Novel genome-organization mechanism by telomere-binding proteins

• Project/Area Number: 25650012
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: Kanoh Junko 大阪大学, たんぱく質研究所, 准教授 (10323809)
• Research Collaborator: HASEGAWA Yudai
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: テロメア / DNA複製 / ゲノム / 複製開始点 / クロマチン / 染色体 / 核 / 核内配置

Outline of Final Research Achievements

Replication of chromosomal DNA starts at the replication origins at fixed timing during S phase. In this study, we investigated the effect of telomere defects in genome-wide replication timing. We observed the accelerated replication at some late origins when the telomere DNA was abnormally elongated by the Rap1 or Poz1 deletions. Furthermore, we found that the localization of PP1 phosphatase was decreased at the late origins in those mutants. Our results indicated that the telomere length maintenance is important for the normal replication timing of chromosome DNA.

Research Products

• [Int'l Joint Research] 上海国立蛋白質研究所(中国)
• [Journal Article] Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing. (2016)
  • Author(s): Tashiro S, Handa T, Matsuda A, Ban T, Takigawa T, Miyasato K, Ishii K, Kugou K, Ohta K, Hiraoka Y, Masukata H, Kanoh J.
  • Journal Title: Nature Commun. Volume: 7 Issue: 1 Pages: 10393-10393
  • DOI: 10.1038/ncomms10393
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fission yeast telomere-binding protein Taz1 is a functional but not a structural counterpart of human TRF1 and TRF2. (2015)
  • Author(s): Deng, W., Wu, J., Wang, F., Kanoh, J., Dehe, PM., Inoue, H., Chen, J., and Lei, M.
  • Journal Title: Cell Research Volume: 25 Issue: 7 Pages: 881-884
  • DOI: 10.1038/cr.2015.76
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 第８章ゲノムを支えるインターメアの機能（２）ーテロメア機能とサブテロメアー (2015)
  • Author(s): 加納純子
  • Journal Title: 「ゲノムを司るインターメア」Dojin Biosciene Volume: 23 Pages: 87-100
• [Journal Article] Receptor for activated C-kinase (RACK1) homolog Cpc2 facilitates the general amino acid control response through Gcn2 kinase in fission yeast (2013)
  • Author(s): Tarumoto, Y. Kanoh, J. Ishikawa, F.
  • Journal Title: The Journal of Biological Chemistry Volume: 288(26) Issue: 26 Pages: 19260-19268
  • DOI: 10.1074/jbc.m112.445270
  • Peer Reviewed / Open Access
• [Journal Article] Release of chromosomes from the nuclear envelope: a universal mechanism for eukaryotic mitosis? (2013)
  • Author(s): Kanoh J.
  • Journal Title: Nucleus Volume: 4 Issue: 2 Pages: 100-104
  • DOI: 10.4161/nucl.23984
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transcription-induced chromatin association of RNA surveillance factors mediates facultative heterochromatin formation in fission yeast (2013)
  • Author(s): Tashiro, S.
  • Journal Title: Genes to Cells Volume: 18 Issue: 4 Pages: 327-339
  • DOI: 10.1111/gtc.12038
  • Peer Reviewed / Open Access
• [Journal Article] 第５章「テロメア」 (2013)
  • Author(s): 加納純子
  • Journal Title: Dojin Biosciene 11「染色体と細胞核のダイナミクス」 Volume: 11 Pages: 63-77
• [Presentation] サブテロメア相同配列全破壊によって見えてくる染色体機能 (2016)
  • Author(s): 田代三喜、西原祐輝、久郷和人、太田邦史、加納純子
  • Organizer: 第３３回染色体ワークショップ
  • Place of Presentation: 松島一の坊、宮城
  • Year and Date: 2016-01-12
• [Presentation] サブテロメアの機能を探る (2015)
  • Author(s): 加納純子
  • Organizer: クロマチンデコーディング2015年度第１回研究会
  • Place of Presentation: 国際高等研究所、奈良
  • Year and Date: 2015-12-19
  • Invited
• [Presentation] サブテロメアの新規機能の解明 (2015)
  • Author(s): Tashiro, S., Handa, T., Matsuda, A., Ban, T., Takigawa, T., Miyasato, K., Ishii, K., Kugou, K., Ohta, K., Hiraoka, Y., Masukata, H., and Kanoh, J.
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド、神戸
