| Project/Area Number |
25650016
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
ISHIMORI Koichiro 北海道大学, 理学(系)研究科(研究院), 教授 (20192487)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIDA Takeshi 北海道大学, 大学院理学研究院, 准教授 (30343742)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | シトクロム酸化酵素 / ナノディスク / 呼吸鎖電子伝達系 / NMR / MSP / 安定同位体 |
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| Outline of Final Research Achievements |
To discuss the electron transfer mechanism in the respiratory chain in mitochondria, the reconstitution of an isotope labelled key enzyme, cytochrome c oxidase, into the nano-disc, a mimic of membrane in cells, has been examined, which allows us to measure the high resolution NMR spectra of the membrane-bound proteins under the physiological condition. The expression, purification and reconstitution of the bacterial cytochrome c oxidase into the nano-disc were confirmed, leading to the high resolution structural analysis of high molecular weight membrane bound proteins.
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