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Structure determination of human membrane transporters

Research Project

Project/Area Number 25650023
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionKyoto University

Principal Investigator

SHIMAMURA Tatsuro  京都大学, 医学(系)研究科(研究院), 講師 (90391979)

Co-Investigator(Kenkyū-buntansha) NOMURA Morimichi  京都大学, 大学院医学研究科, 助教 (10314246)
Co-Investigator(Renkei-kenkyūsha) TANAKA Yoshiki  京都大学, 大学院医学研究科, 研究員 (10632333)
OGASAWARA Satoshi  京都大学, 大学院医学研究科, 研究員 (30546685)
ARAKAWA Takatoshi  京都大学, 大学院医学研究科, 研究員 (30523766)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords膜輸送体 / 結晶構造解析 / 創薬 / 輸送体 / トランスポーター / X線結晶構造解析
Outline of Final Research Achievements

Membrane transporters are drug targets because they are important causes of diseases. Therefore, the structural information of human membrane transporters is very useful for the rational design of new drugs. However, it is difficult to determine the crystal structure of membrane transporters because of their instabilities. We tried to crystallize mammalian transporters using the recently developed methods for the membrane protein crystallization and succeeded to crystallize some of the membrane transporters.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 2013

All Journal Article (7 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Invited: 2 results)

  • [Journal Article] Grease matrix as a versatile carrier of proteins for serial crystallography.2015

    • Author(s)
      Sugahara M, Shimamura T, et al.
    • Journal Title

      Nat Methods.

      Volume: 12 Issue: 1 Pages: 61-63

    • DOI

      10.1038/nmeth.3172

    • NAID

      120005682565

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser.2015

    • Author(s)
      Tono K, Shimamura T, et al.
    • Journal Title

      J. Synchrotron Rad.

      Volume: 22 Issue: 3 Pages: 532-537

    • DOI

      10.1107/s1600577515004464

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteoliposome-based selection of a recombinant antibody fragment against the human M2 muscarinic acetylcholine receptor.2014

    • Author(s)
      Suharni, Nomura Y, Arakawa T, Hino T, Abe H, Nakada-Nakura Y, Sato Y, Iwanari H, Shiroishi M, Asada H, Shimamura T, Murata T, Kobayashi T, Hamakubo T, Iwata S, Nomura N.
    • Journal Title

      Monoclon Antib Immunodiagn Immunother

      Volume: 33 Issue: 6 Pages: 378-385

    • DOI

      10.1089/mab.2014.0041

    • NAID

      120005530933

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of a symmetric football-shaped GroEL:GroES2-ATP14 complex determined at 3.8Å reveals rearrangement between two GroEL rings.2014

    • Author(s)
      Koike-Takeshita A, Arakawa T, Taguchi H, Shimamura T.
    • Journal Title

      J Mol Biol.

      Volume: 426 Issue: 21 Pages: 3634-41

    • DOI

      10.1016/j.jmb.2014.08.017

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] X-ray structure of histamine H1 receptor2014

    • Author(s)
      Shimamura T.
    • Journal Title

      Nihon Yakurigaku Zasshi.

      Volume: 144 Pages: 43-4

    • NAID

      130004437205

    • Related Report
      2014 Annual Research Report
  • [Journal Article] ヒスタミンH1受容体2014

    • Author(s)
      島村達郎
    • Journal Title

      日本薬理学会誌

      Volume: 未定

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] ヒスタミン受容体2013

    • Author(s)
      島村達郎
    • Journal Title

      膜タンパク質構造研究

      Volume: 1 Pages: 11-18

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒスタミンH1受容体の構造解析とin silico screening2014

    • Author(s)
      島村達郎
    • Organizer
      日本内分泌学会学術総会
    • Place of Presentation
      博多
    • Year and Date
      2014-04-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ヒスタミンH1受容体の構造解析とin silico screening2014

    • Author(s)
      島村達郎
    • Organizer
      第87回日本内分泌学会学術総会
    • Place of Presentation
      博多
    • Related Report
      2013 Research-status Report
  • [Presentation] Crystal structure of Histamine H1 receptor and drug design.2013

    • Author(s)
      Shimamura T
    • Organizer
      The 11th International Symposium for Future Drug Discovery and Medical Care.
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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