Validation of hypothesis for "fucose-dependent polarized transport system" by visualizing specific glycoforms of glycoproteins
| Project/Area Number |
25650041
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
SUZUKI Tadashi 独立行政法人理化学研究所, 糖鎖代謝学研究チーム, チームリーダー (90345265)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
芳賀 淑美 理化学研究所, 糖鎖代謝学研究チーム, 特別研究員 (40525789)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | AFP / 糖鎖 / アピカル / 極性輸送 / フコース / イメージング / α-フェトプロテイン |
|---|
| Outline of Final Research Achievements |
Previously we have established a novel method for visualization of glycoforms of specific glycoforms of a protein of interest. This research proposal aimed to clarify the molecular mechanism of fucose-dependent polarized transport of alpha-fetoprotein (AFP) in hepatocytes by utilizing the new technique we developed. While we could successfully establish the expression system of AFP in hepatocyte-derived HepG2 cells, we had difficulty to find a conditions to allow cells to incorporate azide-modified fucose in cells. In the future, we need to establish (1) more efficienct transfection method for AFP and (2) improvement of incorporation efficiency for azide-fucose into HepG2 cells to establish the assay method for polarized transport of AFP.
|
Report
(3 results)
Research Products
(13 results)
