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Exploration of unculturable bacteria which can be isolated under the co-incubation of protozoa

Research Project

Project/Area Number 25640118
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Conservation of biological resources
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Tarao Mitsunori  東京農工大学, (連合)農学研究科(研究院), 准教授 (60282802)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords微生物保全 / 生物多様性 / 微生物多様性 / 異種間相互作用 / 原生動物 / 細菌群集構造 / 原生生物
Outline of Final Research Achievements

This study hypothesised that some bacteria utilise protozoan extracellular exudates (PECEs), as signal factors or growth promotion factors. By using PECEs derived from ciliate Tetrahymena thermophia, as a model protozoa, two outcomes were obtained. (1) Bacterial communities of freshwater were incubated in the liquid medium with PECEs, and the community structure was analysed sequentially. As a result, it is suggested that PECEs contribute to stabilise bacterial community structure. (2) Bacterial communities of freshwater were incubated on the agar plate with PECEs. As a result, some bacterial strains which formed colonies only on the agar plate with PECEs were isolated. The isolates did not form colonies on the agar plate without PECEs. These results suggest that some bacterial strains in natural environments are stimulated their growth or metabolic activities by PECEs.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2014

All Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] 廃水処理において亜酸化窒素の生産と窒素除去を両立させるC/N比の検討2016

    • Author(s)
      森脇芙美・多羅尾光徳
    • Organizer
      日本環境学会第42回研究発表会
    • Place of Presentation
      東京都市大学(神奈川県横浜市)
    • Year and Date
      2016-06-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] N2O emission by denitrifying bacteria isolated from freshwater environments by using a medium with low nitrate2015

    • Author(s)
      Mitsunori Tarao, Maki Shinohara, Hiraohiko Nagano, Johji Kudoh, Yuta Mizuuchi
    • Organizer
      The 7th Japan-Taiwan-Korea International Symposium on Microbial Ecology
    • Place of Presentation
      茨城県土浦市
    • Year and Date
      2015-10-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細菌群集構造に原生動物の細胞外分泌物が及ぼす影響2015

    • Author(s)
      濱部惇・多羅尾光徳
    • Organizer
      第49回日本水環境学会年会
    • Place of Presentation
      金沢大学,金沢市,石川県
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 細菌群集構造に原生動物の捕食と細胞外分泌物が及ぼす影響2014

    • Author(s)
      濱部惇・多羅尾光徳
    • Organizer
      環境微生物系学会合同大会
    • Place of Presentation
      浜松アクトシティコングレスセンター,浜松市,静岡県
    • Year and Date
      2014-10-21 – 2014-10-24
    • Related Report
      2014 Research-status Report
  • [Presentation] Comparative studies of colony formation of freshwater bacteria on agar plate with or without protozoan culture solution2014

    • Author(s)
      Motoko Sasaki, Daisuke Komatsu, Haruna Kobayashi, Mitsunori Tarao
    • Organizer
      15th International Symposium on Microbial Ecology
    • Place of Presentation
      COEX Convention Center, Seoul, Republic of Korea
    • Year and Date
      2014-08-24 – 2014-08-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Effect of composition of microbial community on susceptibility of a chlorophenol-degrading bacterium to copper ion2014

    • Author(s)
      Mitsunori Tarao, Keiichiro Okayama
    • Organizer
      15th International Symposium on Microbial Ecology
    • Place of Presentation
      COEX Convention Center, Seoul, Republic of Korea
    • Year and Date
      2014-08-24 – 2014-08-29
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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