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Development of the microscopy for optical sectioning chemiluminescence imaging

Research Project

Project/Area Number 25650052
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionOsaka University

Principal Investigator

Arai Yoshiyuki  大阪大学, 産業科学研究所, 助教 (20444515)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsイメージング / 化学発光 / 3次元 / 顕微鏡 / 球面収差
Outline of Final Research Achievements

Chemiluminescence imaging enable us excitation light free imaging since the chemiluminescencent probes can emit the light by the reaction with the chemiluminescent substrates. However, three dimensional imaging cannot be done because the system do not use illumination optical system. Here I develop the microscopy by utilizing the spherical aberration of the optical lens for three dimensional chemiluminescence imaging. Although my system could not be used for the extraction of optical plane of chemiluminescence image, the system might have the possibility for other fluorescence imaging system.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2017 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (1 results)

  • [Journal Article] CCDカメラのデッドタイムを利用した光刺激法が可能にする蛍光・化学発光イメージングとオプトジェネティクスの高時間分解併用2014

    • Author(s)
      新井由之、永井健治
    • Journal Title

      生化学

      Volume: 86 Pages: 167-173

    • Related Report
      2014 Research-status Report
  • [Journal Article] Improved orange and red Ca2+ indicators and photophysical considerations for optogenetic applications.2013

    • Author(s)
      Wu JH, Liu L, Matsuda T, Zhao YG, Rebane A, Drobijev M, Chang YF Araki S, Arai Y, March K, Sagou K, Miyata T, Nagai T, Li WH, Campbell R,
    • Journal Title

      ACS Chen. Neurosci.

      Volume: 4 Issue: 6 Pages: 963-972

    • DOI

      10.1021/cn400012b

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation2013

    • Author(s)
      K. Takemoto (1), T. Matsuda (2)., 他12名
    • Journal Title

      Sci. Rep

      Volume: 3巻 Issue: 1 Pages: 2629-2629

    • DOI

      10.1038/srep02629

    • NAID

      120006995014

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 収差を利用した化学発光3次元イメージング法の開発2017

    • Author(s)
      新井由之
    • Organizer
      第8回定量生物学の会年会
    • Place of Presentation
      岡崎カンファレンスセンター
    • Year and Date
      2017-01-07
    • Related Report
      2016 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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