Analysis of Rab membrane insertion mechanism using artificial membranes

• Project/Area Number: 25650018
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: ITO Sakurako 東京大学, 分子細胞生物学研究所, 助教 (60597152)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: Rab / 膜 / GEF / 小胞 / 膜たんぱく質 / 膜タンパク質

Outline of Final Research Achievements

In eukaryotic cells, Rab family small GTPases present the identity of each membrane fractions. Rab forms a complex with RabGDI in cytosol, and when necessary, appropriate Rab needs to be detached from RabGDI and inserted into the membrane. In this study, we have tried to reconstruct the Rab insertion system on the artificial membrane. Yip family transmembrane proteins and RabGEFs are proposed to function in the Rab insertion system. We have successfully prepared these components, which is necessary for the reconstruction of the Rab insertion system.

Research Products

• [Journal Article] Mechanisms of splicing-dependent trans-synaptic adhesion by PTPδ-IL1RAPL1/IL-1RAcP for synaptic differentiation (2015)
  • Author(s): Yamagata A., Yoshida, T., Sato, Y., Goto-Ito, S., Uemura, T., Maeda, A., Shiroshima, T., Iwasawa-Okamoto, S., Mori, H., Mishina, M., Fukai , S.
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 6926-6926
  • DOI: 10.1038/ncomms7926
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structure of Slitrk2-PTPδ complex reveals mechanisms for splicing-dependent trans-synaptic adhesion (2015)
  • Author(s): Yamagata A., Sato, Y., Goto-Ito, S., Uemura, T., Maeda, A., Shiroshima, T., Yoshida, T., Fukai , S.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 9686-9686
  • DOI: 10.1038/srep09686
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural Basis for methyl-donor-dependent and sequence-specific binding to tRNA substrates by knotted methyltransferase TrmD (2015)
  • Author(s): Takuhiro Ito, Isao Masuda, Ken-ichi Yoshida, Sakurako Goto-Ito, Shun-ichi Sekine, Se Won Suh, Ya-Ming Hou, Shigeyuki Yokoyama
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 112 Issue: 31 Pages: 4197-205
  • DOI: 10.1073/pnas.1422981112
  • Peer Reviewed / Open Access / Int'l Joint Research