Correlation between cell migration and differentiation
| Project/Area Number |
25650049
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Nagaoka University of Technology |
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Principal Investigator |
OHNUMA Kiyoshi 長岡技術科学大学, 工学(系)研究科(研究院), 准教授 (50396834)
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| Co-Investigator(Renkei-kenkyūsha) |
TOYOTA Taro 東京大学, 大学院総合文化研究科 (80422377)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 細胞 / ゆらぎ / 幹細胞 / 分化 / 細胞運動 |
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| Outline of Final Research Achievements |
Human induced pluripotent stem cells (hiPSCs) have the ability to differentiate into all types of cells. Letting many types of cells wander around culture dish could lead to out of control behavior, and there are still unknown differences in the movement of various cell types derived from hiPSCs.
In this study, we attempted to restrict the movement of hiPSCs to a specific pattern and to observe cell movement under multiple conditions simultaneously. We succeeded in creating a hair-thin (200 μm in diameter) circular pattern of hiPSCs. We also discovered that the early differentiating hiPSCs move slower than the undifferentiated hiPSCs.
The knowledge about cell movement and the development of the cell-movement regulation technique contribute to future regenerative medical applications.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Enzyme-free Passage of Human Pluripotent Stem Cells by Controlling Divalent Cations.2014
Author(s)
Ohnuma K, Fujiki A, Yanagihara K, Tachikawa S, Hayashi Y, Ito Y,Onuma Y, Chan T, Michiue T, Furue MK, Asashima M
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Journal Title
SCIENTIFIC REPORTS
Volume: 4
Issue: 1
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Microfluidic perfusion culture of human induced pluripotent stem cells under fully defined culture conditions2014
Author(s)
Yoshimitsu, R., K. Hattori, S. Sugiura, Y. Kondo, R. Yamada, S. Tachikawa, T. Satoh, A. Kurisaki, K. Ohnuma, M. Asashima and T. Kanamori
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Journal Title
Biotechnology and Bioengineering
Volume: 111
Issue: 5
Pages: 937-947
DOI
Related Report
Peer Reviewed
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[Presentation] Discs of human induced pluripotent stem cells on a plasma-patterned polydimethylsiloxane surface following single-step coating with vitronectin and γ-globulin2014
Author(s)
Y. Yamamoto, R. Yamada, K. Hattori, S. Tachikawa, M. Tagaya, T. Sasaki, D. Miyamoto, K. Nakazawa, S. Sugiura, T. Kanamori, and K. Ohnuma
Organizer
micro TAS 2014
Place of Presentation
San Antonio, Texas, USA
Year and Date
2014-10-26 – 2014-10-30
Related Report
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