Visualizing membrane contact site between the ER and Golgi to analyze the functional kinetics
| Project/Area Number |
25650034
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MAEDA Yusuke 大阪大学, 微生物病研究所, 准教授 (00294124)
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | 膜コンタクト部位 / ゴルジ装置 / コレステロール / スプリットタンパク質 / 膜コンタクト(接触)部位 / オルガネラ / 小胞非依存的輸送 |
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| Research Abstract |
As a first step to visualize speculated membrane contact sites (MCS) between intracellular organelles such as the endoplasmic reticulum and Golgi apparatus, I proved the presence of the sites where these organelles membranes are closely apposed by Proximity Ligation Assay. Next, I tried to establish a split-DHFR system for real-time monitoring of MCS in vivo. At present, fairly good results are obtained and it is expected in near future to achieve the original goal of visualizing MCS in vivo by increasing the sensitivities and to clarify the mechanisms by which the transport of cholesterol is regulated.
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Report
(2 results)
Research Products
(7 results)
