• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of factors involved in the eukaryotic mismatch repair reaction

Research Project

Project/Area Number 25650011
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Molecular biology
Research InstitutionOsaka University

Principal Investigator

TAKAHASHI Tatsuro  大阪大学, 理学(系)研究科(研究院), 助教 (50452420)

Research Collaborator KAWASOE Yoshitaka  
TERUI Riki  
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsミスマッチ修復 / クロマチン / DNA損傷応答 / DNA複製 / ツメガエル / 卵抽出液 / 試験管内系 / DNA修復 / 染色体 / 無細胞系
Outline of Final Research Achievements

Eukaryotic chromosomal DNA is wrapped around histones to be assembled into chromatin, whose dynamic remodeling is linked to various DNA transactions. The mismatch repair system maintains DNA replication fidelity by correcting replication errors. Impairment of this system thus leads to oncogenesis in human. Here, we sought factors involved in the mismatch repair reaction in the context of chromatin, using Xenopus egg extract that promotes chromatin assembly in vitro. We have identified at least 9 factors whose involvements in mismatch repair have not previously been documented, and 3 of which were chromatin-related factors. Having identified these new factors, this work established an important step toward understanding of the mismatch repair reaction on chromatin.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (22 results)

All 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (19 results) (of which Invited: 6 results)

  • [Journal Article] RecQ4 promotes the conversion of the pre-initiation complex at a site-specific origin for DNA unwinding in Xenopus egg extracts2015

    • Author(s)
      Sanuki, Kubota, Kanemaki MT, Takahashi TS, Mimura S, Takisawa H
    • Journal Title

      Cell Cycle

      Volume: 14-7 Issue: 7 Pages: 1010-23

    • DOI

      10.1080/15384101.2015.1007003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thymine DNA glycosylase is a CRL4Cdt2 substrate.2014

    • Author(s)
      Slenn TJ, Morris B, Havens CG, Freeman RM Jr, Takahashi TS, Walter JC.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 289 Issue: 33 Pages: 23043-55

    • DOI

      10.1074/jbc.m114.574194

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MutS stimulates the endonuclease activity of MutL in an ATP-hydrolysis-dependent manner.2013

    • Author(s)
      Shimada, A., Kawasoe, Y., Hata, Y., Takahashi, T.S., Masui, R., Kuramitsu, S., Fukui, K.
    • Journal Title

      FEBS Journal

      Volume: 280 (14) Issue: 14 Pages: 3467-3479

    • DOI

      10.1111/febs.12344

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] PCNAとXEco2はDNA複製と協調したSororin染色体結合反応に機能する2014

    • Author(s)
      高橋 達郎
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市 パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ミスマッチ修復因子MutSαはPCNAのDNAからの解離を阻害することで新生鎖の記憶を保持する2014

    • Author(s)
      河添 好孝
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市 パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] MutSα依存的なミスマッチ塩基対周辺のヌクレオソーム排除反応2014

    • Author(s)
      照井 利輝
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市 パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] MutSα-dependent exclusion of nucleosomes from mismatch sites in Xenopus egg extracts2014

    • Author(s)
      高橋 達郎
    • Organizer
      The 9th 3R Symposium
    • Place of Presentation
      静岡県御殿場市 ホテル時之栖
    • Year and Date
      2014-11-17 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] PCNA and XEco2 couple chromatin loading of Sororin to DNA replication in Xenopus egg extracts2014

    • Author(s)
      東 寅彦
    • Organizer
      The 9th 3R Symposium
    • Place of Presentation
      静岡県御殿場市 ホテル時之栖
    • Year and Date
      2014-11-17 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] MutSα prevents dissociation of PCNA from DNA to keep strand information for eukaryotic mismatch repair2014

    • Author(s)
      河添 好孝
    • Organizer
      The 9th 3R Symposium
    • Place of Presentation
      静岡県御殿場市 ホテル時之栖
    • Year and Date
      2014-11-17 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] MutSα prevents PCNA unloading to keep strand information for eukaryotic mismatch repair2014

    • Author(s)
      高橋 達郎
    • Organizer
      国立遺伝学研究所・研究集会「染色体 DNA の安定維持の分子メカニズム」
    • Place of Presentation
      静岡県三島市 国立遺伝学研究所
    • Year and Date
      2014-11-06 – 2014-11-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] MutSα prevents dissociation of PCNA from DNA to keep strand information for eukaryotic mismatch repair2014

    • Author(s)
      高橋 達郎
    • Organizer
      The 5th US-Japan DNA repair meeting
    • Place of Presentation
      徳島県鳴門市 グランドエクシブ鳴門
    • Year and Date
      2014-10-28 – 2014-10-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] ミスマッチ修復とクロマチン複製のカップリング2014

