| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
In the light conditions, photosynthetic systems produce NADPH and ATP in higher plants. NADPH and ATP are further used for the reduction of CO2 in the chloroplast stroma. In this study, we proposed to develop NADPH sensor by fluorescent energy transfer (FRET) using NADPH-binding protein and Green fluorescent protein (GFP)-derivatives.
We constructed the fused proteins which CFP (GFP-derivative) protein was fused at N-terminus of NADPH-binding protein and YFP (GFP-derivative) protein was fused at C-termius of NADPH-binding protein. The fusion-proteins were overexpressed.
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