Novel Transport Assay System by SPA Method
| Project/Area Number |
25650035
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Okayama University |
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Principal Investigator |
OMOTE Hiorshi 岡山大学, 医歯(薬)学総合研究科, 准教授 (10273707)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | トランスポーター / 輸送活性測定 / リポソーム / spa / SPA / 再構成リポソーム / SPA法 / 活性測定 |
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| Outline of Final Research Achievements |
Membrane transporters play essential roles in various cellular activities such as nutrient uptake, control of drug concentration and neuronal signal transmission. Therefore, measurement of transport activity is important for basic biology, medical science and pharmacology. So far, function of transporters is measured using heterologous expression system. However, it has difficulty due to artifact by other transporters presented in the cells.
In this study, we have aimed to develop novel transport assay system with SPA method. In this system, purified transporter is embedded in the liposomes and attached to SPA scintillation beads through appropriate tags. We found that this technique is useful for future transporter study.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Essential role of vesicular nucleotide transporter in vesicular storage and release of nucleotides in platelets.2014
Author(s)
Hiasa, M., Togawa, N., Miyaji, T., Omote, H. Yamamoto, A., Moriyama, Y
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Journal Title
Related Report
Peer Reviewed
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