| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
Although the number of atomic structures of membrane proteins deposited in PDB increases year by year, most of them are bacterial homologues of mammalian ones or low molecular-weight ones such as GPCRs. X-ray crystallographic studies of mammalian large membrane proteins have not been conducted well. One of the reasons is that there is no easy as well as versatile system for the protein production of such proteins. Thus, we aimed to establish an over-expression system for mammalian large membrane proteins, such as IP3 receptor, with modifications of our own expression system. The new system should be useful for carrying out kinetic studies for such proteins which could not be studied so far. Moreover, high-resolution strucuture of such proteins should be useful for new drug-development.
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