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Basic reserch of long term preservation at room temperature for mammalian somatic cells by freeze drying

Research Project

Project/Area Number 25640121
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Conservation of biological resources
Research InstitutionKochi University

Principal Investigator

MATSUKAWA Kazutsugu  高知大学, 教育研究部総合科学系, 准教授 (00532160)

Project Period (FY) 2013-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords凍結乾燥 / 哺乳類体細胞 / 遺伝資源保存 / 哺乳動物体細胞
Research Abstract

This research carried out basic study as preservation technique of a new mammalian somatic cells by freeze-drying. In bovine fibroblast cells, total aomount of protein and the number of gamma-tubulin decreased after freeze-drying. Furthermore, mitochondrial membrane potential was not observed in the cell after freeze-drying. It was thought that addition of trehalose and epigallocatechin gallate (EGCG)in a cell culture medium was ineffective to protection of a cell membrane and organelle. However, when temperature higher than a glass transition point after FD, trehalose and EGCG could prevent damage in nuclear DNA. These result suggested that freeze-dried somatic cells has the possibility of preservation at room temperature by trehalose and EGCG .

Report

(2 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (4 results)

All 2014 2013

All Presentation (4 results)

  • [Presentation] アスコルビン酸2リン酸の体外成熟培地への添加はウシ体外受精胚の発生能を向上させる2014

    • Author(s)
      細川真美、段家春香、竹中由布、枝重圭祐、葛西孫三郎、松川和嗣
    • Organizer
      第118回日本畜産学会大会
    • Place of Presentation
      つくば市
    • Related Report
      2013 Final Research Report
  • [Presentation] スコルビン酸2リン酸の体外成熟培地への添加はウシ体外受精胚の発生能を向上させる2014

    • Author(s)
      細川 真美,段家 春花,竹中 由布,枝重 圭祐,葛西 孫三郎,松川 和嗣
    • Organizer
      第118回日本畜産学会大会
    • Place of Presentation
      つくば市つくば国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 凍結乾燥後のウシ線維芽細胞はゲノムDNAを維持している2013

    • Author(s)
      郡七海,松川和嗣
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋市
    • Related Report
      2013 Final Research Report
  • [Presentation] 凍結乾燥後のウシ線維芽細胞はゲノムDNAを維持している2013

    • Author(s)
      郡 七海,松川 和嗣
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋市ウインク愛知
    • Related Report
      2013 Annual Research Report

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Published: 2014-07-25   Modified: 2016-11-25  

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