Development of a highly specific genome editing technology for human cells using adeno-associated viral vector
| Project/Area Number |
25640107
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical genome science
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| Research Institution | Aichi Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HOSOKAWA YOSHITAKA 愛知医科大学, 医学部, 教授 (60229193)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | アデノ随伴ウイルスベクター / 遺伝子改変 / CRISPR-Cas9 / ゲノム編集 / 2A |
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| Outline of Final Research Achievements |
In this study, we sought to improve the efficiency of gene targeting mediated by adeno-associated viral (AAV) vectors. We found that the efficiency of gene targeting achieved by AAV-based targeting vectors varies depending on the choice of promoters regulating a selection marker gene within the vectors, and that the use of promoters exhibiting strong transcriptional activity, such as the CMV promoter, in AAV targeting vectors does not result in gene targeting with high efficiency. This study also revealed that a higher gene targeting efficiency is achieved by using a 2A peptide, rather than a commonly used internal ribosomal entry site (IRES), within AAV-based targeting vectors which carry no promoter and exploit the promoters of targeted endogenous genes to regulate a selection marker gene within the vectors (i.e., promoter-trap targeting vectors).
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Plumbagin suppresses tumor cell growth in oral squamous cell carcinoma cell lines2015
Author(s)
Ono T, Ota A, Ito K, Nakaoka T, Karnan S, Konishi H, Furuhashi A, Hayashi T, Yamada Y, Hosokawa Y, Kazaoka Y
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Journal Title
Oral Dis
Volume: -
Issue: 4
Pages: 501-511
DOI
Related Report
Peer Reviewed
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[Journal Article] High-resolution 400K oligonucleotide array comparative genomic hybridization analysis of neurofibromatosis type 1-associated cutaneous neurofibromas2015
Author(s)
Asai A, Karnan S, Ota A, Takahashi M, Damdindorj L, Konishi Y, Hossain E, Konishi H, Nagata A, Yokoo K, Hosokawa Y
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Journal Title
Gene
Volume: 558
Pages: 220-226
Related Report
Peer Reviewed
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[Journal Article] Single copies of mutant KRAS and mutant PIK3CA cooperate in immortalized human epithelial cells to induce tumor formation.2013
Author(s)
Wang GM, Wong HY, Konishi H, Blair BG, Abukhdeir AM, Gustin JP, Rosen DM, Denmeade S, Rasheed Z, Matsui W, Garay JP, Mohseni M, Higgins MJ, Cidado J, Jelovac D, Croessmann S, Cochran R, Karnan S, Konishi Y, Ota A, Hosokawa Y, Argani P, Lauring J, and Park BH.
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Journal Title
Cancer Res
Volume: 73
Issue: 11
Pages: 3248-3261
DOI
Related Report
Peer Reviewed
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