| Project/Area Number |
25650009
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Tokyo Institute of Technology (2014-2015)
Osaka University (2013) |
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Principal Investigator |
Sato Yuko 東京工業大学, 生命理工学研究科, 科学研究費研究員 (70435882)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | エピジェネティクス / イメージング / クロマチン / ライブイメージング / 蛍光プローブ / ヒストン修飾 / 細胞内抗体 / scFv / 細胞核 / 遺伝子 |
|---|
| Outline of Final Research Achievements |
This project aimed to visualize the dynamics of histone modification in the specific gene loci in living cells. To visualize specific genome loci, we searched repetitive DNA sequence enriched in single loci and designed TALE protein. However, TALEs that are expected to bind to these sequences failed to label them in living cells. On the other hand, using a mintbody specific to phosphorylated RNA polymerase II combined with ANCHOR system that labeled the specific locus, active transcription at the single gene locus was visualized in human cells.
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