DNA methylation coupled with transcription elongation machinery
Project/Area Number |
25650007
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Molecular biology
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Research Institution | Osaka University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
Shoji Tajima 大阪大学, たんぱく質研究所, 教授 (50132931)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | DNAメチル化 / 転写伸長 / DNAのメチル化機構 / 転写伸長因子 |
Outline of Final Research Achievements |
Recent whole genome bisulfite sequencing analysis with next generation sequencer reveals that DNA methylation exist not only at promoter, but also at gene bodies of highly transcribed genes. Previously, I purified the Dnmt3a complex from mouse embryonic stem cell (mESC), and found that Rpb1 and Supt6h, which are components of transcriptional elongation machinery, were co-purified with Dnmt3a. To elucidate the mechanism underling in the gene body methylation, I focused in an interaction of Dnmt3a and transcriptional elongation machinery. I found that (1) Dnmt3a and Rpb1 bound in mESC, NIH3T3 and N1E115, (2) Dnmt3a localized on gene body of Atp5b gene, which is highly transcribed in mESC, in the same manner with the elongating Rpb1 (phosphorylated CTD-Ser2) and H3K36me3, (3) both of Dnmt3a and Dnmt3b contributed on the gene body methylation.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Structural basis of the versatile DNA recognition ability of the methyl-CpG binding domain of methyl-CpG binding domain protein 4.2013
Author(s)
Otani, J., Arita, K., Kato, T., Kinoshita, M., Kimura, H., Suetake, I., Tajima, S., Ariyoshi, M., Shirakawa, M.
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Journal Title
J Biol. Chem.
Volume: 288
Issue: 9
Pages: 6351-6362
DOI
Related Report
Peer Reviewed
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[Journal Article] Genome engineering of mammalian haploid embryonic stem cells using the Cas9/RNA system2013
Author(s)
Horii T, Morita S, Kimura M, Kobayashi R, Tamura D, Takahashi RU, Kimura H, Suetake I, Ohata H, Okamoto K, Tajima S, Ochiya T, Abe Y, Hatada I
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Journal Title
PeerJ
Volume: 1
Pages: e230-e230
DOI
Related Report
Peer Reviewed
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[Presentation] Relative positions of 5-hydroxymethylcytosine and 5-methylcytosine at promoter correlate with the level of gene expression.2013
Author(s)
Kimura, H., Suetake, I., Tooi, N., Kawakami, T., Thomoto, T., Aiba, K., Nakatsuji, N and Shoji Tajima
Organizer
International Symposium on Transcription and Metabolism
Place of Presentation
兵庫県淡路夢舞台
Related Report