Construction of assay system for the ligand of G protein-coupled receptor using a fission yeast
| Project/Area Number |
25650032
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Toshiya Osada 東京工業大学, 生命理工学研究科, 准教授 (00201997)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | GPCR / fission yeast / pheromone / GFP / フェロモン / グルコース / 分裂酵母 / リガンドセンサー / 創薬 |
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| Outline of Final Research Achievements |
G-protein-coupled receptors (GPCRs) are seven transmembrane proteins and activated by hormones, odorants, neurotransmitters, and so on. GPCRs are one of the most potential drug targets. However, it is difficult to reproduce functional GPCR expression in heterologous cells due to the unknown mechanism of its trafficking and folding. The purpose of this project is the development of the ligand screening system for orphan GPCR and the construction of biosensor that utilizes the GPCR. The reporter strains for detecting a ligand response for an exogenous GPCR were constructed by using fission yeast. The fission yeast has two GPCR pathway. One is the glucose pathway and the other is the pheromone pathway. In this project, GFP reporter systems for both receptors have been constructed. The both transformed cells showed the ligand dependence activity. To increase the expression of the exogenous GPCR in the cells, the exogenous GPCR was modified with P3 signal derived from the fission yeast.
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Report
(4 results)
Research Products
(11 results)