    • Author(s)
      高橋 達郎
    • Organizer
      蛋白研セミナー 『染色体伝承の分子背景:複製から染色体分離まで』
    • Place of Presentation
      大阪府吹田市 大阪大学蛋白質研究所 1 階講堂
    • Year and Date
      2014-09-25 – 2014-09-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] MutSα-dependent eviction of nucleosomes around mismatch sites in Xenopus egg extracts2014

    • Author(s)
      Tatsuro Takahashi, Riki Terui, Kanae Taki, koji Nagao, Takuro Nakagawa, Yumiko Kubota, Haruhiko Takisawa, Chikashi Obuse, Hisao Masukata
    • Organizer
      International Conference: Replication, repair, and transcription; coupling mechanisms and chromatin dynamics for genome integrity
    • Place of Presentation
      京都市左京区 京都大学 時計台100周年記念ホール
    • Related Report
      2013 Research-status Report
  • [Presentation] 真核生物ミスマッチ修復機構とクロマチン動態、DNA損傷応答機構のクロストーク2014

    • Author(s)
      高橋達郎
    • Organizer
      平成25年度「がん研究分野の特性等を踏まえた支援活動」 公開シンポジウム
    • Place of Presentation
      東京都千代田区 学術総合センター
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Essential roles of the N-terminal region of XEco2 acetyltransferase for cohesion establishment in Xenopus egg extracts2013

    • Author(s)
      Torahiko Higashi, Takuro Nakagawa, Hisao Masukata, Tatsuro Takahashi
    • Organizer
      Cold Spring Harbor Laboratory Meeting "Eukaryotic DNA replication & Genome Maintenance"
    • Place of Presentation
      Cold Spring Harbor, NY
    • Related Report
      2013 Research-status Report
  • [Presentation] MutSα-dependent eviction of nucleosomes around mismatch sites in Xenopus egg extracts2013

    • Author(s)
      Riki Terui, Kanae Taki, Torahiko Higashi, Takuro Nakagawa, Hisao Masukata, Tatsuro Takahashi
    • Organizer
      Cold Spring Harbor Laboratory Meeting "Eukaryotic DNA replication & Genome Maintenance"
    • Place of Presentation
      Cold Spring Harbor, NY
    • Related Report
      2013 Research-status Report
  • [Presentation] Evidence that DNA-loaded PCNA acts as the strand marker for eukaryotic mismatch repair2013

    • Author(s)
      Yoshitaka Kawasoe, Toshiki Tsurimoto,Takuro Nakagawa, Hisao Masukata, Tatsuro Takahashi
    • Organizer
      Cold Spring Harbor Laboratory Meeting "Eukaryotic DNA replication & Genome Maintenance"
    • Place of Presentation
      Cold Spring Harbor, NY
    • Related Report
      2013 Research-status Report
  • [Presentation] MutSα-dependent eviction of nucleosomes around mismatch sites in Xenopus egg extracts2013

    • Author(s)
      Tatsuro Takahashi, Riki Terui, Kanae Taki, Koji Nagao, Takuro Nakagawa, Uimiko Kubota, Haruhiko Takisawa, Chikashi Obuse, Hisao Masukata
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      兵庫県神戸市 神戸国際会議場
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] ミスマッチ周辺でのMutS依存的ヌクレオソーム除去反応に関わる因子の探索2013

    • Author(s)
      Riki Terui, Kanae Taki, koji Nagao, Takuro Nakagawa, Yumiko Kubota, Haruhiko Takisawa, Chikashi Obuse, Hisao Masukata
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      兵庫県神戸市 神戸国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] ミスマッチ修復機構がクロマチン複製の場で機能するための機構2013

    • Author(s)
      高橋達郎
    • Organizer
      国立遺伝学研究所・研究会「染色体 DNA の安定維持の分子メカニズム」
    • Place of Presentation
      静岡県三島市 国立遺伝学研究所
    • Related Report
      2013 Research-status Report
  • [Presentation] ミスマッチ修復機構がクロマチン複製の場で機能するための機構2013

    • Author(s)
      高橋達郎
    • Organizer
      国立遺伝学研究所・研究会「クロマチンによる遺伝情報のエピジェネティック制御機構」
    • Place of Presentation
      静岡県三島市 国立遺伝学研究所
    • Related Report
      2013 Research-status Report
  • [Presentation] Coupling of cohesin acetylation to the process of DNA replication in Xenopus egg extracts

    • Author(s)
      Torahiko Higashi, Takuro Nakagawa, Hisao Masukata, Tatsuro Takahashi
    • Organizer
      The 18th IMCB Symposium
    • Place of Presentation
      東京都文京区 東京大学弥生講堂
    • Related Report
      2013 Research-status Report
    • Invited

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi