Force Transmission Mechanism by Dynein Molecules That Controls Ciliary Beating Movement
Project/Area Number |
25650048
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Biophysics
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Research Institution | The University of Tokyo |
Principal Investigator |
TOYOSHIMA Yoko 東京大学, 総合文化研究科, 教授 (40158043)
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Co-Investigator(Kenkyū-buntansha) |
YAJIMA Junichiro 東京大学, 大学院総合文化研究科, 准教授 (00453499)
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Research Collaborator |
SUGAWA Mitsuhiro
KOBAYASHI Takuya
ICHIKAWA Muneyoshi
YAMAGUCHI Shin
SAITO Kei
KOISO Yurika
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 繊毛運動 / 軸糸 / 微小管 / ダイニン / 基底小体 / 繊毛打運動 / テトラヒメナ / 軸糸ダイニン / テトラヒメナ組換え体 / 遺伝子導入 / 3次元位置計測 |
Outline of Final Research Achievements |
We have developed an optical microscopic imaging system that is able to observe the 3-dimentional motion of individual cilia in Tetrahymena thermophile with high spatial precision, and that detected the beating pattern. The genes encoding axonemal dyneins, dynein binding proteins and basal body localized proteins fused to EGFP were successfully translated into Tetrahymna. Our results suggest that responses of ciliary movement depending on dynein activities localized to the axoneme are quantified and therefore dynein functions in a ciliary motion system can be quantified.
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Report
(4 results)
Research Products
(25 results)
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[Presentation] Axonemal Dynein Light Chain-1 Locates at the Microtubule Binding Domain of the γ Heavy Chain2015
Author(s)
Muneyoshi Ichikawa M., Saito K., Yanagisawa H., Yagi, T., Kamiya, R., Yamaguchi S., Yajima, J., Kushida Y., Nakano, K., Numata, O. and Toyoshima, Y. Y.
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学(石川県金沢市)
Year and Date
2015-09-13
Related Report
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[Presentation] Off-axis motion of yeast cytoplasmic dynein takes a biased random walk2015
Author(s)
Sugawa, M., Yamaguchi, S., Takagi, H., Shibata, K., Toyoshima, Y. Y., Yajima, J.
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学(石川県金沢市)
Year and Date
2015-09-13
Related Report
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[Presentation] Dynactin is a biphasic regulator to promote and inhibit dynein motility2015
Author(s)
Kobayashi, K., Miyashita, K., Kajita, H., Saito, K., Murayama, T., Toyoshima, Y. Y.
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学(石川県金沢市)
Year and Date
2015-09-13
Related Report
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[Presentation] LC1 binds to the stalk of the outer arm dynein2014
Author(s)
Muneyoshi Ichikawa, kei Saito, Haru-aki Yanagisawa, Toshiki Yagi, Ritsu Kamiya, Yasuhar Kushida, Kentaro Nakano, Osamu Numata, Yoko Y. Toyoshima
Organizer
第52回日本生物物理学会年会
Place of Presentation
札幌コンベンションセンター(北海道)
Year and Date
2014-09-13 – 2014-09-15
Related Report
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[Presentation] Self-regulation of cytoplasmic dynein2013
Author(s)
Ken’ya Furuta, Takayuyki Torisawa, Akane Furuta, Muneyoshi Ichikawa, Kei Saito, Kazuhiro Oiwa, Hiroaki Kojima, Yoko Toyoshima
Organizer
International Workshop Dynein2013
Place of Presentation
兵庫県、神戸オルビスホール
Related Report
Invited
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[Presentation] Self-regulation of cytoplasmic dynein2013
Author(s)
Ken'ya Furuta, Takayuki Torisawa, Akane Furuta, Muneyoshi Ichikawa, Kei Saito, Kauzuhiro Oiwa, Hiroaki Kojima, Yoko Toyoshima
Organizer
American Society for Cell Biology 2013 Annual Meeting
Place of Presentation
New Orleans, USA
Related Report
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