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Analysis of DNA demethylation by Glial cells missing genes

Research Project

Project/Area Number 25650005
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Molecular biology
Research InstitutionShiga University of Medical Science

Principal Investigator

Hitoshi Seiji  滋賀医科大学, 医学部, 教授 (70300895)

Co-Investigator(Renkei-kenkyūsha) TOMITA KOICHI  高知大学, 医学部, 准教授 (80314285)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsエピジェネティクス / 能動的DNA脱メチル化 / 受精卵 / 能動的脱メチル化 / リプログラミング
Outline of Final Research Achievements

Epigenetic mechanisms include DNA methylation and histone modifications, which occur without changing the DNA sequence and alter gene expression. The DNA methylation was frequently found in transcriptionally inactive gene loci and it was thought to be a unidirectional and persistent process to silence gene action. However, recent findings have challenged this notion and suggest that 5-methylcytosine can be reversed to cytosine even in postmitotic neurons by active DNA demethylation process. We have recently identified Gcm genes are responsible to the active demethylation in early mammalian development. Here, we investigated biochemical characteristics of the active DNA demethylation by Gcm and found that it could work in a quite novel fashion. In addition, we observed that Gcm2 deficient embryos made by in vitro fertilization showed abnormal cell divisions by 4-cell stage. These results suggest that Gcm genes play critical roles in the epigenetic regulation of early embryos.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (22 results)

All 2016 2015 2014 2013 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 2 results,  Invited: 6 results) Remarks (1 results)

  • [Journal Article] The dorsoventral boundary of the germinal zone is a specialized niche for the generation of cortical oligodendrocytes during a restricted temporal window.2016

    • Author(s)
      Naruse M, Ishino Y, Kumar A, Ono K, Takebayashi H, Yamaguchi M, Ishizaki Y, Ikenaka K, Hitoshi S
    • Journal Title

      Cerebral Cortex

      Volume: 26

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neural stem cells and neuro/gliogeneis in the central nervous system: understanding the structural and functional plasticity of the developing, mature, and diseased brain.2016

    • Author(s)
      Yamaguchi M, Seki T, Imayoshi I, Tamamaki N, Hayashi Y, Tatebayashi Y, Hitoshi S
    • Journal Title

      Journal of Physiological Sciences

      Volume: 66

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heterozygous Polg mutation causes motor dysfunction due to mtDNA deletions.2014

    • Author(s)
      Fuke S, Kametani M, Yamada K, Kasahara T, Kubota-Sakashita M, Kujoth GC, Prolla TA, Hitoshi S, Kato T
    • Journal Title

      Annals of Clinical and Translational Neurology

      Volume: 1 Issue: 11 Pages: 909-920

    • DOI

      10.1002/acn3.133

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Determination of major sialylated N-glycans and identification of branched sialylated N-glycans that dynamically change their content during development in the mouse cerebral cortex.2014

    • Author(s)
      Torii T, Yoshimura T, Narumi M, Hitoshi S, Takaki Y, Tsuji S, Ikenaka K
    • Journal Title

      Glycoconjugate Journal

      Volume: 31(9) Pages: 671-683

    • DOI

      10.1007/s10719-014-9566-2

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mechanisms for interferon-α-induced depression and neural stem cell dysfunction.2014

    • Author(s)
      Zheng LS, Hitoshi S, Kaneko N, Takao K, Miyakawa T, Tanaka Y, Xia H, Kalinke U, Kudo K, Kanba S, Ikenaka K, Sawamoto K.
    • Journal Title

      Stem Cell Reports

      Volume: 3(1) Issue: 1 Pages: 73-84

    • DOI

      10.1016/j.stemcr.2014.05.015

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Bre1a, a histone H2B ubiquitin ligase, regulates the cell cycle and differentiation of neural precursor cells.2014

    • Author(s)
      Ishino Y, Hayashi Y, Naruse M, Tomita K, Sanbo M, Fuchigami T, Fujiki R, Hirose K, Toyooka Y, Fujimori T, Ikenaka K, Hitoshi S.
    • Journal Title

      The Journal of Neuroscience

      Volume: 34 Issue: 8 Pages: 3067-78

    • DOI

      10.1523/jneurosci.3832-13.2014

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Lewis X-related α1,3-Fucosyltransferase, Fut10, Is Required for the Maintenance of Stem Cell Populations.2013

    • Author(s)
      Kumar A, Torii T, Ishino Y, Muraoka D, Yoshimura T, Togayachi A, Narimatsu H, Ikenaka K, Hitoshi S.
    • Journal Title

      J Biol Chem.

