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Construction of soft nano-device using DNA-protein hybrid nano-system

Research Project

Project/Area Number 25650045
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionKyoto University (2014)
The University of Tokyo (2013)

Principal Investigator

TADAKUMA Hisashi  京都大学, 物質-細胞統合システム拠点, 研究員 (10339707)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords1分子計測・操作 / ナノマシン / 蛋白質 / 核酸 / 1分子計測(SMD) / 分子機械 / マイクロ・ナノデバイス
Outline of Final Research Achievements

Toward the handheld device, downsizing of the subcomponent system is the key. For such purpose, integration of proteins, which is an efficient nano-machine, is a prominent approach. For the first step, we integrated RNA polymerase (RNAP) and gene on the DNA-origami base nano-chip, allowing us to rationally design the orthogonal gene nano-chip.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2014 2013

All Presentation (12 results) (of which Invited: 3 results)

  • [Presentation] 生物遺伝子発現システムを集積化したバイオナノチップの構築に向けて2014

    • Author(s)
      増渕岳也、多田隈尚史、遠藤政幸、原田慶恵、杉山弘、上田卓也
    • Organizer
      計測自動制御学会 システム・情報部門学術講演会 2014 (SSI2014)
    • Place of Presentation
      岡山大学 創立五十周年記念館
    • Year and Date
      2014-11-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Rational design of orthogonal gene transcription nano device on DNA origami2014

    • Author(s)
      Masubuchi T, Tadakuma H, Endo M, Sugiyama H, Harada Y, Ueda T
    • Organizer
      第87回日本生化学会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Cross-linking the dynein-microtubule complex by DNA origami2014

    • Author(s)
      広瀬恵子, 厳康敏, 多田隈尚史
    • Organizer
      第52回日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Rational design of orthogonal gene transcription nano device on DNA origami2014

    • Author(s)
      Masubuchi T, Tadakuma H, Endo M, Sugiyama H, Harada Y, Ueda T
    • Organizer
      第52回日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA origamiを用いた直交性のある転写ナノデバイスの構築2014

    • Author(s)
      Masubuchi T, Tadakuma H, Endo M, Sugiyama H, Harada Y, Ueda T
    • Organizer
      第8回バイオ関連化学シンポジウム
    • Place of Presentation
      岡山大学津島キャンパス
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA origamiを用いた直交性のある転写ナノデバイスの構築2014

    • Author(s)
      Masubuchi T, Tadakuma H, Endo M, Sugiyama H, Harada Y, Ueda T
    • Organizer
      第16回RNA学会
    • Place of Presentation
      名古屋市・ウインクあいち
    • Year and Date
      2014-07-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNAナノ構造を用いたナノ転写チップの構築: ナノ反応場における転写活性2014

    • Author(s)
      多田隈尚史
    • Organizer
      第66回日本細胞生物学会大会
    • Place of Presentation
      奈良県新公会堂
    • Year and Date
      2014-06-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] High-resolution Thermodynamic Analysis of ABC-Transporter MsbA Reconstituted in Nanodiscs.2014

    • Author(s)
      Kawai T, Tadakuma H, Caaveiro JMM, Tsumoto K, Ueda T
    • Organizer
      Biophysical meeting
    • Place of Presentation
      Moscone Center (米国・CA)
    • Related Report
      2013 Research-status Report
  • [Presentation] Construction and functional analysis of DNA origami base DNA-RNAP hybrid nanomachine2013

    • Author(s)
      Masubuchi T, Tadakuma H, Endo M, Sugiyama H, Harada Y, Ueda T
    • Organizer
      日本生物物理学会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2013 Research-status Report
  • [Presentation] Designing the nano-reaction field: Introduction and application to motor protein research2013

    • Author(s)
      Tadakuma H
    • Organizer
      日本生物物理学会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Construction and functional analysis of DNA origami base DNA-RNAP hybrid nanomachine2013

    • Author(s)
      Masubuchi T, Tadakuma H, Endo M, Sugiyama H, Harada Y, Ueda T
    • Organizer
      CBI学会
    • Place of Presentation
      タワーホール岩堀(東京都)
    • Related Report
      2013 Research-status Report
  • [Presentation] ナノ反応場デザインによるDNA-モーター蛋白質ハイブリッドの自律運動設計に向けて2013

    • Author(s)
      多田隈 尚史
    • Organizer
      「細胞を創る」研究会6.0
    • Place of Presentation
      慶応大学・鶴岡キャンパス
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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