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] 分裂酵母サブテロメア相同DNA領域完全欠失株の作製と解析 (2015)
  • Author(s): 田代三喜、西原祐輝、久郷和人、太田邦史、加納純子
  • Organizer: 酵母遺伝学フォーラム第４８回研究報告会
  • Place of Presentation: 広島大学、広島
  • Year and Date: 2015-08-31
• [Presentation] Dissection of subtelomere functions (2015)
  • Author(s): Junko Kanoh
  • Organizer: Forefront of chromosome biology
  • Place of Presentation: 京都大学、京都
  • Year and Date: 2015-08-10
  • Int'l Joint Research / Invited
• [Presentation] Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing. (2015)
  • Author(s): Tashiro, S., Handa, T., Matsuda, A., Ban, T., Takigawa, T., Miyasato, K., Ishii, K., Kugou, K., Ohta, K., Hiraoka, Y., Masukata, H., and Kanoh, J.
  • Organizer: 第８回国際分裂酵母会議
  • Place of Presentation: 生田神社、神戸
  • Year and Date: 2015-06-21
  • Int'l Joint Research / Invited
• [Presentation] Spindle assembly checkpoint protein Sgo2 regulates silenced chromatin formation and DNA replication timing at subtelomeres (2015)
  • Author(s): Tashiro, S., Handa, T., Matsuda, A., Ban, T., Takigawa, T., Miyasato, K., Ishii, K., Kugou, K., Ohta, K., Hiraoka, Y., Masukata, H., and Kanoh, J.
  • Organizer: Telomeres and Telomerase, Cold Spring Harbor Laboratory meeting
  • Place of Presentation: Cold Spring Harbor Laboratory, NY, USA
  • Year and Date: 2015-04-28
  • Int'l Joint Research
• [Presentation] Roles of protein complexes at chromosome end (2015)
  • Author(s): Junko Kanoh
  • Organizer: 1st trilateral workshop for frontier protein studies
  • Place of Presentation: 北京大学、中国
  • Year and Date: 2015-04-23
  • Int'l Joint Research / Invited
• [Presentation] テロメア結合タンパク質Rap1のCK2によるリン酸化はテロメアの核内配置とヘテロクロマチン構造を制御する (2014)
  • Author(s): 加納純子
  • Organizer: 第３２回染色体ワークショップ
  • Place of Presentation: 安芸グランドホテル、広島
  • Year and Date: 2014-12-15 – 2014-12-17
• [Presentation] Novel functions of shugoshin 2 (Sgo2) at telomere-adjacent region in interaphase (2014)
  • Author(s): 加納純子
  • Organizer: 3R symposium
  • Place of Presentation: 御殿場高原ホテル、静岡
  • Year and Date: 2014-11-17 – 2014-11-21
  • Invited
• [Presentation] サブテロメア領域の新規機能の解明 (2014)
  • Author(s): 加納純子
  • Organizer: 日本遺伝学会第８６回大会
  • Place of Presentation: 長浜バイオ大学、滋賀
  • Year and Date: 2014-09-17 – 2014-09-19
  • Invited
• [Presentation] 染色体末端の神秘 (2014)
  • Author(s): 加納純子
  • Organizer: 第２１回酵母合同シンポジウム
  • Place of Presentation: 東京大学、東京
  • Year and Date: 2014-09-03 – 2014-09-04
  • Invited
• [Presentation] Fission yeast without subtelomeres (2014)
  • Author(s): 加納純子
  • Organizer: EMBO conference, Telomeres, telomerase and disease
  • Place of Presentation: ブリュッセル、ベルギー
  • Year and Date: 2014-04-30 – 2014-05-04
• [Presentation] Rif3, a novel Rap1-interacting factor in S. pombe. (2013)
  • Author(s): Kanoh, J., and Tanakam, M.
  • Organizer: Telomeres & Telomerase
  • Place of Presentation: Cold Spring Harbor Laboratory (米国ニューヨーク州）
• [Presentation] Structure and functions of subtelomere. (2013)
  • Author(s): Kanoh, J.
  • Organizer: Message from yeast to epgenetics
  • Place of Presentation: Grandia Housen（福井市）
  • Invited
• [Presentation] 分裂酵母のサブテロメア領域の機能解析 (2013)
  • Author(s): 田代三喜, 坂琢人, 西原祐輝, 宮里和実, 平岡泰, 石井浩二郎, 加納純子
  • Organizer: 第４６回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 東北学院大学（仙台市）
• [Presentation] テロメア隣接領域の機能 (2013)
  • Author(s): 加納純子
  • Organizer: 国立遺伝学研究所研究会「染色体DNAの安定維持の分子メカニズム」
  • Place of Presentation: 国立遺伝学研究所（三島市）
  • Invited
• [Presentation] テロメア隣接領域の機能 (2013)
  • Author(s): 加納純子
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（神戸市）
  • Invited
• [Book] 生物学辞典第５版 (2013)
  • Author(s): 加納純子（他）
  • Total Pages: 2171
  • Publisher: 岩波書店