Establishment of a method for safe iPS cell production and acquisition of fully differentiated cells using a human artificial chromosome

• Project/Area Number: 25640108
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical genome science
• Research Institution: Kazusa DNA Research Institute
• Principal Investigator: HASEGAWA Yoshinori 公益財団法人かずさDNA研究所, バイオ研究開発部, 特任研究員 (30387683)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 再生医療 / iPS細胞 / ヒト人工染色体

Outline of Final Research Achievements

First, I generated a tetO-HAC vector carrying Oct4 promoter-driven RFP gene and Nestin promoter-driven GFP gene in HT1080 cells. Next, I introduced the tetO-HAC into MEF cells, and the MEF cells were developed into iPS cells. As a results, I obtained iPS cells carrying the tetO-HAC. I observed RFP signals from the iPS cells.