, suggesting that wild species may exert a greater influence on their rhizosphere than cultivated species, and the ability to select rhizosphere microbes may have been eroded during rice domestication.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (2 results)

All 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results)

  • [Journal Article] Efficient screening of long terminal repeat retrotransposons that show high insertion polymorphism via high-throughput sequencing of the primer binding site.2014

    • Author(s)
      Monden Y, Fujii N, Yamaguchi K, Ikeo K, Nakazawa Y, Waki T, Hirashima K, Uchimura Y, Tahara M.
    • Journal Title

      Genome

      Volume: 57(5) Issue: 5 Pages: 245-52

    • DOI

      10.1139/gen-2014-0031

    • NAID

      120005468263

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Human genome network platform: a resource for TFRN analysis.2014

    • Author(s)
      Monma N, Gojobori T, Ikeo K.
    • Journal Title

      Methods Mol Biol

      Volume: 1164 Pages: 147-62

    • DOI

      10.1007/978-1-4939-0805-9_12

    • ISBN
      9781493908042, 9781493908059
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access

URL: 

Published: 2014-07-25   Modified: 2021-04-07  

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