• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Noninvasive Single molecule imaging in mice

Research Project

Project/Area Number 25650047
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionThe University of Tokyo

Principal Investigator

HIGUCHI Hideo  東京大学, 理学(系)研究科(研究院), 教授 (90165093)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords量子ドット / 高輝度 / マウス / 非侵襲 / in vivo / イメージング / 1粒子
Outline of Final Research Achievements

To image the single molecule in vivo, we prepared very bright multiple quantum dots including several particles of quantum dots The solution of quantum dots was rapidly frozen in liquid nitrogen. The dots were aggregated in the frozen process. The number of quantum dots including in the multiple quantum dots were 10-100. The multiple quantum dots bound antibodies were bound to living cells. The bright fluorescence image was obtained.
We developed new imaging methods to visualize molecules under noninvasive condition. We developed new imaging methods to visualize molecules under noninvasive condition. Tumor was successfully formed using several cells line. To image the molecules, specific antibodies to recognize these cells were labeled with fluorescence quantum dots and then injected to tail vein after the formation of tumor. We successfully performed real time observation of quantum dots within breast cancer cells and on its membrane under noninvasive condition.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Invited: 2 results)

  • [Journal Article] Nano-scale measurement of biomolecules by optical microscopy and semiconductor nanoparticles2014

    • Author(s)
      Taro Ichimura, Takashi Jin, Hideaki Fujita, Hideo Higuchi, Tomonobu M. Watanabe
    • Journal Title

      Front. Physiol., fphys.

      Volume: 5 Pages: 1-13

    • DOI

      10.3389/fphys.2014.00273

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Noninvasive in vivo imaging of neutrophil and tumor in mouse auricles2014

    • Author(s)
      Hideo Higuchi, Kenji Kikushima and Sayaka Kita
    • Organizer
      8th Internal Symposium on Nanomedicine
    • Place of Presentation
      愛媛県 松山市
    • Year and Date
      2014-12-07 – 2014-12-08
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 蛍光量子ドットを用いた細胞内高速小胞輸送機構の解明2014

    • Author(s)
      菊島健児、樋口秀男
    • Organizer
      日本生物物理学会年会,
    • Place of Presentation
      北海道 札幌市
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 蛍光量子ドットを用いた細胞内高速小胞輸送機構の解明2014

    • Author(s)
      菊島健児、樋口秀男
    • Organizer
      ナノ学会
    • Place of Presentation
      京都府 京都
    • Year and Date
      2014-05-22 – 2014-05-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] non-invasive technique for the in vivo tracking of high-speed vesicle transport in mouse neutrophils2013

    • Author(s)
      菊島健児, 喜多清, 樋口秀男
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2013 Research-status Report
  • [Presentation] Noninvasive in vivo imaging of neutrophil and tumor in mouse auricles2013

    • Author(s)
      樋口秀男,菊島健児, 喜多清
    • Organizer
      Dynein 2013 International Workshop
    • Place of Presentation
      オルビスホール(兵庫)
    • Related Report
      2013 Research-status Report
    • Invited

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi