| Project/Area Number |
25650010
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University |
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Principal Investigator |
MASUKATA Hisao 大阪大学, 理学(系)研究科(研究院), 教授 (00199689)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | PP1 / PP2A / Rif1 / テロメア / 複製開始点 / 染色体 / 分裂酵母 / 複製開始制御 / DNA複製 / CDK / DDK / フォスファターゼ |
|---|
| Outline of Final Research Achievements |
Chromosomal DNA replication initiates at many loci called replication origins on the chromosome, and individual replication origins are activated at specific timing in S phase. Since the process of initiation requires phosphorylations of initiation factors by protein kinase, such as cyclin-dependent kinase (CDK) and Dbf4-dependent kinase (DDK), we asked whether protein phosphatase, such as PP1 and PP2A, are involved in the control. Using the auxin-induced protein degradation system (AID) in fission yeast, we found that PP1 is essential for the control of replication timing, whereas PP2A is not required.
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