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Elucidation of the antigen-presenting mechanism of gold nanoparticles functioning as antibody-producing carriers

Research Project

Project/Area Number 25650042
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

ISHII NORIYUKI  国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (10261174)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords免疫生化学 / 抗ハプテン抗体 / 金ナノ微粒子担体 / 株化培養細胞 / ナノ粒子 / 細胞応答 / ハプテン / B細胞 / 抗体 / エキソソーム / 細胞外膜小胞 / 培養細胞株 / 抗原抗体反応 / 抗原 / B細胞
Outline of Final Research Achievements

In vivo production of anti-hapten antibody has been confirmed previously by the use of gold nanoparticles capped with small chemical substances as a starting point. The present molecular and cellular level study concludes that the gold nanoparticles bound to the chemical substances as an immunogen are at first, engulfed by macrophages and dendritic cells, and then intracellularly digested in the cytoplasm due to its reduced condition. The immunogen is then transported from early endosome through multivesicular body, and is released into the extracellular in the form that is contained in exosomes. It is proper to consider such exosomes activate B-cells. The MHC molecules are known to exist in the exosomes released from dendritic cells, but not detected thus far in the exosomes from macrophages. Very little is currently known about whether the information of the immunogen is delivered from macrophages or dendritic cells, so it is hoped that future research will clarify this point.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 Other

All Presentation (5 results) (of which Invited: 1 results) Book (1 results)

  • [Presentation] エキソソームって何? 十万倍で見る生体の謎2015

    • Author(s)
      石井 則行、池本 光志、広瀬 恵子、小田原 孝行
    • Organizer
      2015年 産総研(つくばセンター)サイエンスコーナー
    • Place of Presentation
      茨城県・つくば市
    • Year and Date
      2015-07-18
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] タンパク質会合における混み合った分子環境の役割2015

    • Author(s)
      小田原孝行、池本光志、広瀬恵子、石井則行
    • Organizer
      産総研・産技連LS-BT合同研究発表会
    • Place of Presentation
      産業技術総合研究所(つくば)
    • Year and Date
      2015-02-03
    • Related Report
      2014 Research-status Report
  • [Presentation] Novel electron microscopic elemental technology to support the development of exosomes as disease diagnostic markers2015

    • Author(s)
      石井則行、池本光志、広瀬恵子、小田原孝行
    • Organizer
      産総研・産技連LS-BT合同研究発表会
    • Place of Presentation
      産業技術総合研究所(つくば)
    • Year and Date
      2015-02-03
    • Related Report
      2014 Research-status Report
  • [Presentation] Immunological study with azobenzene-dye as a hapten presented on the surface of gold nanoparticle carriers

    • Author(s)
      石井則行、玉田薫、秋山陽久
    • Organizer
      生物物理学会
    • Place of Presentation
      京都国際会館(京都)
    • Related Report
      2013 Research-status Report
  • [Presentation] 金ナノ粒子キャリヤー表面に提示されたハプテンとしてのアゾベンゼン色素の免疫応答

    • Author(s)
      石井則行、玉田薫、秋山陽久
    • Organizer
      産総研・産技連 LS-BT合同研究発表会
    • Place of Presentation
      産業技術総合研究所(つくば)
    • Related Report
      2013 Research-status Report
  • [Book] Image Analyses of Two-Dimensional Crystalline Arrays of Membrane Proteins and Protein Supramolecular Complexes, In: Advances in Image Analysis Research2014

    • Author(s)
      Noriyuki Ishii
    • Total Pages
      388
    • Publisher
      Nova Science Publishers, Inc.
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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