Establishment of an over-expression system for mammalian large membrane proteins
Project/Area Number |
25650020
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | The University of Tokyo |
Principal Investigator |
Ogawa Haruo 東京大学, 分子細胞生物学研究所, 准教授 (40292726)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
Keywords | 大量発現 / 膜蛋白質 / アデノウィルス / 超巨大蛋白質 |
Outline of Final Research Achievements |
Although the number of atomic structures of membrane proteins deposited in PDB increases year by year, most of them are bacterial homologues of mammalian ones or low molecular-weight ones such as GPCRs. X-ray crystallographic studies of mammalian large membrane proteins have not been conducted well. One of the reasons is that there is no easy as well as versatile system for the protein production of such proteins. Thus, we aimed to establish an over-expression system for mammalian large membrane proteins, such as IP3 receptor, with modifications of our own expression system. The new system should be useful for carrying out kinetic studies for such proteins which could not be studied so far. Moreover, high-resolution strucuture of such proteins should be useful for new drug-development.
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Report
(5 results)
Research Products
(23 results)
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[Journal Article] Genotype-phenotype correlations of malignant hyperthermia and central core disease mutations in the central region of the RYR1 channel.2016
Author(s)
Murayama, T., Kurebayashi, N., Ogawa, H., Yamazawa, T., Oyamada, H., Suzuki, J., Kanemaru, K., Oguchi, K., Iino, M. and Sakurai, T.
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Journal Title
Hum Mutat.
Volume: 37
Issue: 11
Pages: 1231-1241
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Functional analysis of SERCA1b, a highly expressed SERCA1 variant in myotonic dystrophy type 1 muscle2015
Author(s)
Zhao, Y., Ogawa, H., Yonekura, S., Mitsuhashi, H., Mitsuhashi, S., Nishino, I., Toyoshima, C., Ishiura, S.
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Journal Title
Biochim. Biophys. Acta.
Volume: 1852
Issue: 10
Pages: 2042-2047
DOI
Related Report
Peer Reviewed
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[Journal Article] Biselyngbyasides, Cytotoxic Marine Macrolides, are Novel and Potent Inhibitors of the Ca2+ Pumps with a Unique Mode of Binding2015
Author(s)
Morita, M., Ogawa, H., Ohno, O., Yamori, T., Suenaga, K., Toyoshima, C.
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Journal Title
FEBS Lett.
Volume: 589
Issue: 13
Pages: 1406-1411
DOI
Related Report
Peer Reviewed
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[Journal Article] Stimulation, inhibition or stabilization of Na, K-ATPase caused by specific lipid interactions at distinct sites2015
Author(s)
M. Habeck, H. Haviv, A. Katz, E. Kapri-Pardes, S. Ayciriex, A. Shevchenko, H. Ogawa, C. Toyoshima, and S.J.D. Karlish
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Journal Title
J. Biol. Chem.
Volume: 290
Issue: 8
Pages: 4829-4842
DOI
Related Report
Peer Reviewed
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[Presentation] Large Production of SERCA toward determination of three-dimensional structures2014
Author(s)
Haruo Ogawa, Yimeng Zhao, Ayami Hirata, Junko Tsueda, Shoichi Ishiura, Giuseppe Inesi, Chikashi Toyoshima
Organizer
14th International Conference Na,K-ATPase and related transport ATPases: Structure, mechanism, cell biology, health and disease
Place of Presentation
De Werelt Conference Centre, Lunteren, Netherlands
Year and Date
2014-08-30 – 2014-09-05
Related Report
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