      Volume: 288 Issue: 40 Pages: 28859-28868

    • DOI

      10.1074/jbc.m113.469403

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] The Lewis X-related α1,3-fucosyltransferase, Fut10, is required for the maintenance of stem cell populations.2015

    • Author(s)
      Hitoshi S
    • Organizer
      BMB2015
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] The dorsoventral boundary of the germinal zone is a specialized niche for the generation of cortical oligodendrocytes.2015

    • Author(s)
      Hitoshi S, Asuke J, Naruse M, Ikenaka K
    • Organizer
      第58回日本神経化学会
    • Place of Presentation
      大宮
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] Epigenetics in the maintenance of neural stem cells.2015

    • Author(s)
      Hitoshi S
    • Organizer
      25th Meeting of the International Society for Neurochemistry
    • Place of Presentation
      Cairns
    • Year and Date
      2015-08-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The oligodendroglial abnormalities in mood disorders: a study of human and monkey post mortem brain.2015

    • Author(s)
      Hayashi Y, Fuke S, Fuchigami T, Koyama N, Tatebayashi Y, Hitoshi S
    • Organizer
      25th Meeting of the International Society for Neurochemistry
    • Place of Presentation
      Cairns
    • Year and Date
      2015-08-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 神経幹細胞からオリゴデンドロサイト系譜細胞が産生される分子機序2015

    • Author(s)
      等 誠司, 成瀬雅衣, 池中一裕
    • Organizer
      第120回日本解剖学会・第92回日本生理学会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] The Lewis X-related α1,3-fucosyltransferase, Fut10, is required for the maintenance of stem cell populations.2014

    • Author(s)
      Hitoshi S, Fuke S, Kumar A, Ikenaka K
    • Organizer
      Society for Glycobiology & Japanese Society of Carbohydrate Research 2014 Joint Annual Meeting
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2014-11-16 – 2014-11-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Relationship between cortical neurogenesis and mood; a study of primate.2014

    • Author(s)
      Hayashi Y, Fuke S, Fuchigami T, Koyama N, Tatebayashi Y, Hitoshi S
    • Organizer
      第57回日本神経化学会, 第36回日本生物学的精神医学会 合同年会
    • Place of Presentation
      奈良
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Analysis of the mono-ubiquitylation of histone H2B associated with differentiation into neural stem cell linage.2014

    • Author(s)
      Fuke S, Ishino Y, Hayashi Y, Nakabayashi K, Ikenaka K, Hitoshi S
    • Organizer
      第57回日本神経化学会, 第36回日本生物学的精神医学会 合同年会
    • Place of Presentation
      奈良
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Research-status Report
  • [Presentation] The effects of maternal separation on the anxiety- and fear-related behavior in the mice.2014

    • Author(s)
      Koyama N, Jia X, Hitoshi S
    • Organizer
      第37回日本神経科学学会 (Neuroscience 2014)
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Epigenetic mechanisms underlying the generation and differentiation of neural stem cells.2014

    • Author(s)
      Hitoshi S, Hayashi Y, Fuchigami T, Fuke S, Ishino Y, Ikenaka K
    • Organizer
      12th Annual Meeting of the International Society for Stem Cell Research
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2014-06-18 – 2014-06-21
    • Related Report
      2014 Research-status Report
  • [Presentation] 恐怖回避モデルの動物モデル探索のための予備的研究2014

    • Author(s)
      小山なつ, 賈 暁静, 福家 聡, 等 誠司
    • Organizer
      第36回日本疼痛学会
    • Place of Presentation
      大阪
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Role of Kruppel-like factor 5 in the proliferation and radial migration of neural precursor cells.2013

    • Author(s)
      Fuchigami T, Hayashi Y, Ema M, Kuroda A, Hitoshi S
    • Organizer
      43rd Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Epigenetics underlying the maintenance of neural stem cells.2013

    • Author(s)
      Hitoshi S
    • Organizer
      Neuro2013
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Epigenetic mechanisms underlying the generation and maintenance of mammalian neural stem cells.2013

    • Author(s)
      Hitoshi S
    • Organizer
      第7回エピジェネティクス研究会年会
    • Place of Presentation
      奈良
    • Related Report
      2013 Research-status Report
    • Invited
  • [Remarks] 統合臓器生理学部門 研究内容

    • URL

      http://www.shiga-med.ac.jp/~hqphysi1/physiol1/research/index-re.html

    • Related Report
      2015 Annual Research Report 2014 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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