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Validation of hypothesis for "fucose-dependent polarized transport system" by visualizing specific glycoforms of glycoproteins

Research Project

Project/Area Number 25650041
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SUZUKI Tadashi  独立行政法人理化学研究所, 糖鎖代謝学研究チーム, チームリーダー (90345265)

Co-Investigator(Renkei-kenkyūsha) 芳賀 淑美  理化学研究所, 糖鎖代謝学研究チーム, 特別研究員 (40525789)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsAFP / 糖鎖 / アピカル / 極性輸送 / フコース / イメージング / α-フェトプロテイン
Outline of Final Research Achievements

Previously we have established a novel method for visualization of glycoforms of specific glycoforms of a protein of interest. This research proposal aimed to clarify the molecular mechanism of fucose-dependent polarized transport of alpha-fetoprotein (AFP) in hepatocytes by utilizing the new technique we developed. While we could successfully establish the expression system of AFP in hepatocyte-derived HepG2 cells, we had difficulty to find a conditions to allow cells to incorporate azide-modified fucose in cells. In the future, we need to establish (1) more efficienct transfection method for AFP and (2) improvement of incorporation efficiency for azide-fucose into HepG2 cells to establish the assay method for polarized transport of AFP.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2015 2014 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Invited: 2 results) Book (3 results)

  • [Journal Article] Use of transmembrane FRET to investigate the internalization of glycosylated proteins.2014

    • Author(s)
      Yoshimi Haga and Tadashi Suzuki
    • Journal Title

      Methods in Molecular Biology

      Volume: 1174 Pages: 225-230

    • DOI

      10.1007/978-1-4939-0944-5_15

    • ISBN
      9781493909438, 9781493909445
    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 特定の糖鎖構造をもつ標的タンパク質の蛍光イメージングと細胞内動態解析2014

    • Author(s)
      芳賀 淑美、鈴木 匡
    • Journal Title

      化学と生物

      Volume: 52 Pages: 100-105

    • NAID

      130004808945

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
  • [Presentation] Glycoform imaging: use of transmembrane FRET to investigate the internalization of glycosylated proteins.2014

    • Author(s)
      Yoshimi Haga, Kumiko Ishii, Kayo Hibino, Yasuhi Sako, Yukishige Ito, Naoyuki Taniguchi, and Tadashi Suzuki
    • Organizer
      Joint Meeting of the Society for Glycobiology and the Japanese Society of Carbohydrate Research
    • Place of Presentation
      Honolulu, Hawaii
    • Year and Date
      2014-11-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Glycoform imaging: use of transmembrane FRET to investigate the internalization of glycosylated proteins.2014

    • Author(s)
      Yoshimi Haga, Kumiko Ishii, Kayo Hibino, Yasuhi Sako, Yukishige Ito, Naoyuki Taniguchi, and Tadashi Suzuki
    • Organizer
      2014 SFG & JSCR Joint Meeting Satellite Symposium II Glycans in Neuroscience
    • Place of Presentation
      Honolulu, Hawaii
    • Year and Date
      2014-11-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Exploring the “life” of glycans.2013

    • Author(s)
      Tadashi Suzuki
    • Organizer
      RIKEN-Max Planck Joint Research Center for Systems Chemical Biology The Second Symposium
    • Place of Presentation
      理化学研究所(和光)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Imaging of glycoforms as a sweet tool to visualize the dynamics of specific glycoproteins.2013

    • Author(s)
      Yoshimi Haga, Kumiko Ishii, Kayo Hibino, Yasushi Sako, Yukishige Ito, Naoyuki Taniguchi, and Tadashi Suzuki
    • Organizer
      RIKEN-Max Planck Joint Research Center for Systems Chemical Biology The Second Symposium
    • Place of Presentation
      理化学研究所(和光)
    • Related Report
      2013 Research-status Report
  • [Presentation] Imaging of glycoforms as a sweet tool to visualize the dynamics of specific glycoprpoteins.2013

    • Author(s)
      Yoshimi Haga, Kumiko Ishii, Kayo Hibino, Yasushi Sako, Yukishige Ito, Naoyuki Taniguchi, and Tadashi Suzuki
    • Organizer
      The 3rd Austria/Japan Seminar on Comparative and Developmental Glycobiology
    • Place of Presentation
      理化学研究所(和光)
    • Related Report
      2013 Research-status Report
  • [Presentation] 標的タンパク質の糖鎖修飾蛍光イメージングと細胞内動態解析2013

    • Author(s)
      芳賀淑美、石井久美子、日比野佳代、佐甲靖志、伊藤幸成、谷口直之、鈴木 匡
    • Organizer
      第32回日本糖質学会年会
    • Place of Presentation
      大阪国際交流センター(大阪)
    • Related Report
      2013 Research-status Report
  • [Presentation] 特定の糖鎖構造を持つ標的タンパク質の蛍光イメージングと細胞内動態解析2013

    • Author(s)
      芳賀淑美、石井久美子、日比野佳代、佐甲靖志、伊藤幸成、谷口直之、鈴木 匡
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Related Report
      2013 Research-status Report
  • [Presentation] 糖鎖の一生を追いかける

    • Author(s)
      鈴木 匡
    • Organizer
      第5回岡山大&理研ジョイントシンポジウム 最先端計測技術のトレンド
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] 糖鎖の蛍光イメージング In 糖鎖の新機能開発応用ハンドブック (秋吉一成 監修)2015

    • Author(s)
      鈴木 匡
    • Publisher
      NTS出版
    • Related Report
      2014 Annual Research Report
  • [Book] Glycoform Imaging. In Glycoscience: Biology and Medicine. (N. Taniguchi, T. Endo, G. W. Hart, P. Seeberger, C-H. Wong eds.)2014

    • Author(s)
      Yoshimi Haga and Tadashi Suzuki
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] FRETを使った特定糖タンパク質の可視化 (pp186-191) 第三の生命鎖研究の最前線 糖鎖の機能・作動原理と疾患 実験医学(門松 健治、遠藤 玉夫、岡 昌吾、北川 裕之 編)2013

    • Author(s)
      芳賀 淑美、鈴木 匡
    • Total Pages
      225
    • Publisher
      実験医学
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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