Force Transmission Mechanism by Dynein Molecules That Controls Ciliary Beating Movement

• Project/Area Number: 25650048
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: TOYOSHIMA Yoko 東京大学, 総合文化研究科, 教授 (40158043)
• Co-Investigator(Kenkyū-buntansha): YAJIMA Junichiro 東京大学, 大学院総合文化研究科, 准教授 (00453499)
• Research Collaborator: SUGAWA Mitsuhiro ; KOBAYASHI Takuya ; ICHIKAWA Muneyoshi ; YAMAGUCHI Shin ; SAITO Kei ; KOISO Yurika
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 繊毛運動 / 軸糸 / 微小管 / ダイニン / 基底小体 / 繊毛打運動 / テトラヒメナ / 軸糸ダイニン / テトラヒメナ組換え体 / 遺伝子導入 / 3次元位置計測

Outline of Final Research Achievements

We have developed an optical microscopic imaging system that is able to observe the 3-dimentional motion of individual cilia in Tetrahymena thermophile with high spatial precision, and that detected the beating pattern. The genes encoding axonemal dyneins, dynein binding proteins and basal body localized proteins fused to EGFP were successfully translated into Tetrahymna. Our results suggest that responses of ciliary movement depending on dynein activities localized to the axoneme are quantified and therefore dynein functions in a ciliary motion system can be quantified.

Research Products

• [Journal Article] Axonemal dynein light chain-1 locates at the microtubule-binding domain of the γ heavy chain. (2015)
  • Author(s): Ichikawa M, Saito K, Yanagisawa HA, Yagi T, Kamiya R, Yamaguchi S, Yajima J, Kushida Y, Nakano K, Numata O and Toyoshima YY.
  • Journal Title: Molicular Biology of the Cell Volume: 26 Issue: 23 Pages: 4236-47
  • DOI: 10.1091/mbc.e15-05-0289
  • Peer Reviewed / Open Access
• [Journal Article] α- and β-tubulin lattice arrangement of the axonemal microtubule doublet and binding proteins revealed by single particle cryo-electron microscopy and tomography. (2015)
  • Author(s): Maheshwari, A., Obbineni, J. M., Bui, K. H., Shibata, K., Toyoshima Y. Y. & Ishikawa, T.
  • Journal Title: Structure Volume: 23 Issue: 9 Pages: 1584-1595
  • DOI: 10.1016/j.str.2015.06.017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regulatory Mechanism of Cytoplasmic Dynein (2015)
  • Author(s): 鳥澤嵩征、古田健也、豊島陽子
  • Journal Title: Seibutsu Butsuri Volume: 55 Issue: 3 Pages: 127-132
  • DOI: 10.2142/biophys.55.127
  • NAID: 130005072019
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed / Open Access
• [Journal Article] Torque Generation by Axonemal Outer-Arm Dynein (2014)
  • Author(s): Shin Yamaguchi, Kei Saito, Miki Sutoh, Takayuki Nishizaka, Yoko Y Toyoshima, Junichiro Yajima
  • Journal Title: Biophysical Journal Volume: 108 Issue: 4 Pages: 872-879
  • DOI: 10.1016/j.bpj.2014.12.038
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sea lily muscle lacks a troponin-regulatory system but contains paramyosin. (2014)
  • Author(s): Obinata T, Amemiya S, Takai R, Ichikawa M, Toyosima YY, Sato N
  • Journal Title: Zoological Science Volume: 31 Issue: 3 Pages: 122-128
  • DOI: 10.2108/zsj.31.122
  • Peer Reviewed
• [Journal Article] Measuring Collective Transport by Defined Numbers of Processive and Nonprocessive Kinesin Motors (2013)
  • Author(s): 古田 健也, 古田 茜, 豊島 陽子, 網野 美紗子, 大岩 和弘, 小嶋 寛明
  • Journal Title: Proc Natl Acad Sci U S A ( 米国科学アカデミー紀要) Volume: 110(2) Issue: 2 Pages: 501-6
  • DOI: 10.1073/pnas.1201390110
  • Peer Reviewed
• [Journal Article] Formation of Ring-Shaped Microtubule Assemblies through Active Self-Organization on Dynein (2013)
  • Author(s): Masaki Ito, Arif Md Rashedul Kabir, Daisuke Inoue, Takayuki Torisawa, Yoko Toyoshima, Kazuki Sada, Akira Kakugo
  • Journal Title: Polymer Journal Volume: 46 Issue: 4 Pages: 220-225
  • DOI: 10.1038/pj.2013.89
  • NAID: 120005515308
  • Peer Reviewed
• [Presentation] Axonemal Dynein Light Chain-1 Locates at the Microtubule Binding Domain of the γ Heavy Chain (2015)
  • Author(s): Muneyoshi Ichikawa M., Saito K., Yanagisawa H., Yagi, T., Kamiya, R., Yamaguchi S., Yajima, J., Kushida Y., Nakano, K., Numata, O. and Toyoshima, Y. Y.
  • Organizer: 第５３回日本生物物理学会年会
  • Place of Presentation: 金沢大学（石川県金沢市）
  • Year and Date: 2015-09-13
• [Presentation] Off-axis motion of yeast cytoplasmic dynein takes a biased random walk (2015)
  • Author(s): Sugawa, M., Yamaguchi, S., Takagi, H., Shibata, K., Toyoshima, Y. Y., Yajima, J.
  • Organizer: 第５３回日本生物物理学会年会
  • Place of Presentation: 金沢大学（石川県金沢市）
  • Year and Date: 2015-09-13
• [Presentation] Dynactin is a biphasic regulator to promote and inhibit dynein motility (2015)
  • Author(s): Kobayashi, K., Miyashita, K., Kajita, H., Saito, K., Murayama, T., Toyoshima, Y. Y.
  • Organizer: 第５３回日本生物物理学会年会
  • Place of Presentation: 金沢大学（石川県金沢市）
  • Year and Date: 2015-09-13
• [Presentation] Torque generation by axonemal outer-arm dynein (2014)
  • Author(s): Shin Yamaguchi, Kei saito, Miki Sutoh, Takayuki Nishizaka, Yoko Y Toyoshima, Junichiro Yajima
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2014-09-13 – 2014-09-15
• [Presentation] LC1 binds to the stalk of the outer arm dynein (2014)
  • Author(s): Muneyoshi Ichikawa, kei Saito, Haru-aki Yanagisawa, Toshiki Yagi, Ritsu Kamiya, Yasuhar Kushida, Kentaro Nakano, Osamu Numata, Yoko Y. Toyoshima
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2014-09-13 – 2014-09-15
• [Presentation] New Folding Pattern of p150 and “antenna” for dynein binding (2014)
  • Author(s): Kei Saito, Takashi Murayama, Tomonori Hata, Takuya Kobayashi, Yoko Toyoshima
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] Self-regulation of cytoplasmic dynein through its unconventional force response. (2014)
  • Author(s): Takayuki Torisawa, Ken’ya Furuta, Akane Furuta, Muneyoshi Ichikawa, Kei Saito, Kazuhiro Oiwa, Hiroaki Kojima, Yoko Toyoshima
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] The mechanism of determining the directionality of Ncd (2014)
  • Author(s): Masahiko Yamagishi, Yoko Toyoshima, Junichiro Yajima
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] Torque generation of axonemal outer arm dyneins (2014)
  • Author(s): Shin Yamaguchi, Kei Saito, Miki Sutoh, Takayuki Nishizaka, Yoko Y Toyoshima, Junichiro Yajima,
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] Autoinhibition and synergistid activation of cytoplasmic dynein (2013)
  • Author(s): Takayuki Torisawa, Ken’ya Furuta, Akane Furuta, Muneyoshi Ichikawa, Yoko Toyoshima
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都府、京都国際会議場
• [Presentation] Regulatory mechanism of mictrotubule –based molecular motors by Bicaudal-D2 (2013)
  • Author(s): Takuya Kobayashi, Akira Hanashima, Yoko Toyoshima, Takashi Murayama
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都府、京都国際会議場
• [Presentation] Molecular architecture of dynactin p150 (2013)
  • Author(s): Kei Saito, Takashi Murayama, Tomonori Hata, Yoko Toyoshima
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都府、京都国際会議場
• [Presentation] Subunit structure of Tetrahymena outer dynein arm complex (2013)
  • Author(s): Muneyoshi Ichikawa, Yasuharu Kushida, Kentaro Nakano, Osamu Numata, Yoko Toyoshima
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都府、京都国際会議場
• [Presentation] Torque generation by axonemal outer-arm dynein (2013)
  • Author(s): Shin Yamaguchi, Yoko Toyoshima, Junichiro Yajima
  • Organizer: International Workshop Dynein2013
  • Place of Presentation: 兵庫県、神戸オルビスホール
  • Invited
• [Presentation] Submolecular structure of cytoplasmic dynein tail and dynactin sidearm. (2013)
  • Author(s): Kei Saito, Yuta Watahabe, Muneyoshi Ichikawa, Tomonori Hata, Takuya Kobayashi, Takashi Murayama, Yoko Toyoshima
  • Organizer: International Workshop Dynein2013
  • Place of Presentation: 兵庫県、神戸オルビスホール
  • Invited
• [Presentation] Self-regulation of cytoplasmic dynein (2013)
  • Author(s): Ken’ya Furuta, Takayuyki Torisawa, Akane Furuta, Muneyoshi Ichikawa, Kei Saito, Kazuhiro Oiwa, Hiroaki Kojima, Yoko Toyoshima
  • Organizer: International Workshop Dynein2013
  • Place of Presentation: 兵庫県、神戸オルビスホール
  • Invited
• [Presentation] Self-regulation of cytoplasmic dynein (2013)
  • Author(s): Ken'ya Furuta, Takayuki Torisawa, Akane Furuta, Muneyoshi Ichikawa, Kei Saito, Kauzuhiro Oiwa, Hiroaki Kojima, Yoko Toyoshima
  • Organizer: American Society for Cell Biology 2013 Annual Meeting
  • Place of Presentation: New Orleans, USA
• [Presentation] Submolecular structure of dynactin sidearm: a new folding pattern of p150 (2013)
  • Author(s): Kei Saito, Tomonori Hata, Takuya Kobayashi, Takashi Murayama, Yoko Toyoshima
  • Organizer: American Society for Cell Biology 2013 Annual Meeting
  • Place of Presentation: New Orleans, USA